Artificial seed technology modified for long-term preservation of Lemna aequinoctialis DOI Creative Commons
Hoang Thi Nhu Phuong,

Truong Quy Phung

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Duckweeds, the Lemnaceae family, are employed in a wide range of industries, including agriculture, animal feed, wastewater treatment, biofuel production, and human food. Duckweeds therefore also interest for researchers. However, because to their rapid growth development ease contamination by bacteria algae during storage process, duckweed samples require great deal time effort be maintained. In order overcome difficulties preserving scientific research, we developed an approach modification ‘artificial seed’ procedure. We found that encapsulation entire fronds Lemna aequinoctialis sodium alginate (3, 4, 5%) calcium chloride (150, 200, 250 mM) were appropriate maintaining them metabolically reduced state. After being stored two months, there no obvious variations between artificial seeds having endosperm composition ½ DN, water. kept with DN medium containing 150 mM CaCl2 3% recovered best. Following year storage, noticeable difference could seen treatments various compositionsThe optimal conditions aqueous 200 alginate. This procedure offers effective, cost-saving long-term preservation strategy may adapted future further species.

Language: Английский

Duckweed genomes and epigenomes underlie triploid hybridization and clonal reproduction DOI Creative Commons
Evan Ernst, Bradley W. Abramson, Kenneth Acosta

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

The Lemnaceae (duckweeds) are the world's smallest but fastest-growing flowering plants. Prolific clonal propagation facilitates continuous micro-cropping for plant-based protein and starch production holds tremendous promise sequestration of atmospheric CO2. Here, we present chromosomal assemblies, annotations, phylogenomic analysis Lemna genomes that uncover candidate genes responsible unique metabolic developmental traits family, such as anatomical reduction, adaxial stomata, lack stomatal closure, carbon via crystalline calcium oxalate. have selectively lost required RNA interference, including Argonaute reproductive isolation (the triploid block) haploid gamete formation. Triploid hybrids arise commonly among Lemna, found mutations in highly conserved meiotic crossover could support polyploid meiosis. Further, mapping centromeres by chromatin immunoprecipitation suggests their epigenetic origin despite divergence underlying tandem repeats centromeric retrotransposons. Syntenic comparisons with Wolffia Spirodela reveal diversification these genera coincided "Azolla event" mid-Eocene, during which aquatic macrophytes reduced high CO2 levels to those current ice age. Facile regeneration transgenic fronds from tissue culture, aided silencing, makes a powerful biotechnological platform, exemplified recent engineering high-oil outperforms oil-seed crops.

Language: Английский

Citations

2
A survey of duckweed species in Southern Italy provided first distribution records of the hybrid Lemna × mediterranea in nature DOI Creative Commons
Leone Ermes Romano, Luca Braglia, Maria Adelaide Iannelli

et al.

Perspectives in Plant Ecology Evolution and Systematics, Journal Year: 2025, Volume and Issue: unknown, P. 125863 - 125863

Published: March 1, 2025

Language: Английский

Citations

1
Duckweed protein: Extraction, modification, and potential application DOI

Nurul Shaeera Sulaiman,

Hana Mohd Zaini,

Wan Rosli Wan Ishak

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 463, P. 141544 - 141544

Published: Oct. 5, 2024

Language: Английский

Citations

4
Developments in Toxicity Testing with Duckweeds DOI Creative Commons
Paul Ziegler

Journal of Xenobiotics, Journal Year: 2025, Volume and Issue: 15(2), P. 48 - 48

Published: March 26, 2025

Duckweeds are a family of small floating macrophytes (the Lemnaceae) that inhabit quiet freshwaters worldwide. They have long been employed to determine toxicity higher plants in the aquatic environment, and standardized national international protocols developed for this purpose using two representative species. While these protocols, which assess growth leaf-like fronds tested duckweed, indeed suitable still frequently used detecting water-borne substances plant life, they cumbersome lengthy, endpoints rather than depict timelines, provide no information as mechanisms involved indicated toxicity. Progress has made downscaling, shortening improving assay procedures, use alternative duckweed species, explored. Biomarkers toxic effect determined concomitantly with testing itself, their potential assessment recently greatly expanded by transcriptomic, proteomic metabolomic techniques complemented FITR spectroscopy, transformation genotoxicity timescale testing. Improved modern biomarker analysis can help both better understand underlying facilitate identification unknown toxins.

