Deep sea treasures - insights from museum archives shed light on coral microbial diversity within deepest ocean ecosystems DOI Creative Commons
Francesco Ricci, William Leggat, Marisa M. Pasella

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 5, 2023

Abstract Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phyla. The technical limitations and high cost deep surveys have made exploring environments biology inhabit them challenging. In spite widespread recognition Cnidaria's environmental importance in these ecosystems, microbial assemblage its role coral functioning only been studied for a few water corals. Here we explored prokaryotic diversity corals by recovering nucleic acids from museum archive specimens. Firstly, amplified sequenced V1-V3 regions 16S rRNA gene specimens held archival storage utilised to shed light on associated with seven families collected depth Coral Sea (depth range 1,309 2,959 metres) Southern Ocean 1,401 2,071 habitats. Most surprisingly, Cyanobacteria sequences were consistently six out both sampling locations, suggesting bacteria potentially ubiquitous members microbiome cold organisms. Additionally, show might benefit symbiotic associations chemosynthetic including nitrite, carbon monoxide sulfur oxidizers. Consistent previous studies, bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes Acidobacteriota dominated community sea. We also genomes genus Mycoplasma , which identified as three families, finding evidence may aid immune system. Importantly our results retain components can provide new insights into microbiomes (and other organisms), well microbes writ large ecosystems.

Language: Английский

Microbiome Stability Is Linked to Acropora Coral Thermotolerance in Northwestern Philippines DOI Open Access
Jake Ivan Baquiran, John Bennedick Quijano, Madeleine J. H. van Oppen

et al.

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(2)

Published: Jan. 31, 2025

Corals associate with a diverse community of prokaryotic symbionts that provide nutrition, antioxidants and other protective compounds to their host. However, the influence microbes on coral thermotolerance remains understudied. Here, we examined microbial communities associated colonies Acropora cf. tenuis exhibit high or low upon exposure 33°C (heated) relative 29°C (control). Using 16S rRNA sequencing, show structure all A. was similar each at control temperature. Thermotolerant colonies, however, had relatively greater abundance Endozoicomonas, Arcobacter, Bifidobacterium Lactobacillus. At elevated temperature, only thermosensitive showed distinct shift in microbiome, an increase Flavobacteriales, Rhodobacteraceae Vibrio, accompanying marked bleaching response. Functional prediction indicated thermotolerant corals were enriched for genes related metabolism, while microbiomes cell motility antibiotic compound synthesis. These differences may contribute variable performance under thermal stress. Identification taxa correlated provides insights into beneficial bacterial groups could be used microbiome engineering support reef health changing climate.

Language: Английский

Citations

1
Localization and symbiotic status of probiotics in the coral holobiont DOI Creative Commons
Pedro Cardoso,

L. Hill,

Helena D. M. Villela

et al.

mSystems, Journal Year: 2024, Volume and Issue: 9(5)

Published: April 12, 2024

ABSTRACT Corals establish symbiotic relationships with microorganisms, especially endosymbiotic photosynthetic algae. Although other microbes have been commonly detected in coral tissues, their identity and beneficial functions for host are unclear. Here, we confirm the outcomes of inoculation bacteria selected as probiotics use fluorescence situ hybridization (FISH) to define localization Pocillopora damicornis . Our results show first evidence inherent presence Halomonas sp. Cobetia native even before inoculation. Furthermore, relative enrichment these tissue-associated through corals correlates health improvements, such increases potential, productivity. study suggests status by indicating within gastrodermis epidermis correlating increased abundance active holobiont. This knowledge is crucial facilitate screening application that may not be transient members microbiome. IMPORTANCE Despite promising associated some scarce regarding bacterial cells found tissue, correlation between two aspects still missing. gap limits our understanding actual diversity coral-associated whether symbionts beneficial. Some researchers, example, suggesting probiotic should only focus on very few known bacteria, Endozoicomonas sp., assuming currently tested tissue-associated. provide specific FISH probes expand probiotics. The microorganisms (BMCs) inside tissues gastric cavities also supports notion direct interactions underpin role. a new breakthrough; argue against possibility positive effects BMCs due factors related interaction, simply feeds inoculated or change water quality.

Language: Английский

Citations

4
Deep sea treasures - Insights from museum archives shed light on coral microbial diversity within deepest ocean ecosystems DOI Creative Commons
Francesco Ricci, William Leggat, Marisa M. Pasella

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(5), P. e27513 - e27513

Published: March 1, 2024

Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phylum. The technical limitations and high cost deep surveys have made exploring environments biology inhabit them challenging. In spite widespread recognition Cnidaria's environmental importance in these ecosystems, microbial assemblage its role coral functioning only been studied for a few water corals. Here, we explored diversity corals by recovering nucleic acids from museum archive specimens. Firstly, amplified sequenced V1–V3 regions 16S rRNA gene specimens, then utilized generated sequences to shed light on associated with seven families collected depth Coral Sea (depth range 1309 2959 m) Southern Ocean 1401 2071 habitats. Surprisingly, Cyanobacteria were consistently six out both sampling locations, suggesting bacteria potentially ubiquitous members microbiome cold Additionally, show might benefit symbiotic associations chemosynthetic including nitrite, carbon monoxide sulfur oxidizers. Consistent previous studies, bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes Acidobacteriota dominated community sea. We also genomes genus Mycoplasma, which identified as specimens three families, finding evidence may aid immune system. Importantly our results retain components can provide new insights into microbiomes (and other organisms), well microbes writ large ecosystems.

Language: Английский

Citations

0
Deep sea treasures - insights from museum archives shed light on coral microbial diversity within deepest ocean ecosystems DOI Creative Commons
Francesco Ricci, William Leggat, Marisa M. Pasella

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 5, 2023

Abstract Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phyla. The technical limitations and high cost deep surveys have made exploring environments biology inhabit them challenging. In spite widespread recognition Cnidaria's environmental importance in these ecosystems, microbial assemblage its role coral functioning only been studied for a few water corals. Here we explored prokaryotic diversity corals by recovering nucleic acids from museum archive specimens. Firstly, amplified sequenced V1-V3 regions 16S rRNA gene specimens held archival storage utilised to shed light on associated with seven families collected depth Coral Sea (depth range 1,309 2,959 metres) Southern Ocean 1,401 2,071 habitats. Most surprisingly, Cyanobacteria sequences were consistently six out both sampling locations, suggesting bacteria potentially ubiquitous members microbiome cold organisms. Additionally, show might benefit symbiotic associations chemosynthetic including nitrite, carbon monoxide sulfur oxidizers. Consistent previous studies, bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes Acidobacteriota dominated community sea. We also genomes genus Mycoplasma , which identified as three families, finding evidence may aid immune system. Importantly our results retain components can provide new insights into microbiomes (and other organisms), well microbes writ large ecosystems.

Language: Английский

Citations

0