Dietary Supplementation with Fermented Milk Improves Growth Performance and Intestinal Functions in Intrauterine Growth-Restricted Piglets

Animals, Journal Year: 2025, Volume and Issue: 15(10), P. 1367 - 1367

Published: May 9, 2025

Intrauterine growth restriction (IUGR) commonly occurs in pigs and poses a significant challenge to the swine industry. This study investigated effect of fermented milk on performance intestinal health IUGR-affected piglets. A total 24 28-day-old weaned piglets with IUGR were randomly assigned corn-soybean basal diet (control) or mixed (3:1 w/v, treatment). The results showed that increased average daily gain decreased feed-to-gain ratio (p < 0.05). Fermented villus height duodenum jejunal crypt depth Pigs treatment higher activities lipase, α-amylase, sucrase duodenum, along an elevation activity ileal glutathione concentration was by Moreover, upregulated protein expression occludin claudin-3 while decreasing gene interleukin 1 beta, 6, tumor necrosis factor αlpha jejunum Collectively, these indicate dietary supplementation significantly improved through enhancement functions piglets, highlighting potential as nutritional strategy improve postnatal

Language: Английский

---

Dietary glycine supplementation enhances creatine availability in tissues of pigs with intrauterine growth restriction

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

This study tested the hypothesis that dietary supplementation with glycine (Gly) enhances synthesis and availability of creatine (Cr) in tissues pigs intrauterine growth restriction (IUGR). At weaning (21 d age), IUGR litter mates normal birth weights (NBW) were assigned randomly to 1 2 groups, namely, 1% Gly or 1.19% l-alanine (isonitrogenous control) a corn- soybean meal-based diet. Blood, kidneys, liver, pancreas, jejunum, longissimus lumborum muscle (LLM), gastrocnemius (GM) obtained from within wk after feeding trial ended at 188 age determine concentrations guanidinoacetate (GAA), Cr, creatinine, phosphocreatine (CrP). The organs also analyzed for activities mRNA levels Cr-synthetic enzymes: l-arginine:glycine amidinotransferase (AGAT; forming GAA l-arginine) n-methyltransferase (GAMT; converting l-methionine into Cr). AGAT activity was present whereas GAMT found all analyzed. most active per gram tissue kidneys respectively. Based on mass, had greatest (P < 0.001) whole organ, followed by while skeletal liver. Thus, played dominant role GAA, liver major sites Cr. Dietary enhanced pancreas but reduced small intestine, therefore directing Cr production. selectively concentration LLM among studied. Except GM greater than NBW pigs, neither nor affected selected genes examined. Collectively, these novel results indicate intake upregulates pathway swine.

Language: Английский

---

Dietary glycine supplementation activates mTOR signaling pathway in tissues of pigs with intrauterine growth restriction

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

Abstract The mechanistic target of rapamycin (mTOR) cell signaling pathway serves as the central mechanism for regulation tissue protein synthesis and growth. We recently reported that supplementing 1% glycine to corn- soybean meal-based diets enhanced growth performance between weaning market weights in pigs with intrauterine restriction (IUGR). Results recent studies have revealed an important role activating mTOR C2C12 muscle cells. Therefore, present study tested hypothesis dietary supplementation skeletal other tissues IUGR pigs. At (21 d age), litter mates normal birth (NBW) were assigned randomly one two groups: either or 1.19% l-alanine (isonitrogenous control) a diet. Tissues obtained from within 1 wk after feeding trial ended at 188 age determine abundances total phosphorylated forms its major downstream proteins: eukaryotic initiation factor 4E-binding protein-1 (4EBP1) ribosomal S6 kinase-1 (p70S6K). showed decreased (P &lt; 0.05) both mTOR, 4EBP1, p70S6K gastrocnemius jejunum. In longissimus lumborum pigs, did not differ &gt; but those 4EBP1 downregulated 0.05), when compared NBW These adverse effects muscle, jejunum prevented by supplementation. Interestingly, liver affected Collectively, our findings indicate impaired adequate intake was crucial maintaining active mTOR-dependent development muscle.

Language: Английский

---

Transmembrane Amino Acid Transporters in Shaping the Metabolic Profile of Breast Cancer Cell Lines: The Focus on Molecular Biological Subtype

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 47(1), P. 4 - 4

Published: Dec. 25, 2024

Amino acid metabolism in breast cancer cells is unique for each molecular biological subtype of cancer. In this review, the features cell are considered terms changes amino composition due to activity transmembrane transporters. addition main signaling pathway PI3K/Akt/mTOR, oncogene c-Myc, HIF, p53, GATA2, NF-kB and MAT2A have a direct effect on cells, their growth proliferation, as well maintenance homeostatic equilibrium. A distinctive feature luminal subtypes from TNBC ability perform gluconeogenesis. Breast cancers with positive expression HER2 receptor, contrast subtype, active synthesis consumption fatty acids. It interesting note that transporters exhibit depending pH level inside cell. most aggressive forms or gradual progression disease, will also change, which directly affect Using lines presented we can trace characteristic inherent develop optimal therapeutic targets.

Language: Английский

---

Metabolome and transcriptome profiling reveal tRNA-derived small RNAs regulated glutathione metabolism in intrauterine growth-restricted pigs liver

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139167 - 139167

Published: Dec. 1, 2024

Language: Английский

---

Glycine Intervention Alleviates Spermatogenic Injury Induced by Imidacloprid

Published: Jan. 1, 2024

Language: Английский

---

Low feed intake at weaning reduces intestinal glutathione levels and promotes cysteine oxidation to taurine in pigs

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

Weaning stress in pigs is associated with low feed intake and poor nutrient utilization. Cysteine a sulfur amino acid key roles pig production, but how cysteine metabolism requirements are affected by weaning should be better defined. The objective of this study was to determine the collective impact restriction on tissue metabolism. Pigs were weaned at 21-d age without access (W; 6.90 ± 0.81 kg; n = 9; reflecting acute nutritional stress) or not remained sow (nonweaned [NW]; 6.81 0.65 8). At euthanasia (23-d age), blood, bile, liver, jejunum, ileum tissues collected. Plasma, thiol concentrations analyzed HPLC. activity glutamate ligase (GCL) glutathione synthetase (GSS), enzymes needed for (GSH) dioxygenase 1 (CDO1) determined assays followed HPLC analysis reaction products. Plasma (271 vs. 192 19 µmol/L; P < 0.001) liver (417 298 33 nmol/g; 0.05) Cys increased W compared NW pigs. Despite greater plasma Cys, jejunum content (P > 0.10), whereas γ-glutamylcysteine (γ-GlyCys), immediate precursor GSH, declined both (14.3 9.7 1.4 0.01) (11.2 6.4 0.8 Glutathione lower (1,379 1,720 70 (1,497 1,740 74 In GCL tended (0.56 0.39 0.07 nmol γ-GluCys • mg-1 min-1; GSS (1.11 1.38 0.10 GSH 0.10) ileum, activities 0.10). Although CDO1 different between groups taurine (5,115 2,336 912 0.001). Bile (1,203 279 103 cysteinylglycine (203 117 direct product degradation, Collectively, these results suggest that systemic effectively utilized gut production newly pigs; instead, it oxidized eliminated bile.

Language: Английский

---