Biodegradation of S-Triazine Herbicides Under Saline Conditions by Paenarthrobacter ureafaciens PC, a New Halotolerant Bacterial Isolate: Insights into Both the Degradative Pathway and Mechanisms of Tolerance to High Salt Concentrations

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 649 - 649

Published: March 12, 2025

In this study, a halotolerant bacterial strain was isolated and identified. This bacterium confirmed to efficiently degrade s-triazine herbicides under saline conditions. The optimal conditions for the metabolism growth of were determined through single-factor tests. Furthermore, biodegradation pathways prometryne (the target compound) by proposed based on detection possible degradation intermediates genome sequencing analysis. Additionally, halotolerance mechanisms also revealed screening halotolerance-related genes in its genome. results demonstrated that (designated PC), which completely degraded 20.00 mg/L within 12 h (30.0 g/L NaCl), identified as Paenarthrobacter ureafaciens. PC follows: yeast extract additional carbon source with concentration ≥0.1 g/L, NaCl ≤30.0 initial pH 7.0, temperature 35.0 °C, shaking speed ≥160 rpm. efficient removal other herbicides, including atrazine, ametryne, simetryne, cyanazine. might series steps, demethylthiolation, deisopropylamination, deamination, dealkalation, decarboxylation, etc., relying relevant functional involved compounds. tolerate high salinity excessive uptake K+ into cells, intracellular accumulation compatible solutes, production halophilic enzymes. study is expected provide potentially effective purifying pollutants environments.

Language: Английский

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Novel and diverse features identified in the genomes of bacteria isolated from a hydrothermal vent plume

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

ABSTRACT Hydrothermal vent plumes (HVPs), formed by high-temperature emissions, are rich in compounds that support chemosynthesis and serve as reservoirs of microbial diversity genetic innovation. Through turbulence, mixing, interaction with subsea currents, communities thought to disperse across ocean basins. In this study, we focused on the plume Moytirra hydrothermal field, a relatively unexplored site, investigate its inhabitants. We cultured bacteria from HVP using 11 different media types performed complete genome sequencing 12 isolates. Our analyses revealed four putatively novel species Thalassobaculum , Sulfitobacter Idiomarina Christiangramia genera. Comparative genomics identified unique genomic islands containing biosynthetic gene clusters, including Non-Ribosomal Peptide Synthetase/Polyketide Synthase cluster, toxin-antitoxin systems, evidence horizontal transfer facilitated prophages. These findings underscore potential HVPs source biotechnologically relevant genes, contributing our understanding biodiversity complexity these extreme environments. IMPORTANCE vents dynamic environments offer nutrients for chemosynthetic organisms drive biology deep-sea. The dynamics ecosystems innovation resident populations. (HVPs) mix surrounding water, carrying local microbiota them dispersing hundreds kilometers. This study isolated capture snapshot community, revealing within three taxonomic classes. addition genomes public databases provides valuable insights into function, architecture, clusters found

Language: Английский

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Insights into atrazine degradation by a halotolerant bacterium Arthrobacter sp. PSC in high-salt aquatic environments: degradation pathway and halotolerance mechanism elucidation

Journal of Water Process Engineering, Journal Year: 2025, Volume and Issue: 73, P. 107702 - 107702

Published: April 14, 2025

Language: Английский

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Large diversity in the O-chain biosynthetic cluster within populations of Pelagibacterales

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

ABSTRACT Genomic diversity in prokaryotic species is largely due to the existence of extensive pangenomes, allowing different gene complements be drawn depending on strain. Here, we have studied O-chain polysaccharide biosynthesis cluster (OBC) marine bacteria Pelagibacterales order as a proxy measure such genetic single population. The study single-amplified genomes (SAGs) from whole found pattern similar that other well-studied microbes, Enterobacteriales or Alteromonas , where distinct OBCs represent strains containing pools. We most OBC sharing happened among individuals same clonal frame (>99% average nucleotide identity). Moreover, given parsimonious way this changes, can extrapolated size population’s pangenome. This assumes correspond lineages unique flexible pools, seen aforementioned microbes. Through long-read metagenomics, could detect 380 at Mediterranean sampling site. Within population (single and sample) endemic Ia.3/VII (gMED) genomospecies, identified 158 OBCs, which 130 were unique. These findings suggest pool within might substantial (several thousands). While figure large, it aligns with complexity dissolved organic matter these organisms potentially degrade. IMPORTANCE Different bacterial contain very has been long known by epidemiologists. However, unknown what present set environmental conditions, i.e., time place free-living bacteria. leveraged information SAGs analyze coding for polysaccharide, typical component classically used tool differentiate clinical microbiology. It evolves rate rest genome does not seem affected an arms race phages. One Pelagibacteriales revealed astounding one sample metagenomics. Our results point large (local pangenome) population, critical interpreting biological meaning pangenome, i.e ., provides intrapopulation rather than characterizing distribution and/or space.

Language: Английский

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Large diversity in the O-chain biosynthetic cluster within populations of Pelagibacterales

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

ABSTRACT We have used single-amplified genomes (SAGs) and long-read metagenomics to examine the diversity of O-chain polysaccharide biosynthesis cluster (OBC) in marine bacteria Pelagibacterales order. OBCs are notorious for their been type strains pathogens saprophytes, but patterns variation free-living little known. found that, these heterotrophic bacteria, is comparable that such as Enterobacteriales i.e. nearly each strain carries a different OBC. However, although OBC inheritance was largely vertical, existence some shared clusters allowed comparative analysis. The diverge along with genome, which taken indicative old horizontal gene transfer (HGT) events. Only 14 cases recent HGT were detected they happened independently taxonomy or location. Thus, major phage receptor Gram-negative exchange complete seems play minor role phage-bacterium arms-race. By metagenomics, we could detect 380 single sampling site Mediterranean. A population (single species sample) endemic Ia.3/VII genomospecies had set 158 130 different. This large clonal lineages might reflect amount metabolic pathways required deal enormous chemical dissolved organic matter ocean.

Language: Английский

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Plateau Pika Disturbance Changes Soil Bacterial Functions and Exoenzyme Abundance to Modulate the C Cycle Pathway in Alpine Grasslands

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12775 - 12775

Published: Nov. 28, 2024

Plateau pika (Ochotona curzoniae) is crucial to soil organic carbon (SOC) storage in the Qinghai–Tibetan plateau (QTP), but its role bacterial SOC metabolisms across different degraded alpine grasslands remains unclear. In this study, we investigated physicochemical properties and composition function of communities control pika-disturbed experiencing degradation levels (undegraded, UDM; lightly, LDM; moderately, MDM severely, SDM). The results demonstrate that (i) primary phyla include Proteobacteria, Acidobacteriota, Actinobacteriota, Bacteroidota Verrucomicrobiota. Soil significantly impact determine influence disturbance. Pika disturbance increases OTUs by 7.5% LDP (p > 0.05) 50.5% MDP < 0.05), while decreases 21.4% SDP 0.05). (ii) downregulates exoenzyme abundance associated with simple complex matter decomposition 9.5% 13.9% LDP, 29.4% 26.3% upregulates these exoenzymes 23.6% 37.9% These changes correspond increase TC declines SDP. (iii) can enhance accumulation through upregulating C cycle pathway ethanol production MDP. However, it pyruvate CO2 conversion SDP, leading negative on storage.

Language: Английский

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Global freshwater distribution of Telonemia protists

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Language: Английский

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