Landscape influence on pollinator population genetic connectivity

Insect Conservation and Diversity, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Abstract Insect pollinators face numerous threats, including habitat loss and population fragmentation. The effects of human‐altered landscapes on connectivity need to be better understood inform effective mitigation measures. We examined the literature landscape heterogeneity genetic in two key pollinator groups: bees (Hymenoptera: Anthophila) hoverflies (Diptera: Syrphidae). identified 113 studies from 59 countries, covering 96 bee 21 hoverfly species. However, biased taxonomic geographical coverage limited broad conclusions regarding species susceptibility isolation. Notably, remain significantly understudied, hampering comprehensive assessments patterns. While some demonstrated differentiation across tens kilometres, others maintained entire continents. Various features, water bodies mountain ranges, often acted as barriers gene flow, while impacts deforestation, agriculture, urbanisation were mixed. Biological traits like body size, resource specialisation, sizes found influence contrasting results precluded conclusive findings. Future research should include evaluations time lags statistical power determine appropriateness selected tools for testing hypotheses recent pollinators. Owing increased interest corridors, encompassing a wider range species, habitats, improved study designs, is needed provide an evidence‐based framework conservation

Language: Английский

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Whole genome demographic models indicate divergent effective population size histories shape contemporary genetic diversity gradients in a montane bumble bee

Ecology and Evolution, Journal Year: 2023, Volume and Issue: 13(2)

Published: Jan. 31, 2023

Understanding historical range shifts and population size variation provides an important context for interpreting contemporary genetic diversity. Methods to predict changes in species distributions model effective (

Language: Английский

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The integration of whole‐genome resequencing and ecological niche modelling to conserve profiles of local adaptation

Diversity and Distributions, Journal Year: 2024, Volume and Issue: 30(6)

Published: April 11, 2024

Abstract Background Ecological and genomic attributes of populations can provide two orthologous perspectives on the biological profiles associated with local adaptation. The ability organisms to track suitable habitats (ecological adaptability) shift allele frequencies (adaptive potential) are prerequisite for population sustainability. Aims Many contemporary threatened by habitat loss vulnerability) a lack adaptive potential (evolutionary vulnerability). Technical advances new opportunities address these challenges in conservation: Future shifts be predicted ecological niche modelling genetic diversity discerned using genome sequence data. Together, approaches illuminate adaptation profile help identify environmental conditions that should maximize evolutionary fitness. Materials Methods Here, we reviewed primary literature key studies utilize both whole‐genome resequencing (WGR) (ENM) an effort envisage future research directions may benefit conservation efforts. Results We identified ways integrate different approaches, such as ENM‐informed genomics genomics‐informed ENMs, used delineate conserve profiles. Discussion Integrative characteristics, vulnerable subject changes, patterns from geographic analyses. discuss directions, limitations their solutions suggestions collaborative workflows. Conclusion integration WGR ENM is promising continuous advancement. An integrative approach evaluate eco‐evolutionary attributes, at organismal molecular levels,

Language: Английский

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De novo genome assembly and annotations of Bombus lapidarius and Bombus niveatus provide insights into the environmental adaptability

Apidologie, Journal Year: 2025, Volume and Issue: 56(1)

Published: Jan. 21, 2025

Abstract Bumblebees are ubiquitous, cold-adapted, primitively eusocial bees and important pollinators for crops vegetation. However, many species declining worldwide due to multiple factors, including human-induced habitat loss, agricultural chemicals, global warming, climate change. In particular, future scenarios predict a shift in the spatial distribution of bumblebees under with some others potentially expanding. Here, we report de novo genome assembly annotation Bombus lapidarius niveatus decipher species-specific potential genomic capacity against such environmental stressors. With harboring more than 23,000 protein-coding genes, assembled genomes B. 244.44 Mb (scaffold N50 9.45 Mb) 259.84 10.94 Mb), respectively, which exhibit similar trends terms size composition other bumblebees. Gene family analysis reveals differences expanded gene families. exhibits genes related pre/postsynaptic organization, while shows distinct expansion families regulating cellular growth, aging, responses abiotic biotic stressors, as those containing SCAN domains, WD-repeats, Ras-related proteins. Our genome-wide screens revealed positive selection on stress-responsive dip2 , yme1l spg7 whereas signatures were found myd88 mybbp1A rhau involved stress resistance . These high-quality assemblies comparative unveil drivers that underlie evolution bumblebees, offering valuable insights into adaptation conservation efforts.

