Transcriptomics Uncovers Pathways Mediating Low-Nitrogen Stress Tolerance in Two Foxtail Millet Varieties DOI Creative Commons

Jirong Wu,

Lu Chen,

Zhenrong Yang

et al.

Agriculture, Journal Year: 2025, Volume and Issue: 15(6), P. 628 - 628

Published: March 16, 2025

Nitrogen crucially impacts foxtail millet (Setaria italica) growth and development. Uncovering low nitrogen (LN) tolerance genes mechanisms is vital for breeding high use efficiency varieties. In this study, the LN of 50 genotypes was assessed through field trials seedling hydroponic experiments. Subsequently, transcriptome analysis performed on one highly sensitive genotype, named Maotigu, tolerant Dahuanggu, under (0.1 mmol/L) control (5 conditions in Compared to treatment, 823 differentially expressed (DEGs) (350 upregulated, 473 downregulated) were identified roots while 2427 DEGs (1703 724 detected Maotigu treatment. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment revealed that a total 3134 associated with pathways including plant–pathogen interaction, amino sugar nucleotide metabolism, others. A 116 commonly between Dahuanggu involving like galactose flavone flavonol biosynthesis. The 28 showed opposite expression patterns Maotigu; 18 further validated using qRT-PCR. These offer valuable insights into molecular underlying stress responses millet.

Language: Английский

Transcriptomics Uncovers Pathways Mediating Low-Nitrogen Stress Tolerance in Two Foxtail Millet Varieties DOI Creative Commons

Jirong Wu,

Lu Chen,

Zhenrong Yang

et al.

Agriculture, Journal Year: 2025, Volume and Issue: 15(6), P. 628 - 628

Published: March 16, 2025

Nitrogen crucially impacts foxtail millet (Setaria italica) growth and development. Uncovering low nitrogen (LN) tolerance genes mechanisms is vital for breeding high use efficiency varieties. In this study, the LN of 50 genotypes was assessed through field trials seedling hydroponic experiments. Subsequently, transcriptome analysis performed on one highly sensitive genotype, named Maotigu, tolerant Dahuanggu, under (0.1 mmol/L) control (5 conditions in Compared to treatment, 823 differentially expressed (DEGs) (350 upregulated, 473 downregulated) were identified roots while 2427 DEGs (1703 724 detected Maotigu treatment. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment revealed that a total 3134 associated with pathways including plant–pathogen interaction, amino sugar nucleotide metabolism, others. A 116 commonly between Dahuanggu involving like galactose flavone flavonol biosynthesis. The 28 showed opposite expression patterns Maotigu; 18 further validated using qRT-PCR. These offer valuable insights into molecular underlying stress responses millet.

Language: Английский

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