Identification of novel wraparound transcripts in JC polyomavirus DOI Creative Commons
Shun Iida,

Kenta Takahashi,

Sohtaro Mine

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract JC polyomavirus (JCPyV) is a ubiquitous pathogen causing progressive multifocal leukoencephalopathy (PML) in immunocompromised individuals. Polyomaviruses (PyVs) utilize complex transcriptional regulation mechanisms to express diverse mRNAs from their compact, double-stranded circular DNA genomes. A recent study employed next-generation sequencing (NGS) provide detailed transcriptome atlas of PyVs, including BK and simian virus 40. However, information regarding the JCPyV remains limited. Here, we conducted comprehensive analysis combining short-read long-read NGS methods unveil JCPyV. RNA isolated IMR-32 293 cells transfected with genome was analyzed by NGS, leading discovery 39 novel viral transcripts addition 12 previously annotated ones. Among these transcripts, identified characteristic wraparound conserved across which are constructed long primary generated through continuous, multiple rounds transcription genome. These included both late harboring leader-to-leader repeated sequences SuperT containing LxCxE motifs. Notably, transcript, were also detected brain tissues PML patients. Collectively, this significantly expands our understanding transcriptome, revealing expression lesions. findings valuable insights into molecular basis gene pathogenesis, potentially facilitating development effective countermeasures against PML. Author Summary first 1971 tissue patient (PML), life-threatening neurological disease. Despite over 50 years since pathogen’s identification, prognosis for extremely poor due lack therapeutics. Elucidating can expand providing targets therapeutic strategies. combination three technologies explore creating We ones cultured arise Wraparound characterized presence tandem repeats approximately 100 nucleotides. Our provides new expression, contributing

Language: Английский

Polyomavirus Infection in Humans: Epidemiology, Viral Aspects, and Disease DOI

Elias Myrvoll Lorentzen,

Hans H. Hirsch, Christine Hanssen Rinaldo

et al.

Springer eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 75

Published: Jan. 1, 2025

Language: Английский

Citations

0
Chemokine-mediated cell migration into the central nervous system in progressive multifocal leukoencephalopathy DOI Creative Commons

Marie Deffner,

Tilman Schneider-Hohendorf,

Andreas Schulte‐Mecklenbeck

et al.

Cell Reports Medicine, Journal Year: 2024, Volume and Issue: 5(7), P. 101622 - 101622

Published: June 24, 2024

Progressive multifocal leukoencephalopathy (PML) has been associated with different forms of immune compromise. This study analyzes the chemokine signals and attracted cells in cerebrospinal fluid (CSF) during PML to define cell subpopulations relevant for response. In addition chemokines that indicate a general state inflammation, like CCL5 CXCL10, CSF patients specifically contains CCL2 CCL4. Single-cell transcriptomics suggests an enrichment distinct CD4

Language: Английский

Citations

3
Causal relationship between genetically predicted antibody-Mediated Immune Responses and female infertility DOI

Wenyu Mo,

Jingjing Zhang, Xiaobin Peng

et al.

Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 166, P. 104319 - 104319

Published: Aug. 22, 2024

Language: Английский

Citations

1
Identification of novel wraparound transcripts in JC polyomavirus DOI Creative Commons
Shun Iida,

Kenta Takahashi,

Sohtaro Mine

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 30, 2024

Abstract JC polyomavirus (JCPyV) is a ubiquitous pathogen causing progressive multifocal leukoencephalopathy (PML) in immunocompromised individuals. Polyomaviruses (PyVs) utilize complex transcriptional regulation mechanisms to express diverse mRNAs from their compact, double-stranded circular DNA genomes. A recent study employed next-generation sequencing (NGS) provide detailed transcriptome atlas of PyVs, including BK and simian virus 40. However, information regarding the JCPyV remains limited. Here, we conducted comprehensive analysis combining short-read long-read NGS methods unveil JCPyV. RNA isolated IMR-32 293 cells transfected with genome was analyzed by NGS, leading discovery 39 novel viral transcripts addition 12 previously annotated ones. Among these transcripts, identified characteristic wraparound conserved across which are constructed long primary generated through continuous, multiple rounds transcription genome. These included both late harboring leader-to-leader repeated sequences SuperT containing LxCxE motifs. Notably, transcript, were also detected brain tissues PML patients. Collectively, this significantly expands our understanding transcriptome, revealing expression lesions. findings valuable insights into molecular basis gene pathogenesis, potentially facilitating development effective countermeasures against PML. Author Summary first 1971 tissue patient (PML), life-threatening neurological disease. Despite over 50 years since pathogen’s identification, prognosis for extremely poor due lack therapeutics. Elucidating can expand providing targets therapeutic strategies. combination three technologies explore creating We ones cultured arise Wraparound characterized presence tandem repeats approximately 100 nucleotides. Our provides new expression, contributing

Language: Английский

Citations

0