Spatial distribution analysis of secondary metabolites in bolted and unbolted Saposhnikovia divaricata by atmospheric pressure laser ablation carbon fiber ionization mass spectrometry imaging

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 344096 - 344096

Published: April 1, 2025

Language: Английский

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Spatio-temporal plant hormonomics: from tissue to subcellular resolution

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(17), P. 5295 - 5311

Published: June 26, 2024

Abstract Due to technological advances in mass spectrometry, significant progress has been achieved recently plant hormone research. Nowadays, hormonomics is well established as a fully integrated scientific field focused on the analysis of phytohormones, mainly their isolation, identification, and spatiotemporal quantification plants. This review represents comprehensive meta-study phytohormone by spectrometry over past decade. To address current trends future perspectives, Web Science data were systematically collected key features such spectrometry-based analyses evaluated using multivariate methods. Our findings showed that currently divided into targeted untargeted approaches. Both aim miniaturize sample, allowing high-resolution be covered organs subcellular compartments. Therefore, we can study biosynthesis, metabolism, signalling at spatio-temporal resolution. Moreover, this trend accelerated fluorescence-activated cell sorting or imaging.

Language: Английский

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Identification of Plant Compounds with Mass Spectrometry Imaging (MSI)

Metabolites, Journal Year: 2024, Volume and Issue: 14(8), P. 419 - 419

Published: July 30, 2024

The presence and localization of plant metabolites are indicative physiological processes, e.g., under biotic abiotic stress conditions. Further, the chemical composition parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique exploring visualizing spatial distribution molecules within tissue. This review provides summary mass methods used mapping identifying in tissues. We present benefits disadvantages both vacuum ambient ionization methods, considering direct indirect approaches. Finally, we discuss current limitations annotating perspectives future investigations.

Language: Английский

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Stress Response of Citrus Leaves under Mechanical Damage and Huanglongbing Disease Infection Using Plasmonic TiO2 Nanotube Substrate-Based Imprinting Mass Spectrometry Imaging

Agronomy, Journal Year: 2024, Volume and Issue: 14(8), P. 1797 - 1797

Published: Aug. 15, 2024

Mapping the molecular signatures and metabolic regulation of plant tissues under biotic/abiotic stresses defensive responses has become a subject increasing interest in biology systems biology, but determining when where specialized metabolites are produced accumulated currently remains somewhat elusive goal. Herein, we demonstrated use TiO2 nanotube-based composite substrate modified with plasmonic gold nanoparticles hydrophobic polydopamine (AuNP-hPDA-TDNT) for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) analysis wide range pesticides visualizing stress-responsive citrus leaves during various defense processes. This method enabled visualization non-uniform tissue-specific distribution patterns functional that were mechanically damaged, fed to larvae, infected by Huanglongbing disease. Interestingly, some exhibited different accumulation mechanical damage larval feeding, suggesting plant-derived secondary exercise specific functions respect Moreover, early diagnosis detection HLB disease-associated biomarkers can facilitate prevention diseases. Overall, this imprinting MS imaging strategy will expand scope techniques providing more biologically relevant insights into biosynthesis, accumulation, role bioactive economically important plants.

Language: Английский

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Drug or Toxic? A Brief Understanding of the Edible Corolla of Rhododendron decorum Franch. by Bai Nationality with Comparative Metabolomics Analysis

Metabolites, Journal Year: 2024, Volume and Issue: 14(9), P. 484 - 484

Published: Sept. 4, 2024

is a traditional ornamental and medicinal plant in China, renowned for its aesthetic appeal therapeutic properties. Regarding

Language: Английский

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Metabolic strategies in hypoxic plants

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Complex multicellular organisms have evolved in an oxygen-enriched atmosphere. Oxygen is therefore essential for all aerobic organisms, including plants, energy production through cellular respiration. However, plants can experience hypoxia following extreme flooding events and also under aerated conditions proliferative organs or tissues characterized by high oxygen consumption. When availability compromised, adopt different strategies to cope with limited aeration. A common feature among plant species the activation of anaerobic fermentative metabolism provide ATP maintain homeostasis hypoxia. Fermentation requires many sugar substrates, which not always feasible, alternative metabolic are thus needed. Recent findings shown that hypoxic active specific conditions. Here, we describe regulatory mechanisms control how they enable them thrive despite challenging comprehensive mechanistic understanding genetic physiological components underlying should help opportunities improve resilience current climate change scenario.