Language: Английский

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A rapid return to normal: temporal gene expression patterns following cold exposure in the bumble bee Bombus impatiens

Journal of Experimental Biology, Journal Year: 2024, Volume and Issue: 227(9)

Published: April 17, 2024

ABSTRACT Bumble bees are common in cooler climates and many species likely experience periodic exposure to very cold temperatures, but little is known about the temporal dynamics of response mechanisms following chill exposure, especially how persistent effects may facilitate tolerance future events. To investigate molecular processes involved by bumble acute we compared mRNA transcript abundance Bombus impatiens workers exposed 0°C for 75 min (inducing coma) control maintained at a constant ambient temperature (28°C). We sequenced 3′ end transcripts (TagSeq) quantify gene expression thoracic tissue several time points (0, 10, 30, 120 720 min) exposure. Significant differences from were only detectable within 30 after treatment, with most occurring 10 recovery point. Genes associated gluconeogenesis glycolysis notably upregulated, while genes related lipid purine metabolism downregulated. The observed patterns indicate rapid coma, suggesting an differential transcriptional during coma return baseline levels hour, no long-term markers this Our work highlights functions pathways important recovery, provides estimated frame bees, suggests that hardening be less these heterothermic insects.

Language: Английский

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Population delimitation in bumble bees - strategies and research gaps

Frontiers in Bee Science, Journal Year: 2025, Volume and Issue: 3

Published: March 7, 2025

Declining social insects such as bumble bees are particularly vulnerable to loss of genetic diversity. Population delimitation is a precondition for measuring diversity but usually requires extensive data and comprehensive knowledge about gene flow barriers. As first step towards strategy that does not rely on data, we compiled existing Bombus population structures (potential) We reviewed studies examining structuring in species assessed the impact different ecological environmental factors their flow. Generally, found declining clearly isolated populations exhibit which some underlying can be correlated with isolation-by-landscape approaches. For widespread species, isolation-by-environment approaches help elucidate subtle impeding between populations, even though capable maintaining across large stepping stone populations. However, better inform isolation-by landscape/environment models could contribute landscape-based strategy, more research into habitat requirements dispersal ranges has conducted.

Language: Английский

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The complete genome of Tetragonisca angustula (Apidae: Meliponini), the most commonly reared stingless bee in Brazil

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Abstract Background The field of bee genomics has been considerably advanced in recent years, however, the most diverse group honey producers on planet, stingless bees (SLBs), are still largely neglected. In fact, only ten ~600 described SLB species have sequenced, and one using a long-read (LR) sequencing technology. Here, we sequenced complete genome common, widespread broadly reared Brazil— Tetragonisca angustula (popularly known as jataí). Results A total 48.01 Gb DNA data were generated, including 2.31 Pacific Bioscience LRs 45.70 Illumina short reads (SRs). Our preferred assembly comprised 705 contigs encompassing 283.99 Mb, 65.94 Mb which (23.22%) corresponded to 462,261 repetitive elements. N50, L50 BUSCOs reached 1.01 94 97%, respectively. We predicted that T. comprises 16,958 protein-coding genes 1,866 non-coding RNAs. mitogenome consisted 17,410 bp, all 37 found be positive strand, an unusual feature among bees. phylogenomic analysis 26 hymenopteran revealed six 64 orthogroups experiencing rapid evolution included odorant receptor genes, but undergoing considerable contractions. Conclusions provided first for ecologically economically important , second with LR technology thus far. demonstrated even relatively small amounts combination sufficient SR can yield high-quality assemblies

