Gene co-expression analysis reveals conserved and distinct gene networks between resistant and susceptible Lens ervoides challenged by hemibiotrophic and necrotrophic pathogens DOI Creative Commons
Zhe Cao, Sabine Banniza

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 23, 2024

As field crops are likely to be challenged by multiple pathogens during their development, the investigation of broad-spectrum resistance in host is great interest for crop genetic enhancement. In this study, we attempted address question adopting a weighed gene co-expression approach study temporal transcriptome dynamics resistant and susceptible recombinant inbred lines (RILs) derived from an intraspecific Len ervoides cross infection process with either necrotrophic Ascochyta lentis or Stemphylium botryosum, hemibiotrophic pathogen Colletotrichum lentis. By comparing networks RILs, seven network module pairs were found possess high correlation coefficients (R > 0.70) large number overlapping genes (n 100). The conserved co-regulation these involved plant cell wall synthesis, division, cytoskeleton organization, protein ubiquitin related processes appeared common disease responses against pathogens. On other hand, also identified eight modules low between susceptibility networks. Among those, stronger R-genes small RNA hosts may enhancing L. A. lentis, C. S. whereas higher level synergistic regulation synthesis arginine glutamine phospholipid glycerophospholipids contribute increased ervoides.

Language: Английский

microRNAs: Key Regulators in Plant Responses to Abiotic and Biotic Stresses via Endogenous and Cross-Kingdom Mechanisms DOI Open Access
Tianze Ding,

Wenkang Li,

Fuguang Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1154 - 1154

Published: Jan. 18, 2024

Dramatic shifts in global climate have intensified abiotic and biotic stress faced by plants. Plant microRNAs (miRNAs)—20–24 nucleotide non-coding RNA molecules—form a key regulatory system of plant gene expression; playing crucial roles growth; development; defense against stress. Moreover, they participate cross-kingdom communication. This communication encompasses interactions with other plants, microorganisms, insect species, collectively exerting profound influence on the agronomic traits crops. article comprehensively reviews biosynthesis miRNAs explores their impact growth, development, resistance through endogenous, non-transboundary mechanisms. Furthermore, this review delves into effects pests. It proceeds to specifically discuss design modification strategies for artificial (amiRNAs), as well protection transport exosome-like nanovesicles (ELNVs), expanding potential applications crop breeding. Finally, current limitations associated harnessing are addressed, utilization synthetic biology is proposed facilitate heterologous expression large-scale production miRNAs. novel approach suggests plant-based solution address future biosafety concerns agriculture.

Language: Английский

Citations

11
A trade-off between investment in molecular defense repertoires and growth in plants DOI
Michael Giolai, Anna‐Liisa Laine

Science, Journal Year: 2024, Volume and Issue: 386(6722), P. 677 - 680

Published: Nov. 7, 2024

Given the negative fitness effects that pathogens impose on their hosts, benefits of resistance should be universal. However, there is marked variation across plant species in number nucleotide-binding leucine-rich repeat receptors, which form a cornerstone defense. The growth-defense trade-off hypothesis predicts costs associated with defense investment to generate these traits. Our analysis comparing features intracellular immune-receptor repertoires trait data 187 shows wild plants, size molecular repertoire correlates negatively growth. By contrast, we do not find evidence for agricultural plants. cross-species approach highlights central role shaping ecological and its sensitivity domestication.

Language: Английский

Citations

11
Classification of and detection techniques for RNAi-induced effects in GM plants DOI Creative Commons
Cecilia Díaz, Steve Ayobahan, Samson Simon

