Molecular surveillance of resistance mutations in invasive populations of Spodoptera frugiperda in Europe, for evidence‐based pest control DOI Creative Commons
Konstantinos Mavridis, Vasiliki Evangelοu,

Alexandra M Grigoriadou

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Abstract BACKGROUND The invasive fall armyworm ( Spodoptera frugiperda , FAW), a highly destructive pest affecting more than 350 plant species, has recently invaded Europe raising urgent management concerns. Insecticide resistance profiling is essential to support evidence‐based control strategies. In this study, we analyzed target‐site insecticide mutations in FAW populations from Greece inform addition, DNA barcoding through cytochrome oxidase subunit 1 COI ) gene sequencing was used trace the pest's geographic origin and potential invasion pathways. RESULTS All specimens were identified as rice strain, exhibiting two almost balanced haplotypes (Haplotype 1: 58.6%; Haplotype 2: 41.4%), suggesting likely single, genetically diverse source population. Resistance‐associated ABCC2 (A > G single‐nucleotide polymorphism (SNP); up 80.9%) Ace‐1 (F290V: 37.5%; A201S: 3.85%), conferring Bacillus thuringiensis (Bt) organophosphates/carbamates, respectively. By contrast, no resistance‐associated detected for other key insecticides (diamides, pyrethroids, oxadiazines, spinosyns, avermectins), their current efficacy Greece. CONCLUSION This study provides critical baseline monitoring Europe, supporting development of sustainable integrated strategies line with European Union Green Deal. Continuous molecular diagnostics, alongside complementary bioassays, recommended mitigate impact on agriculture. © 2025 Author(s). Pest Management Science published by John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

SfMBP: A novel microbial binding protein and pattern recognition receptor in the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) DOI

Bang‐Xian Zhang,

Fangfang Liu,

Feng Liu

et al.

Developmental & Comparative Immunology, Journal Year: 2024, Volume and Issue: 154, P. 105142 - 105142

Published: Feb. 2, 2024

Language: Английский

Citations

4

Low concentrations of cyantraniliprole negatively affects the development of Spodoptera frugiperda by disruption of ecdysteroid biosynthesis and carbohydrate and lipid metabolism DOI

Shushu Lv,

Daojie Guan,

Jiaping Wei

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 200, P. 105827 - 105827

Published: Feb. 12, 2024

Language: Английский

Citations

4

Knockout of a testis‐specific gene cluster impairs male fertility in the fall armyworm, Spodoptera frugiperda DOI Open Access

Pei Wang,

Yuchen Zhao, Yalan Cheng

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

The function of some testis-specific genes (TSGs) in model insects have been studied, but their non-model remains largely unexplored. In the present study, we identified several TSGs fall armyworm (FAW), a significant agricultural pest, through comparative transcriptomic analysis. A gene cluster (TSGC) comprising multiple functional and long non-coding RNAs was found. Quantitative PCR analysis validated expression within this across different developmental stages. Interspecies collinearity indicated that TSGC is conserved among lepidopterans. To elucidate TSGC, employed CRISPR/Cas9 gene-editing technique to generate loss-of-function mutants. Notably, hatching rate eggs produced by wild-type females mated with mutant males (approximately 30%) substantially lower than from 80%). Additionally, reduction size number sperm observed testis compared males. Collectively, study not only elucidates role supporting male fertility FAW, which provides valuable information for understanding mechanism reproduction insects, also identifies potential target development genetic control strategies against lepidopteran pests. © 2025 Society Chemical Industry.

Language: Английский

Citations

0

Cypermethrin induced physiological and metabolic changes in susceptible and resistant populations of Spodoptera litura (Fabricius) DOI
Arushi Mahajan, Sanehdeep Kaur

Ecotoxicology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

Citations

0

Evaluation of GS‐omega/kappa‐Hxtx‐Hv1a and Bt toxins against Bt‐resistant and ‐susceptible strains of Helicoverpa zea (Boddie) and Spodoptera frugiperda (J.E. Smith) DOI Creative Commons

Simeon Ross,

Fei Yang, José C Santiago‐González

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Abstract BACKGROUND Helicoverpa zea and Spodoptera frugiperda are destructive insect pests of cotton corn. Both have evolved practical resistance to transgenic crops producing Bacillus thuringiensis ( Bt ) toxins in the United States. GS‐omega/kappa‐Hxtx‐Hv1a (ω/κ‐Hv1a) is a novel biopesticide derived from spider venom with activity against lepidopteran pests. The efficacy ω/κ‐Hv1a dependent on combining it facilitator product such as ‐based insecticide. However, unknown if can be an alternative for management resistance. In this study, we tested effect alone or presence susceptible resistant H. S. frugiperda. RESULTS Diet overlays 5 μg cm −2 Vip3Aa39 significantly increased mortality but did not enhance Vip3A‐resistant . contrast, addition 10 Cry1Ac surface incorporated diet Cry‐resistant compared diets without toxin. CONCLUSION study confirms that enhanced by mechanism. Our results suggest pore formation midgut membrane , enables ω/κ‐Hv1a. Vip3Aa involves altered toxin‐binding, consequently preventing Therefore, insecticides containing may managing help delay evolution © 2025 Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Citations

0

Advancements in Life Tables Applied to Integrated Pest Management with an Emphasis on Two-Sex Life Tables DOI Creative Commons

Zhenfu Chen,

Yang Luo,

Liang Wang

et al.

