Proceedings of the National Academy of Sciences,
Journal Year:
2024,
Volume and Issue:
121(49)
Published: Nov. 27, 2024
Estrogen
deficiency,
which
is
linked
to
various
pathological
conditions
such
as
primary
ovarian
insufficiency
and
postmenopausal
osteoporosis,
disrupts
the
delicate
balance
between
bone
formation
resorption.
This
imbalance
leads
loss
an
increased
risk
of
fractures,
primarily
due
a
significant
reduction
in
trabecular
mass.
Trabecular
osteoblasts,
cells
responsible
for
within
compartment,
originate
from
skeletal
progenitors
located
marrow.
The
microenvironment
marrow
contains
hypoxic
(low
oxygen)
regions,
hypoxia-inducible
factor-2α
(HIF2)
plays
crucial
role
cellular
responses
these
low-oxygen
conditions.
study
demonstrates
that
HIF2
their
derivatives
during
development
enhances
mass
by
promoting
formation.
More
importantly,
PT2399,
small
molecule
specifically
inhibits
HIF2,
effectively
prevents
ovariectomized
adult
mice,
model
estrogen-deficient
loss.
Both
genetic
pharmacological
approaches
result
increase
osteoblast
number,
expansion
pool
progenitor
cells.
either
or
inhibition
uncovers
pivotal
mechanism
increasing
numbers
formation,
resulting
greater
Current Issues in Molecular Biology,
Journal Year:
2025,
Volume and Issue:
47(3), P. 175 - 175
Published: March 7, 2025
Marine-derived
biomaterials
are
emerging
as
promising
candidates
for
tissue
regeneration
due
to
their
sustainability,
biocompatibility,
bioactivity,
and
unique
chemical
structure.
This
review
provides
an
overview
of
different
marine-derived
inorganic
organic
materials,
such
calcium
carbonate,
magnesium
salts,
silica,
polysaccharides,
bioactive
peptides,
lipid-based
compounds,
effects
in
promoting
osteogenesis.
Specifically,
the
osteoinductive,
osteoconductive,
osteointegrative
activities
traditional
innovative
materials
that
influence
key
molecular
pathways
BMP/Smad
Wnt/β-catenin
signaling
underlying
bone
formation
will
be
evaluated.
also
prospects
approaches,
i.e.,
phage
display
technology,
optimize
peptides
targeted
regeneration.
In
context
sustainable
this
suggests
some
interesting
applications
unusual
able
overcome
limitations
conventional
ones
stimulate
cellular
by
activating
specific
pathways.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 10, 2025
Abstract
The
local
inflammatory
microenvironment,
insufficient
vascularization,
and
inadequate
bone
repair
materials
are
the
three
key
factors
that
constrain
of
defects.
Here,
we
synthesized
a
composite
nanoparticle,
TPQ
(TCP-PDA-QK),
with
core-shell
structure.
core
consists
nanotricalcium
phosphate
(TCP),
shell
is
derived
from
polydopamine
(PDA).
surface
modified
vascular
endothelial
growth
factor
(VEGF)
mimic
peptide
(QK
peptide).
was
then
embedded
in
porous
methacrylate
gelatin
(GelMA)
to
form
TPQGel
hydrogel.
In
environment,
hydrogel
can
gradually
release
drugs
through
pH
responsiveness,
promoting
M2
macrophage
polarization,
vascularization
regeneration
turn.
addition,
reprogrammed
macrophages
stimulate
generation
anti-inflammatory
pro-healing
factors,
which
provide
additional
support
for
angiogenesis
regeneration.
not
only
accurately
fill
irregular
defects
but
also
possesses
excellent
mechanical
properties
biocompatibility,
making
it
highly
suitable
minimally
invasive
treatment
Transcriptomic
tests
revealed
achieved
reprogramming
by
regulating
PI3K-AKT
signaling
pathway.
Overall,
be
harnessed
safe
efficient
therapeutics
accelerate
The FASEB Journal,
Journal Year:
2025,
Volume and Issue:
39(4)
Published: Feb. 25, 2025
Abstract
The
skeletal
system
comprises
closely
related
yet
functionally
distinct
bone
and
cartilage
tissues,
regulated
by
a
complex
network
of
transcriptional
factors
signaling
molecules.
Among
these,
core‐binding
factor
subunit
beta
(Cbfβ)
emerges
as
critical
co‐transcriptional
that
stabilizes
Runx
proteins,
playing
indispensable
roles
in
development
homeostasis.
Emerging
evidence
from
genetic
mouse
models
has
highlighted
the
essential
role
Cbfβ
directing
lineage
commitment
mesenchymal
stem
cells
(MSCs)
their
differentiation
into
osteoblasts
chondrocytes.
Notably,
deficiency
is
strongly
associated
with
severe
dysplasia,
affecting
both
endochondral
intramembranous
ossification
during
embryonic
postnatal
development.
In
this
review,
we
synthesize
recent
advancements
understanding
structural
molecular
functions
Cbfβ,
particular
focus
on
its
interactions
key
pathways,
including
BMP/TGF‐β,
Wnt/β‐catenin,
Hippo/YAP,
IHH/PTHrP.
These
pathways
converge
Cbfβ/RUNX2
complex,
which
orchestrates
gene
expression
program
for
osteogenesis,
formation,
integration
these
networks
ensures
precise
regulation
development,
remodeling,
repair.
Furthermore,
successful
local
delivery
to
address
abnormalities
underscores
potential
novel
therapeutic
target
disorders
such
cleidocranial
osteoarthritis,
metastases.
