Effect of chitosan on buck semen quality and semen plasma metabolites during low-temperature storage DOI Creative Commons

Meijun Song,

Baoqing Jia,

Dinghui Dai

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 13, 2025

Background Optimizing buck semen preservation techniques can significantly advance the goat industry. This study aimed to investigate effects of chitosan on sperm quality and seminal plasma metabolite profiles in bucks during low-temperature storage at 4°C. Results The results showed that when 0.2 mg/mL was added dilution, viability antioxidant capacity were highest higher than control group ( p < 0.05). Sperm decreased progressively with increasing time However, day 5, all groups where dilutions A total 23 classes metabolites detected non-targeted metabolism plasma. caused by mainly included fatty acyls, phospholipids, amino acids organic acids. Most differential acyls glycerophospholipids chitosan-treated enriched anabolic pathway unsaturated Additionally, several oligopeptides correlations quality. Conclusion These suggest adding diluent successfully prolongs altering anabolism lipids provides theoretical support practical reference for applying semen.

Language: Английский

Effect of chitosan on buck semen quality and semen plasma metabolites during low-temperature storage DOI Creative Commons

Meijun Song,

Baoqing Jia,

Dinghui Dai

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: March 13, 2025

Background Optimizing buck semen preservation techniques can significantly advance the goat industry. This study aimed to investigate effects of chitosan on sperm quality and seminal plasma metabolite profiles in bucks during low-temperature storage at 4°C. Results The results showed that when 0.2 mg/mL was added dilution, viability antioxidant capacity were highest higher than control group ( p < 0.05). Sperm decreased progressively with increasing time However, day 5, all groups where dilutions A total 23 classes metabolites detected non-targeted metabolism plasma. caused by mainly included fatty acyls, phospholipids, amino acids organic acids. Most differential acyls glycerophospholipids chitosan-treated enriched anabolic pathway unsaturated Additionally, several oligopeptides correlations quality. Conclusion These suggest adding diluent successfully prolongs altering anabolism lipids provides theoretical support practical reference for applying semen.

Language: Английский

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