Comparative Transcriptomic Analysis and Candidate Gene Identification for Wild Rice (GZW) and Cultivated Rice (R998) Under Low-Temperature Stress DOI Open Access
Yongmei Yu, Dilin Liu, Wang Feng

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13380 - 13380

Published: Dec. 13, 2024

Rice is a short-day thermophilic crop that originated from the low latitudes of tropics and subtropics; it requires high temperatures for growth but sensitive to temperatures. Therefore, highly important explore analyze molecular mechanism cold tolerance in rice expand planting areas. Here, we report phenotypic evaluation based on low-temperature stress indica (R998) wild (GZW) comparative transcriptomic study conducted at six time points. After 7 days treatment 10 °C, R998 exhibited obvious yellowing greening leaves, while GZW resistance, leaves maintained their normal morphology no yellowing; has higher survival rate. Principal component analysis (PCA) cluster RNA-seq data revealed difference resistance between two cultivars was caused mainly by after 6 h. Differential expression 2615 unique differentially expressed genes (DEGs) material, 1578 DEGs 1874 GZW, 2699 were not only also different points same material under treatment. A total 15,712 detected significantly enriched phenylalanine metabolism, photosynthesis, plant hormone signal transduction, starch sucrose metabolism pathways. These included 1937 encoding transcription factors (TFs), which have been identified with functional validation previous studies. In addition, gene regulatory network constructed via weighted correlation (WGCNA), 12 key related identified, including five TFs, one verified results provide theoretical basis an in-depth understanding new genetic resources rice.

Language: Английский

A Series of Novel Alleles of Ehd2 Modulating Heading and Salt Tolerance in Rice DOI Creative Commons
Peng Xu,

Shulei Hao,

Xiaoxia Wen

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 297 - 297

Published: Jan. 20, 2025

Rice (Oryza sativa L.) is a staple crop for nearly half of the global population and one China’s most extensively cultivated cereals. Heading date, critical agronomic trait, determines regional seasonal adaptability rice varieties. In this study, series mutants (elh5 to elh12) exhibiting extremely late heading under both long-day (LD) short-day (SD) conditions were identified from an ethyl methanesulfonate (EMS) mutant library. Using MutMap map-based cloning, causative gene was as novel allele Ehd2/OsID1/RID1/Ghd10. Functional validation through CRISPR/Cas9 knockout complementation assays confirmed its role in regulating heading. The elh6 mutation found cause intron retention due alternative splicing. Ehd2 encodes Cys-2/His-2-type zinc finger transcription factor with IDD domain transcriptional activity yeast. Its expression peaks developing leaves before spikes during reproductive conversion. mutants, delayed resulted downregulating Ehd1-Hd3a pathway genes. Salinity stress significantly hampers growth productivity. Transcriptomic analysis elh10 ZH8015 seedlings exposed salt 24 h 5150 differentially expressed genes (DEGs) at seedling stage, predominantly linked response pathways. revealed modulator tolerance, likely regulation ion transport, enzyme activity, antioxidant systems. This study establishes pivotal promoting while negatively tolerance rice.

Language: Английский

Citations

0
An extensin-like gene OsPEX1 plays key roles in temperature stress response in rice DOI
Jieni Li, Ao Li, Yue Wang

et al.

Plant Cell Tissue and Organ Culture (PCTOC), Journal Year: 2025, Volume and Issue: 161(1)

Published: March 21, 2025

Language: Английский

Citations

0
Epigenetic regulation of ISPL10 enhances regional adaptability of rice varieties DOI

Zhiwen Yu,

Xiaoche Wang,

Yiran Wang

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

Suppressing the heading date of rice under short-day (SD) conditions while promoting it long-day (LD) can significantly enhance regional adaptability varieties. However, germplasm resources with these traits are scarce. In this study, we report Jumonji C (jmjC) protein-encoding gene ISPL10. The ispl10 mutant exhibited 7 days later SD and 14 earlier LD compared wild type (WT). ISPL10 decreased H3 lysine 9 dimethylation (H3K9me2) levels at OsMADS51 locus activated expression OsMADS51, which then enhanced Ehd1 up-regulated Hd3a conditions. By contrast, is directly bound to promoter OsVIL2 suppress its expression, thereby inhibiting reducing RFT1 Additionally, interacted Se14, another jmjC protein that controlled H3K4me3 states in chromatin. field tests showed not only extended growth period low-latitude regions but also shortened maturity duration high-latitude regions, thus increased grain yield both low- WT. Therefore, could be a crucial factor improving

Language: Английский

Citations

0
Flowering Newsletter 2024 DOI Creative Commons
Rainer Melzer

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(18), P. 5457 - 5458

Published: Aug. 9, 2024

Why do we love flowers?Why should everyone flowers?The answer depends on whom you ask-and when ask.Ask a freshly married couple Valentine's day and it is clear they flowers for their beauty, vibrant colours, captivating scent.Flowers as symbol of love.Yet, there poignant irony in giving cut flowers, which are already destined to die without fulfilling evolutionary purpose-to set seeds produce fruits.Which brings us the second possible why flowers.Ask botanically inclined divorced fruits products that bring consolation difficult times.The barley grains alcoholic beverages.The cocoa beans chocolate.

Language: Английский

Citations

1
Comparative Transcriptomic Analysis and Candidate Gene Identification for Wild Rice (GZW) and Cultivated Rice (R998) Under Low-Temperature Stress DOI Open Access
Yongmei Yu, Dilin Liu, Wang Feng

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13380 - 13380

Published: Dec. 13, 2024

Rice is a short-day thermophilic crop that originated from the low latitudes of tropics and subtropics; it requires high temperatures for growth but sensitive to temperatures. Therefore, highly important explore analyze molecular mechanism cold tolerance in rice expand planting areas. Here, we report phenotypic evaluation based on low-temperature stress indica (R998) wild (GZW) comparative transcriptomic study conducted at six time points. After 7 days treatment 10 °C, R998 exhibited obvious yellowing greening leaves, while GZW resistance, leaves maintained their normal morphology no yellowing; has higher survival rate. Principal component analysis (PCA) cluster RNA-seq data revealed difference resistance between two cultivars was caused mainly by after 6 h. Differential expression 2615 unique differentially expressed genes (DEGs) material, 1578 DEGs 1874 GZW, 2699 were not only also different points same material under treatment. A total 15,712 detected significantly enriched phenylalanine metabolism, photosynthesis, plant hormone signal transduction, starch sucrose metabolism pathways. These included 1937 encoding transcription factors (TFs), which have been identified with functional validation previous studies. In addition, gene regulatory network constructed via weighted correlation (WGCNA), 12 key related identified, including five TFs, one verified results provide theoretical basis an in-depth understanding new genetic resources rice.

Language: Английский

Citations

0