By
elucidating
mechanisms
underlying
function
insights
not
only
advance
our
biology
but
also
offer
promising
avenues
clinical
intervention,
ultimately
improving
outcomes
patients
disorders.
Biochimie,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 1, 2025
Skeletal
stem
cells
(SSCs)
reside
in
various
niche
locations
within
long
bones
to
maintain
bone
homeostasis
and
facilitate
fracture
repair.
Bone
fragility,
associated
with
ageing,
increases
the
susceptibility
of
femoral
head
fractures
due
an
increase
adipocytes
concomitant
loss
structural
integrity.
However,
specific
contribution
epiphyseal
SSCs
fragility
is
unknown.
To
explore
this,
a
comparative
analysis
was
performed
on
transcriptional
profiles
lineage
commitment
Wistar
rat
derived
from
marrow
(BM-),
diaphyseal
cortical
(CB-)
proximal
trabecular
(PF-SSCs)
isolated
same
bones.
were
characterized
based
morphology,
immunophenotype
(CD90/CD45),
growth
rate
(population
doubling
time),
gene
expression
differentiation
capacity
(Oil
Red
O,
Alizarin
S).
qRT-PCR
micro-arrays
evaluate
stemness,
SSC
lineage-specific
markers
both
undifferentiated
differentiated
states.
Our
findings
support
hypothesis
that
different
regions
exhibit
distinct
profiles,
reflecting
their
environments.
CB-SSCs
displayed
superior
osteogenic
potential
as
evidenced
by
key
genes
higher
levels
mineralization.
In
contrast,
PF-SSCs
had
reduced
adipogenic
potential.
Overall,
study
revealed
importance
niche-specific
cell
properties
for
use
regenerative
medicine
applications
provides
insight
into
role
risk.
Oncology Letters,
Journal Year:
2025,
Volume and Issue:
29(5), P. 1 - 12
Published: March 6, 2025
The
majority
of
cancer‑related
deaths
result
from
tumor
metastasis,
with
bone
metastasis
occurring
in
almost
all
types
malignant
tumors.
Understanding
the
mechanism
by
which
tumors
metastasize
to
is
critical
for
identification
novel
therapeutic
targets.
A
large
amount
research
has
been
carried
out
using
animal
models,
and
these
models
have
crucial
advancing
fundamental
understanding
cancer.
However,
current
are
limited;
although
they
can
mimic
specific
stages
metastatic
process,
not
able
replicate
entire
process
tumorigenesis
metastasis.
present
review
describes
molecular
changes
that
occur
intraosseous
microenvironment
metastases,
including
osteolytic
osteoblastic
types,
summarizes
advancements
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 28, 2025
Abstract
Bone
tissues
are
densely
innervated
by
nerve
fibers
throughout
the
periosteum
and
mineralized
bone.
The
impairment
of
bone
regeneration
is
a
critical
factor
contributing
to
challenges
in
osteoporotic
remodeling
repair.
Herein,
an
“ultrasound‐driven
regeneration”
strategy
proposed
additively
manufactured
degradable
Zn‐Cu
scaffolds.
vitro
investigations
with
RSC96
cells
elucidated
synergistic
promotion
low‐intensity
pulsed
ultrasound
(LIPUS)
metal
cations
on
Schwann
cell
proliferation
exosome
secretion.
Notably,
these
cell‐derived
exosomes,
once
internalized
neighboring
marrow
stromal
(BMSCs),
significantly
enhanced
their
migration,
osteogenic
differentiation,
extracellular
matrix
deposition,
indicating
potent
mechanism
for
regeneration.
Furthermore,
vivo
evaluation
validated
that
LIPUS
stimulation
activated
S100β‐positive
facilitated
peripheral
within
cranial
defects,
leading
accelerated
healing
rats
implantation
over
2‐
6‐week
recovery
periods.
This
work
provides
focusing
activation
enhancement
paracrine
effect,
especially
secretion,
which
further
recruited
surrounding
BMSCs
promoted
differentiation.
study
holds
considerable
promise
clinical
applications
translation
treatment
defects.
Stem Cell Research & Therapy,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: April 12, 2025
Mesenchymal
stem
cells
(MSCs)
play
a
crucial
role
in
bone
formation
and
remodeling.
Intrinsic
genetic
factors
extrinsic
environmental
cues
regulate
their
differentiation
into
osteoblasts.
Within
the
microenvironment,
complex
network
of
biochemical
biomechanical
signals
orchestrates
homeostasis
regeneration.
In
addition,
crosstalk
among
MSCs,
immune
cells,
neighboring
cells-mediated
by
extracellular
vesicles
non-coding
RNAs
(such
as
circular
micro
RNAs)
-profoundly
influences
osteogenic
Recent
studies
have
explored
specific
signaling
pathways
that
contribute
to
effective
regeneration,
highlighting
potential
manipulating
microenvironment
enhance
MSC
functionality.
The
integration
advanced
biomaterials,
gene
editing
techniques,
controlled
delivery
systems
is
paving
way
for
more
targeted
efficient
regenerative
therapies.
Furthermore,
artificial
intelligence
could
improve
tissue
engineering,
optimize
biomaterial
design,
enable
personalized
treatment
strategies.
This
review
explores
latest
advancements
emphasizing
intricate
interplay
molecules.
By
providing
comprehensive
overview
these
mechanisms
clinical
implications,
we
aim
shed
light
on
future
research
directions
this
rapidly
evolving
field.