Unveiling Detoxifying Symbiosis and Dietary Influence on the Southern Green Shield Bug Microbiota DOI Creative Commons
Magda A. Rogowska‐van der Molen,

Hristina V Savova,

Edelgard Janssen

et al.

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(12)

Published: Nov. 7, 2024

Abstract The Southern green shield bug, Nezara viridula, is an invasive piercing and sucking pest insect that feeds on crops poses a threat to global food production. Insects live in close relationships with microorganisms providing their host unique capabilities, such as resistance toxic plant metabolites. In this study, we investigated the detoxification of metabolite 3-nitropropionic acid (NPA) by core transient members N. viridula microbial community. Microbial community showed different tolerance toxin determined six out eight strains detoxified NPA. Additionally, NPA might interfere biosynthesis transport l-leucine. Moreover, our study explored influence diet gut composition demonstrating switching single-plant shifts abundance microbes. line this, testing pairwise interactions revealed microbiota support each other repress growth microorganisms. With work, provide novel insights into factors shaping communities demonstrate harbours many toxin-degrading bacteria could its defences.

Language: Английский

The role of host plants in driving pathogen susceptibility in insects through chemicals, immune responses and microbiota DOI Open Access
Ehsan Sanaei,

Jacobus C. de Roode

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

In this comprehensive exploration, we delve into the pivotal role of host plants in shaping intricate interactions between herbivorous insects and their pathogens. Recent decades have seen a surge studies that demonstrate are crucial drivers pathogens, providing novel insights direct indirect shape tri-trophic interactions. These built on wide range from viruses to bacteria, protozoans fungi. We summarise these studies, discuss mechanisms plant-mediated insect resistance infection, ranging toxicity plant chemicals pathogens enhancement anti-pathogen immune responses, modulation insect's microbiome. Although provide evidence for roles all mechanisms, also point out majority existing phenomenological, describing patterns without addressing underlying mechanisms. To further our understanding interactions, therefore urge researchers design enable them specifically distinguish by which affect susceptibility

Language: Английский

Citations

0
Inside the Belly of the Beast: Exploring the Gut Bacterial Diversity of Gonipterus sp. n. 2 DOI Creative Commons
Rosa S Knoppersen, T. K. Bose, Teresa A. Coutinho

et al.

Microbial Ecology, Journal Year: 2025, Volume and Issue: 88(1)

Published: April 12, 2025

The Eucalyptus snout beetle (Gonipterus sp. n. 2) is a destructive invasive pest of plantations, responsible for significant defoliation and wood yield losses globally. Native to Australia, this has adapted thrive on diverse hosts, overcoming their chemical defences. However, the mechanisms by which Gonipterus tolerates or utilises these plant defence metabolites remain poorly understood. In South Africa, 2 poses threat plantations causing extensive leading substantial reductions in growth production. This study investigates relationship between diet, host species, gut microbiome 2. Using controlled feeding experiments, beetles were reared artificial, semi-artificial, natural diets, as well two genotypes with distinct secondary metabolite profiles. High-throughput 16S rDNA sequencing gas chromatography-mass spectrometry (GC-MS) revealed shifts bacterial diversity composition across diets. Natural diets supported most microbial communities, while artificial fostered homogenised dominated opportunistic taxa like Serratia. Host-specific effects observed frass microbiota, biotransformation monoterpenes into less toxic derivatives. results highlight plasticity enables metabolic adaptability resilience environments. flexibility underpins invasiveness Gonipterus, emphasising role symbionts Understanding interactions offers novel insights microbiome-targeted management strategies, providing sustainable approach mitigate impact global forestry.

Language: Английский

Citations

0
Parasitoid Calyx Fluid and Venom Affect Bacterial Communities in Their Lepidopteran Host Labial Salivary Glands DOI Creative Commons
Maximilien A. C. Cuny, Gabriele Gloder, Mitchel E. Bourne

et al.

Microbial Ecology, Journal Year: 2025, Volume and Issue: 88(1)

Published: April 23, 2025

Language: Английский

Citations

0
Seasonal Changes in the Gut Microbiota of Halyomorpha halys DOI Creative Commons
Maja Fluch, Erika Corretto, Heike Feldhaar

et al.