Language: Английский

Citations

0
Encapsulated fronds technology modified for long-term preservation of Lemna aequinoctialis DOI
Hoang Thi Nhu Phuong,

Truong Quy Phung

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2025, Volume and Issue: 161(1)

Published: April 1, 2025

Language: Английский

Citations

0
Genome diversity and evolution of the duckweed section Alatae comprising diploids, polyploids, and interspecific hybrids DOI Creative Commons
Anton Stepanenko, Luca Braglia, Jörg Fuchs

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(2)

Published: April 1, 2025

SUMMARY The section Alatae of genus Lemna the monocotyledonous aquatic duckweed family (Lemnaceae) consists rather diverse accessions with unknown phylogeny and unclear taxonomic assignment. In contrast to other duckweeds, some accessions, in addition mainly vegetative propagation, produce readily flowers viable seeds. We analyzed genomic diversity phylogenetic relationship 52 accessions. For this purpose, we applied multiple molecular cytogenetic approaches, including plastid nuclear sequence polymorphisms, chromosome counting, genome size determination, situ hybridization combination geographic distribution. uncovered ploidy variation, recurrent hybridization, backcrosses between species their hybrids. latter successfully spread over three continents. results elucidate evolution explain difficult assignment distinct Our study might be an example for analogous studies resolve hitherto relationships among genera Wolffiella Wolffia .

Language: Английский

Citations

0
Towards sustainable food security: Exploring the potential of duckweed (Lemnaceae) in diversifying food systems DOI Creative Commons
Chigozie E. Ofoedu, Hayriye Bozkurt, Jenny C. Mortimer

et al.

Trends in Food Science & Technology, Journal Year: 2025, Volume and Issue: unknown, P. 105073 - 105073

Published: May 1, 2025

Language: Английский

Citations

0
The Developmental Cycle of Spirodela polyrhiza Turions: A Model for Turion-Based Duckweed Overwintering? DOI Creative Commons
Paul Ziegler

Plants, Journal Year: 2024, Volume and Issue: 13(21), P. 2993 - 2993

Published: Oct. 26, 2024

Duckweeds are widely distributed small, simply constructed aquatic higher plants (the Lemnaceae) found on quiet freshwater surfaces. Species inhabiting temperate climates may have to cope with long periods of severe cold during the winter season. Several duckweeds form compact resting structures from assimilatory fronds growing season that can bridge inhospitable conditions in a quiescent state. Of these, turions separate mother and overwinter water body bottom dormant They surface, germinate, sprout resume active growth upon warming spring. The largest duckweed, Spirodela polyrhiza, been intensively examined as ultrastructure, factors governing their formation release dormancy, signals driving germination sprouting accompanying starch degradation. Comparative transcriptomics revealing molecular features this developmental cycle. results illustrate an elegant sequence reactions ensures survival even winters by frost avoidance vegetative mode. Since little is known about other duckweed fronds, S. polyrhiza turion cycle cannot be considered representative general but serve reference for corresponding investigations.

Language: Английский

Citations

3
Duckweed: exploring its farm-to-fork potential for food production and biorefineries DOI Creative Commons

Anim Ujong,

Joncer Naibaho,

Soudabeh Ghalamara

et al.

Sustainable Food Technology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Duckweed is a promising and sustainable aquatic plant offering an eco-friendly alternative for synthesizing high-value bio-products has potential across food industries, pharmaceuticals, bioenergy production.

Language: Английский

Citations

2
Transgenerational Plasticity Enhances the Tolerance of Duckweed (Lemna minor) to Stress from Exudates of Microcystis aeruginosa DOI Open Access
Gengyun Li, Tiantian Zheng, Gang Wang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 13027 - 13027

Published: Dec. 4, 2024

Transgenerational plasticity (TGP) refers to the influence of ancestral environmental signals on offspring's traits across generations. While evidence TGP in plants is growing, its role plant adaptation over successive generations remains unclear, particularly floating facing fluctuating environments. Duckweed (

Language: Английский

Citations

1