Language: Английский

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The nuclear and mitochondrial genome assemblies of Tetragonisca angustula (Apidae: Meliponini), a tiny yet remarkable pollinator in the Neotropics

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: June 11, 2024

Abstract Background The field of bee genomics has considerably advanced in recent years, however, the most diverse group honey producers on planet, stingless bees, are still largely neglected. In fact, only eleven ~ 600 described species have been sequenced, and three using a long-read (LR) sequencing technology. Here, we sequenced nuclear mitochondrial genomes common, widespread broadly reared Brazil other neotropical countries— Tetragonisca angustula (popularly known as jataí). Results A total 48.01 Gb DNA data were generated, including 2.31 Pacific Bioscience HiFi reads 45.70 Illumina short (SRs). Our preferred assembly comprised 683 contigs encompassing 284.49 Mb, 62.84 Mb which (22.09%) corresponded to 445,793 repetitive elements. N50, L50 complete BUSCOs reached 1.02 91 97.1%, respectively. We predicted that genome T. comprises 17,459 protein-coding genes 4,108 non-coding RNAs. mitogenome consisted 17,410 bp, all 37 found be positive strand, an unusual feature among bees. phylogenomic analysis 26 hymenopteran revealed six odorant receptor orthogroups experiencing rapid evolution, four them undergoing significant contractions. Conclusions provided first assemblies for ecologically economically important , fourth with LR technology thus far. demonstrated even relatively small amounts combination sufficient SR can yield high-quality

Language: Английский

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Whole genome analyses reveal weak signatures of population structure and environmentally associated local adaptation in an important North American pollinator, the bumble bee Bombus vosnesenskii

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(20), P. 5479 - 5497

Published: Sept. 13, 2023

Studies of species that experience environmental heterogeneity across their distributions have become an important tool for understanding mechanisms adaptation and predicting responses to climate change. We examine population structure, demographic history environmentally associated genomic variation in Bombus vosnesenskii, a common bumble bee the western USA, using whole genome resequencing populations distributed broad range latitudes elevations. find B. vosnesenskii exhibits minimal structure weak isolation by distance, confirming results from previous studies other molecular marker types. Similarly, analyses with Sequentially Markovian Coalescent models suggest may persisted since last interglacial period, genomes different parts showing similar historical effective size trajectories relatively small fluctuations through time. Redundancy analysis revealed amount explained bioclimatic variables. Environmental association latent factor mixed modelling (LFMM2) identified few outlier loci were sparsely throughout although putative signatures selective sweeps identified, none encompassed particularly large numbers loci. Some genes known regulatory relationships, suggesting possibility selection, compared examined approaches, evidence extensive local was weak. Overall, indicate is example generalist high degree flexibility its requirements ultimately benefit under periods

Language: Английский

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Pan‐genome analysis highlights the role of structural variation in the evolution and environmental adaptation of Asian honeybees

Molecular Ecology Resources, Journal Year: 2023, Volume and Issue: 24(2)

Published: Nov. 23, 2023

The Asian honeybee, Apis cerana, is an ecologically and economically important pollinator. Mapping its genetic variation key to understanding population-level health, histories potential capacities respond environmental changes. However, most efforts date were focused on single nucleotide polymorphisms (SNPs) based a reference genome, thereby ignoring larger scale genomic variation. We employed long-read sequencing technologies generate chromosome-scale genome for the ancestral group of A. cerana. Integrating this with 525 resequencing data sets, we constructed first pan-genome encompassing almost entire gene content. found that 31.32% genes in variably present across populations, providing broad pool adaptation. identified characterized structural variations (SVs) they not closely linked SNP distributions; however, formation SVs was associated transposable elements. Furthermore, phylogenetic analysis using revealed novel cerana ecological recoverable from data. Performing association total 44 likely be Verification one these, 330 bp deletion Atpalpha gene, indicated SV may promote cold adaptation by altering expression. Taken together, our study demonstrates feasibility utility applying approaches map explore feature honeybee particular examine role evolution

Language: Английский

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