Temperature modulates 17β-estradiol regulation of oogenesis protein expression in the liver of the eurythermal pupfish Cyprinodon nevadensis

General and Comparative Endocrinology, Journal Year: 2025, Volume and Issue: unknown, P. 114707 - 114707

Published: March 1, 2025

Female fish experiencing atypically high or prolonged elevations in temperature during oogenesis can suffer impaired oocyte development with fewer smaller eggs, eggs reduced yolk content thinner envelopes, and lower egg viability. These changes quality quantity are part caused by diminished liver synthesis of (vitellogenin, Vtg) envelope (choriogenin) proteins at anomalously temperatures. Those declines Vtg choriogenin production commonly paralleled blood concentrations 17β-estradiol (E2). However, it is unclear whether vitellogenin elevated temperatures result solely from circulating E2 if other aspects signaling also altered to diminish proteins. In this study, adult female Amargosa River pupfish (Cyprinodon nevadensis amargosae), a species asynchronous follicular development, were maintained 20 °C, 28 36 °C then administered vehicle solution. Ovarian gonadosomatic index (GSI) values plasma females compared those cooler Females had protein, abundances for mRNAs encoding genes (vtgAa, vtgAb, vtgc), (cgh, cghm, cgl), estrogen receptor α (esr1). Supplemental increased all temperatures, but only upregulated despite upregulation hepatic esr1 transcripts exhibited higher mRNA sex hormone-binding globulin (shbg) cytochrome P450 family 1 subfamily A member (cyp1a1), an estrogen-metabolizing monooxygenase enzyme that converts 2-hydroxyestradiol. Together, these findings indicate stimulation expression via effects on several including as well shbg cyp1a1 expression, which may free:bound the rate inactivation. results demonstrate replacement help compensate temperature-induced gene pupfish.

Language: Английский

Reproductive Traits of Five-Line Snapper, Lutjanus quinquelineatus (Bloch, 1790) (Actinopterygii: Perciformes: Lutjanidae), from Southern South China Sea, Malaysia

Thalassas An International Journal of Marine Sciences, Journal Year: 2024, Volume and Issue: 40(3), P. 1421 - 1432

Published: July 30, 2024

Language: Английский