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Dec. 1, 2024

The gut microbiome plays an important role in insect evolution and ecology. Bacteria support the host's nutrition defense therefore play fitness of host. Halyomorpha halys is one most invasive pest species world. Native to North-Eastern Asia, this Pentatomid bug has recently invaded North America Europe, causing significant damage agricultural production. Although increasing number studies investigated biology species, little known about composition its microbiota. Like many other H. harbors a primary symbiont called "Candidatus Pantoea carbekii," which produces vitamins essential amino acids for However, information presence bacteria currently lacking. Therefore, we microbiota individuals, were collected field across year using high-throughput 16S rRNA gene metabarcoding approach. Our results revealed 3309 different ASVs associated with halys, being abundant symbiont, present almost all individuals. Additionally, individuals harbor Commensalibacter, genus acetic acid bacterial symbionts. Besides these two predominant taxa, show high diversity microorganisms seasonal fluctuations, highlighting dynamic that might influence species.

Language: Английский

Citations

1
Seasonal shifts in the gut microbiome of Halyomorpha halys DOI
Maja Fluch, Erika Corretto, Heike Feldhaar

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Abstract The gut microbiome plays an important role in insect evolution and ecology. Bacteria support the host nutrition defense therefore play for fitness of host. Halyomorpha halys is one most invasive pest species world. Native to North-Eastern Asia, this Pentatomid bug has recently invaded North America Europe, causing significant damage agricultural production. Although increasing number studies investigated biology species, little known about its associated microorganisms. Like many other H. harbors a primary symbiont called ‘Candidatus Pantoea carbekii’, that produces vitamins essential amino acids However, information presence bacteria currently lacking. Therefore, we individuals, which were collected field across year using high-throughput 16S rRNA gene metabarcoding approach. Our results revealed 645 different ASVs with halys, Pantoea being abundant symbiont, present almost all individuals. Interestingly, several individuals summer harbor Commensalibacter. Besides these two predominant taxa, show high diversity microorganisms seasonal fluctuations, highlighting dynamic might influence species.

Language: Английский

Citations

0
From Eggs to Guts: Symbiotic Association of Sodalis nezarae sp. nov. with the Southern Green Shield Bug Nezara viridula DOI Creative Commons
Magda A. Rogowska‐van der Molen, Alejandro Manzano-Marı́n, Jelle Postma

et al.

Published: Oct. 21, 2024

Abstract Phytophagous insects engage in symbiotic relationships with bacteria that contribute to digestion, nutrient supplementation, and development of the host. The analysis shield bug microbiomes has been mainly focused on gut intestinal tract predominantly colonized by Pantoea symbionts, other microbial community members or organs have hardly investigated. In this study, we reveal Southern green Nezara viridula harbours a Sodalis symbiont several organs, notable prevalence salivary glands, anterior regions midgut. Removing external egg microbiota via sterilization profoundly impacted insect viability but did not disrupt vertical transmission symbionts. Based dominance testes, deduce N. males could be involved transmission. Genomic analyses comparing species revealed sp. Nvir shares characteristics both free- living obligate insect-associated spp. also displays genome instability typical endosymbiont lineages, which suggests ongoing speciation an endosymbiont. Together, our study reveals bugs harbour unrecognized symbionts might paternally transmitted.

Language: Английский

Citations

0
Unveiling Detoxifying Symbiosis and Dietary Influence on the Southern Green Shield Bug Microbiota DOI Creative Commons
Magda A. Rogowska‐van der Molen,

Hristina V Savova,

Edelgard Janssen

et al.

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(12)

Published: Nov. 7, 2024

Abstract The Southern green shield bug, Nezara viridula, is an invasive piercing and sucking pest insect that feeds on crops poses a threat to global food production. Insects live in close relationships with microorganisms providing their host unique capabilities, such as resistance toxic plant metabolites. In this study, we investigated the detoxification of metabolite 3-nitropropionic acid (NPA) by core transient members N. viridula microbial community. Microbial community showed different tolerance toxin determined six out eight strains detoxified NPA. Additionally, NPA might interfere biosynthesis transport l-leucine. Moreover, our study explored influence diet gut composition demonstrating switching single-plant shifts abundance microbes. line this, testing pairwise interactions revealed microbiota support each other repress growth microorganisms. With work, provide novel insights into factors shaping communities demonstrate harbours many toxin-degrading bacteria could its defences.

Language: Английский

Citations

0