Cloning andin silicoinvestigation of a putative voltage-gated calcium channel gene and protein inAstacus leptodactylus DOI Creative Commons
Berk Sağlam, Bora Ergin, Nazlı Coskun Beyatli

et al.

Turkish Journal of Biochemistry, Journal Year: 2023, Volume and Issue: 48(6), P. 675 - 681

Published: Nov. 14, 2023

Abstract Objectives Voltage-gated calcium channels are essential elements in development of many cellular processes like electrical signaling, contraction secretion and gene expression. There has been a fair amount information about the functional structural properties mammalian species. Crayfish serves as model animal for types experiments. However, there no related to molecular genetic crayfish. Methods Conventional cloning methods, three-dimensional calculations, docking experiments have conducted. Results An mRNA 7,791 bp size cloned. The coding region translated into an alpha peptide with 1,942 residues. cloned protein sequence similarity other L-type voltage-gated channel sequences from neighboring Three-dimensional structure, reference human channel, calculated. Known blockers, nifedipine, verapamil diltiazem successfully docked on calculated model. Conclusions Considering assay National Center Biotechnology Information (NCBI) platform, calculations it was concluded that codes putative In present work by using conventional biology methods complete de novo structure several pharmacological agents blocking identified receptor sites.

Language: Английский

Structural, Functional and Molecular Dynamics Examination of a de novo cloned Otopetrin-like Proton Channel in crayfish DOI
M. Erdem Sagsoz, Berk Sağlam, Kaan Arslan

et al.

Cell Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 82(3), P. 2029 - 2036

Published: May 29, 2024

Language: Английский

Citations

0
Cloning andin silicoinvestigation of a putative voltage-gated calcium channel gene and protein inAstacus leptodactylus DOI Creative Commons
Berk Sağlam, Bora Ergin, Nazlı Coskun Beyatli

et al.

Turkish Journal of Biochemistry, Journal Year: 2023, Volume and Issue: 48(6), P. 675 - 681

Published: Nov. 14, 2023

Abstract Objectives Voltage-gated calcium channels are essential elements in development of many cellular processes like electrical signaling, contraction secretion and gene expression. There has been a fair amount information about the functional structural properties mammalian species. Crayfish serves as model animal for types experiments. However, there no related to molecular genetic crayfish. Methods Conventional cloning methods, three-dimensional calculations, docking experiments have conducted. Results An mRNA 7,791 bp size cloned. The coding region translated into an alpha peptide with 1,942 residues. cloned protein sequence similarity other L-type voltage-gated channel sequences from neighboring Three-dimensional structure, reference human channel, calculated. Known blockers, nifedipine, verapamil diltiazem successfully docked on calculated model. Conclusions Considering assay National Center Biotechnology Information (NCBI) platform, calculations it was concluded that codes putative In present work by using conventional biology methods complete de novo structure several pharmacological agents blocking identified receptor sites.

Language: Английский

Citations

1