Language: Английский

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Recent progress and ongoing challenges in Rhizoma atractylodis research: biogeography, biosynthesis, quality formation and control

Medicinal Plant Biology, Journal Year: 2023, Volume and Issue: 2(1), P. 0 - 0

Published: Jan. 1, 2023

Rhizoma atractylodis, including Atractylodes lancea and chinensis, is widely utilized worldwide as a Chinese meteria medica (CMM) due to its rich content of various sesquiterpenoids other medicinal components. Benefiting from the rapid ongoing advancements in analytical technologies, significant strides have been achieved advancing analysis atractylodis towards more systematic, comprehensive, personalized, intelligent approach. In this review, we highlight progress made biogeography, biosynthesis, quality formation, control over past decade. Special attention given incorporation advanced genetics tools, modern omics technology, chemometric methods that facilitate accurate utilization atractylodis. The elucidation biosynthetic pathways, formation quality, active components continue pose challenges study Additionally, review presents novel directions opportunities aimed at enhancing research efforts.

Language: Английский

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Matrix‐free laser desorption/ionization mass spectrometry imaging for rapid evaluation of wood biomass conversion

Rapid Communications in Mass Spectrometry, Journal Year: 2024, Volume and Issue: 38(14)

Published: May 13, 2024

Abstract Rationale This study overcomes traditional biomass analysis limitations by introducing a pioneering matrix‐free laser desorption/ionization (LDI) approach in mass spectrometry imaging (MSI) for efficient lignin evaluation wood. The innovative acetic acid–peracetic acid (APA) treatment significantly enhances detection, enabling high‐throughput, on‐site analysis. Methods Wood slices, softwood from conifer tree (Japanese cypress) and hardwood broadleaf beech), were analyzed using MSI with Fourier transform ion cyclotron resonance spectrometer. developed APA demonstrated effectiveness of biomass. Results Our technique successfully distinguishes between earlywood latewood enables the distinct visualization these other wood tissues, such as radial parenchyma. reveals significant contrasts MSI. It has identified intense ions β‐ O ‐4‐type lignin, specifically parenchyma hardwood, highlighting method's precision utility tissue Conclusions benefits LDI include reduced peak overlap, consistent sample quality, preservation natural properties, enhanced analytical accuracy, operational costs. is poised to become standard method rapid precise important applications environmental research sustainable resource management crucial effective diverse biomass, paving way towards sustainable, circular society.

Language: Английский

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“Omic tools” for investigation creative plant systens

Notes in Current Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 6, 2024

The result of the genotype/environment (G/E) interaction affects success implementation genetic program a plant biological system any level, from cell population to multicellular organism. During this interaction, absorbs trophic and energy resources, processes assimilates them. Under normal conditions, signal perception transduction occurs against background homeostasis regulated by genome. Genetic control is exercised at all stages growth development systems via differential gene expression. activity metabolism coordinated cooparated action ionome, proteome, metabolome, transcriptome. Direct cross connections between these aspects life are established developed constantly manifest themselves in form dynamic phenotypic effects structural formations enzyme chains. Disturbanses within individual disconnection them reveal differences stable, sensitive unstable forms. obtained information basis for experiments obtain forms with improved characteristics. A range tasks has been outlined direction, there have already significant developments. Comparison dynamics functioning creative variants level showed their original Changes determined interactions transgenes endogenous genes can positive/negative/combined characteristics new system. Comparative studies vital will provide about process communication both tissues use various combinations “omic tools” facilitate discovery promising candidates among regulatory genes, as well promoters. On other hand, be stimulus improving methods directions research.

Language: Английский

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Mass Spectrometry Imaging to map metabolites in plant-microbe interactions: grapevine as a case study

OENO One, Journal Year: 2024, Volume and Issue: 58(3)

Published: July 16, 2024

Every year, viticulture faces several disease outbreaks caused by pathogens with different life cycles and modes of action, due to climate changes, the appearance new is a concern for viticulture. To cope pathogenic microorganisms, plant must recognise invading agent arm itself an arsenal defence reactions, including accumulation antimicrobial metabolites.The modulation metabolites one first responses biotic factors. Their rapid synthesis can greatly contribute strengthening allowing it adapt, minimise pathogen colonisation survive. Despite scientific community's efforts characterise grapevine pathogens, molecules involved in control their specific localisation remain be deciphered.Mass Spectrometry Imaging (MSI) analytical techniques enable visualise map spatial distribution numerous biological within tissues better understanding biosynthesis, function.In this review, we explore applications MSI study plant/microbe interactions focusing on studies. This review opens doors gain comprehensive dynamics variations metabolite profiles organs, at developmental stages under various stress conditions. knowledge crucial elucidating role mechanisms, identifying regions high or low production, which targeted breeding improve resistance traits have impact productivity quality.

Language: Английский

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