Gut bacteria in potato tuberworm (Phthorimaea operculella) populations are dominated by Enterococcus spp. and these play a significant role in carbohydrate metabolism and host growth

Journal of Pest Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Language: Английский

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Native mid-gut bacterial community increases resistance to nucleopolyhedrovirus in the cotton leafworm

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106462 - 106462

Published: May 1, 2025

Language: Английский

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Effects of different hosts and ages on the diversity of larval gut bacteria in Tuta absoluta

Entomologia Experimentalis et Applicata, Journal Year: 2025, Volume and Issue: unknown

Published: May 24, 2025

Abstract Gut bacterial composition is closely associated with the food intake and developmental age of herbivorous insects. In this study, we aimed to investigate diversity larval gut bacteria in different instar stages Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) feeding on hosts. DNA was extracted from 1st, 2nd, 3rd, 4th larvae T. tomatoes, potatoes, wolfberries for three generations. Subsequently, richness were analyzed via second‐generation Illumina MiSeq high‐throughput sequencing. Alpha index analysis revealed highest abundance fed wolfberry potato leaves, respectively. The observed 1st‐instar tomato leaves. leaves exhibited lowest stage 2nd‐instar stage. Proteobacteria dominant phylum 1–4 host plants. genus Enterobacter (60.1%) 4th‐instar tomatoes Wolbachia those other PICRUSt2 gene function prediction that played essential roles digestion nutrient supply. Specifically, may enhance nucleotide metabolism potatoes. Overall, study provides a basis explore interactions suggests directions its adaptive evolution integrated management.

Language: Английский

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Metagenomic profiling of gut microbiota in Fall Armyworm (Spodoptera frugiperda) larvae fed on different host plants

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 11, 2024

Language: Английский

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The immunostimulatory role of an Enterococcus-dominated gut microbiota in host protection against bacterial and fungal pathogens in Galleria mellonella larvae

Frontiers in Insect Science, Journal Year: 2023, Volume and Issue: 3

Published: Oct. 26, 2023

Understanding the intricate interplay between gut microbiota and immune response in insects is crucial, given its diverse impact on pathogenesis of various microbial species. The microbiota’s modulation host system one such mechanism, although complete responses remains elusive. This study investigated tripartite interaction microbiota, pathogens, host’s Galleria mellonella larvae reared under axenic (sterile) conventional (non-sterile) conditions. influence fitness during infections was evaluated via two different routes: oral infection induced by Bacillus thuringiensis subsp. galleriae ( Btg ), topical Metarhizium robertsii Mr ). We observed that without a can successfully fulfill their life cycle, albeit with more variation developmental time. subsequently performed survival assays final-instar larvae, using median lethal dose (LD 50 ) . Our findings indicated were vulnerable to an ; specifically, calculated be half-lethal for group resulted 90%–100% mortality rate group. Through dual-analysis experimental design, we could identify status 16S rRNA sequencing assess level immune-related gene expression same at basal conditions infection. analysis revealed our conventionally population dominated entirely four Enterococcus species, these species potentially stimulated gut, due increased antimicrobial peptides (AMPs)—gallerimycin gloverin—in compared larvae. Furthermore, mundtii , isolated from showed inhibition activity against vitro Lastly, other effectors, namely, phenoloxidase hemolymph total reactive oxygen/nitrogen (ROS/RNS) tested further investigate extent stimulation response. These highlight immune-modulatory role Enterococcus- increasingly reported assemblage laboratory populations Lepidoptera, infections.

Language: Английский

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Multi-omics reveals the ecological and biological functions of Enterococcus mundtii in the intestine of lepidopteran insects

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2024, Volume and Issue: 52, P. 101309 - 101309

Published: Aug. 10, 2024

Language: Английский

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Host Species Affects Gut Microbial Community and Offspring Developmental Performances in the Pupal Parasitoid Chouioia cunea Yang (Hymenoptera: Eulophidae)

Insects, Journal Year: 2024, Volume and Issue: 15(9), P. 722 - 722

Published: Sept. 20, 2024

Chouioia cunea are known to exploit in varying degrees a wide range of lepidopteran species and its offspring development may vary with host species. This study examined preimaginal larval gut microbiota parasitizing five folivorous hosts including Hyphantria (referred thereafter as CcHc), Antherea pernyi (CcAp), Helicoverpa armigera (CcHa), Spodoptera exigua (CcSe), frugiperda (CcSf). Though rates parasitism eclosion did not change species, the period number eclosed varied hosts, shortest CcSf highest from CcAp. For microbiota, though phylum Proteobacteria was dominant for attacking CcAp, Firmicutes so other hosts. All microbial genera except Enterococcus were less abundant than The database-based predictions indicate significant positive correlation between Cutibacterium Aureimonas relative wasp emergence, while Blastomonas exhibits strong association developmental period. Our results imply potential relevance community larvae attacked by C. cunea.

Language: Английский

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Seminal fluid proteins in the Queensland fruit fly: tissue origins, effects of mating and comparative genomics

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: unknown, P. 104247 - 104247

Published: Dec. 1, 2024

In many insect species, the ability of males to inhibit their mates from remating is an important component fitness. This also essential for effective management pests, including tephritid fruit flies, using Sterile Insect Technique. Here we apply transcriptomics and proteomics male reproductive tissues before after mating characterize components semen that might mediate inhibition in Queensland fly. We found 144 genes whose transcripts were enriched, or proteins expressed, tissue which varied amount mating. Some associated with testes, accessory glands ejaculatory apodeme, but those apodeme over-represented compared not enriched responsive. These included 13 related clustered within one Mb on chromosome 5. Functional annotations implicated a broad range biochemical processes genes/proteins responsive, cuticle structure most commonly among subset these apodeme-enriched kinase involved vitellogenesis genes. did find homolog much studied Drosophila melanogaster Sex Peptide comparative genomics indicated some tissue-enriched, responsive rapidly evolving tephritids (including fly lineage), suggesting recent adaptation new functional niches. Our results provide set candidate mediators further testing.

Language: Английский

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The synergistic potential of polyethylene glycol 400 for the control of Hyphantria cunea larvae by Beauveria bassiana

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 206, P. 106182 - 106182

Published: Oct. 25, 2024

Language: Английский

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