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

RNA interference (RNAi) is a biotechnological tool used for gene silencing in plants, with both endogenous and exogenous applications. Endogenous approaches, such as host-induced (HIGS), involve genetically modified (GM) while methods include spray-induced (SIGS). The RNAi mechanism hinges on the introduction of double-stranded (dsRNA), which processed into short interfering RNAs (siRNAs) that degrade specific messenger (mRNAs). However, unintended effects non-target organisms GM plants are concern due to sequence homologies or siRNA-induced epigenetic changes. Regulatory bodies EPA EFSA emphasize need comprehensive risk assessments. Detecting complex, often relying bioinformatic tools untargeted analyses like transcriptomics metabolomics, though these require extensive genomic data. This review aims classify mechanisms induced by from different sources identify technologies can be detect effects. In addition, practical case studies summarized discussed previously have been investigated. Current literature limited but suggests relatively specific, few observed crops. further needed fully understand mitigate potential risks, particularly those related transcriptional (TGS) mechanisms, less predictable than post-transcriptional (PTGS). Particularly application approaches small sequencing recommended thorough

Language: Английский

Citations

0
DCL2-dependent regulation of sRNA biogenesis and translocation in soybean DOI Creative Commons

Xiangying Kong,

Kun-Sheng Lyu,

Ruixin Xu

et al.

The Crop Journal, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
miR158a negatively regulates plant resistance to Phytophthora parasitica by repressing AtTN7 that requires EDS1‐PAD4‐ADR1 complex in Arabidopsis thaliana DOI
Yilin Li,

Xiuhong Gou,

R. Ma

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

SUMMARY Small RNAs are involved in diverse cellular processes, including plant immunity to pathogens. Here, we report that miR158a negatively regulates the oomycete pathogen Phytophthora parasitica Arabidopsis thaliana . By performing real‐time quantitative PCR, transient expression, and RNA ligase‐mediated 5′ rapid amplification of cDNA ends assays, demonstrate downregulates AtTN7 expression by cleaving its 3′‐untranslated region. positively affects encodes a truncated intracellular nucleotide‐binding site leucine‐rich repeat receptor containing Toll/interleukin‐1 receptor. can degrade oxidized forms nicotinamide adenine dinucleotide (NAD+). Further genetic molecular analyses reveal Enhanced Disease Susceptibility 1‐Phytoalexin Deficient 4‐Activated Resistance 1 complex is required for AtTN7‐mediated immunity. ADR1‐dependent Ca 2+ influx crucial activating salicylic acid signaling condition AtTN7‐triggered Our study uncovers immune roles regulatory mechanisms target Both miR158a‐downregulation ‐overexpression lead enhanced resistance P. without affecting growth phenotypes, suggesting their application potentials utilization miRNAs identifying novel genes development germplasm resources with disease resistance.

Language: Английский

Citations

1
Advances in plant molecular biology: towards new challenges DOI Creative Commons
Ángeles Aroca, Irene Garciݩa

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 74(19), P. 5949 - 5954

Published: Oct. 13, 2023

Language: Английский

Citations

2
Gene co-expression analysis reveals conserved and distinct gene networks between resistant and susceptible Lens ervoides challenged by hemibiotrophic and necrotrophic pathogens DOI Creative Commons
Zhe Cao, Sabine Banniza

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 23, 2024

As field crops are likely to be challenged by multiple pathogens during their development, the investigation of broad-spectrum resistance in host is great interest for crop genetic enhancement. In this study, we attempted address question adopting a weighed gene co-expression approach study temporal transcriptome dynamics resistant and susceptible recombinant inbred lines (RILs) derived from an intraspecific Len ervoides cross infection process with either necrotrophic Ascochyta lentis or Stemphylium botryosum, hemibiotrophic pathogen Colletotrichum lentis. By comparing networks RILs, seven network module pairs were found possess high correlation coefficients (R > 0.70) large number overlapping genes (n 100). The conserved co-regulation these involved plant cell wall synthesis, division, cytoskeleton organization, protein ubiquitin related processes appeared common disease responses against pathogens. On other hand, also identified eight modules low between susceptibility networks. Among those, stronger R-genes small RNA hosts may enhancing L. A. lentis, C. S. whereas higher level synergistic regulation synthesis arginine glutamine phospholipid glycerophospholipids contribute increased ervoides.

Language: Английский

Citations

0