Insects, Journal Year: 2025, Volume and Issue: 16(3), P. 261 - 261

Published: March 3, 2025

Life tables are indispensable in IPM, offering an analysis of insect population dynamics. These record survival rates, fecundity, and other parameters at various developmental stages, enabling the identification key factors that affect numbers prediction growth trajectories. This review discusses application life agricultural pest management, including assessment control capacity natural enemies, evaluation biological agents, screening insect-resistant plant species. In vector control, used to evaluate transmission risks, model dynamics, interfere with cycles insects. For invasive pests, help us monitor dynamics predict future sizes. chemical assist evaluating fitness costs pesticide resistance, guiding insecticide selection, optimizing timing. final section, we explore research directions, emphasizing potential integrating new technologies such as genomics, ethology, satellite remote sensing enhance table improve IPM strategies.

Language: Английский

Citations

0

CRISPR/Cas: An emerging molecular technology for biological control of fall armyworm DOI Creative Commons
Ehab El‐Awaad, Hans Merzendorfer

New plant protection., Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

Abstract The fall armyworm, Spodoptera frugiperda , is a highly invasive pest that poses severe threats to food crop production worldwide. Several methods have been applied control armyworm infestations, including egg parasitoids, chemical insecticides, and transgenic crops expressing Bacillus thuringiensis (Bt) toxins. However, the currently available are either poorly effective or facing challenges such as resistance development environmental concerns. rapidly evolving molecular tools of genetic engineering, particularly clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated (Cas), increasingly utilized in recent years attempts develop novel management strategies genetically resistant crops. CRISPR/Cas‐based genome editing offers precise efficient tool has used successfully several functional genomic studies over last few years. In this review, we summarize progress using CRISPR/Cas for enabling targeted modification key genes essential survival propagation pest. potential system insect‐resistant approaches can be integrated into current discussed.

Language: Английский

Citations

0

Thermal performance drifts between the egg parasitoid Telenomus remus and the fall armyworm may threaten the efficacy of biological control under climate change DOI Creative Commons
Macdonald Mubayiwa, Honest Machekano, Brighton M. Mvumi

et al.

Entomologia Experimentalis et Applicata, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract The fall armyworm, Spodoptera frugiperda J.E. Smith, is a significant global agricultural pest known for its rapid invasion and devastating impact on crops. While pesticides may be effective controlling the in short‐term, they cause several socioeconomic ecological costs that highlight need more sustainable management strategies. Telenomus remus (Nixon) promising egg parasitoid biological control. For to provide ecosystem services, it should able survive coexist within same niches as host. However, there limited information regarding potential responses of T. thermal changes. In context changing climate environments, key understand parasitoid's overall environmental fitness relation We investigated effects short‐term (2 h) long‐term (6 acclimation pretreatment adults S. eggs high low temperatures (18°C 32°C, respectively) comparison with control (28°C). (critical maxima [CT max ], heat knockdown time critical minima min ]), parasitism rates, adult emergence were determined. Pretreated assessed hatchability under conditions. Acclimation at (18°C) (32°C) significantly reduced increased tolerance, respectively. Both temperatures, however, cold tolerance. tolerance polygons following pretreatment, showed heat‐ but not cold‐tolerance gains. Fall armyworm acclimated 32°C had higher ( p < 0.05) comparable treatment. Similarly, rates lower 0.001) host 32°C. These findings suggest decouple ‐fall relationship, threatening success warming regions. valuable insights into resilience suitability agent across different climates or geographies.

Language: Английский

Citations

0

Fall armyworm threatens Asian rice security: A review of sustainable management strategies DOI
Lekhnath Kafle,

Ravindra Chandra Joshi

CABI Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Abstract Rice ( Oryza sativa L.), a primary staple food for billions of people worldwide, faces growing threat from the invasive fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), in Asia. Given predominance small-scale farmers Asian rice production, which accounts over two-thirds global output, region is highly vulnerable to FAW infestations. The FAW’s rapid reproduction, extensive dispersal capabilities, and remarkable adaptability pose significant risks, with potential substantial yield losses comparable those observed other crops. Recent reports highlight concerning host shift corn-specialized strain towards cultivation Beyond direct losses, infestations can lead excessive use pesticides, posing serious threats human health environment safety. Effective sustainable management crucial ensuring long-term agricultural sustainability. Control strategies encompass diverse range approaches, including cultural techniques, biological methods, chemical interventions, habitat manipulation. absence varieties resistant underscores urgent need accelerated development through genetic engineering. Cultural control practices, such as staggered planting, deep tillage, crop rotation non-host species, improved field sanitation, effectively reduce populations. While promising, methods like trap cropping push-pull require further validation systems. Biological measures utilize natural predators, big-eyed bugs ground beetles, parasitoids Telenomus remus Campoletis sonorensis . Additionally, entomopathogenic agents Metarhizium rileyi , Bacillus thuringiensis (Bt), Beauveria bassiana offer environmentally alternatives insecticides. synthetic insecticides remain an essential component management, increasing prevalence pesticide resistance associated economic necessitate eco-friendly solutions. multiple insecticide classes robust strategies. This review emphasizes critical role integrated pest (IPM) combating Asia, advocating balanced approach that prioritises control, effective judicious insecticides, mitigate damage ensure security region.

Language: Английский

Citations

0

Time-series RNA sequencing reveals that immulectin-1 (IML-1) is a potential target for biological control of fall armyworm, Spodoptera frugiperda DOI
Fangfang Liu, Haiyan Ren,

Feng Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142466 - 142466

Published: March 1, 2025

Language: Английский

Citations

0