Integrating genomic sequencing resources: an innovative perspective on recycling with universal Angiosperms353 probe sets DOI Creative Commons
Xueqin Wang, Tao Xiong, Yiying Wang

et al.

Horticulture Advances, Journal Year: 2024, Volume and Issue: 2(1)

Published: Jan. 29, 2024

Abstract Sequencing strategies have continually advanced, with high-throughput sequencing (HTS) technologies emerging as pivotal tools in plant phylogenomics. As a standard form of target capture sequencing, hybridization enrichment (Hyb-seq) is innovative and flexible, then HTS strategy widely adopted The universal Angiosperms353 probe sets (Angiosperms353) are instrumental Hyb-seq been witnessed increased application recent years. This review integrates numerous publications empirical datasets to comprehensively assess the potential applications Angiosperms353. Through evaluations using 18-taxa 343-taxa genomic datasets, this explores factors influencing gene capture. RNA-seq yielded highest number genes, followed by whole-genome genome skimming. Increased depth enhanced yields, no evident bias was observed across all tested angiosperm groups. In genomics era extensive data, provides comprehensive guidelines for practical Angiosperms353, promoting resource recycling maximizing data sharing utilization.

Language: Английский

PhyloForge: Unifying Micro‐ and Macroevolution With Comprehensive Genomic Signals DOI
Ya Wang, Wei Dong,

Yufan Liang

et al.

Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

The dimensions of phylogenetic research have expanded to encompass the study large-scale populations at microevolutionary level and comparisons between different species or taxonomic units macroevolutionary level. Traditional tools often struggle handle diverse complex data required for these evolutionary scales. In response this challenge, we introduce PhyloForge, a robust tool designed seamlessly integrate demands both micro- macroevolution, comprehensively utilising phylogenomic signals, such as genes, SNPs, structural variations, well mitochondrial chloroplast genomes. PhyloForge's innovation lies in its capability multiple enabling unified analysis multidimensional genomic data. This unique feature empowers researchers gain more comprehensive understanding aspects biological evolution. PhyloForge not only provides highly customisable experienced but also features an intuitively interface, facilitating effortless beginners. Extensive testing across various domains, including animals, plants fungi, attests broad applicability field phylogenetics. summary, has significant potential era genomics, offering new perspective toolset deeper evolution life. codes could be found GitHub (https://github.com/wangyayaya/PhyloForge/), program installed Conda (https://anaconda.org/wangxiaobei/phyloforge).

Language: Английский

Citations

2

A new infrageneric classification of Gastrochilus (Orchidaceae: Epidendroideae) based on molecular and morphological data DOI Creative Commons
Junyi Zhang, Yue-Hong Cheng, Min Liao

et al.

Plant Diversity, Journal Year: 2023, Volume and Issue: 46(4), P. 435 - 447

Published: Aug. 14, 2023

is an orchid genus containing 73 species of mainly epiphytic on trees or rocks in mountain forests tropical and subtropical Asia. Previous phylogenetic analyses morphological assessments have failed to produce a well-resolved phylogeny at the infrageneric level. In present study, new classification

Language: Английский

Citations

6

GeneMiner: a tool for extracting phylogenetic markers from next-generation sequencing data DOI Open Access
Pulin Xie,

Yongling Guo,

Wenbin Zhou

et al.

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: April 17, 2023

The advancement of Next-generation Sequencing (NGS) technologies has led to a revolution in the field evolutionary biology. With increasing number available genomes and transcriptomes, researchers can mine various types molecular markers that are vital for phylogenetic, evolutionary, ecological studies. Numerous tools have been developed extract these from NGS data. However, due limited well-annotated reference non-model organisms, it is still challenging obtain accurately efficiently. Here, we present GeneMiner, an improved expanded version our previous tool, Easy353. GeneMiner involves reference-guided de Bruijn Graph assembly with seed self-discovery greedy extension. Additionally, includes verification step using parameter-bootstrap method reduce negative impact on caused by relatively distant reference. Our results both experimental simulation data showed acquire phylogenetic plants transcriptomic, genomic, other designed be user-friendly, fast, memory-efficient, compatible Linux, Windows, macOS. All source codes publicly GitHub easy accessibility transparency (https://github.com/happywithxpl/GeneMiner).

Language: Английский

Citations

4

Oreocharis oriolus, a new species of Gesneriaceae in a sclerophyllous oak community from Yunnan, Southwest China DOI Creative Commons
Jun Hu, Junyi Zhang, Hai He

et al.

Ecology and Evolution, Journal Year: 2023, Volume and Issue: 13(6)

Published: June 1, 2023

, a new species of Gesneriaceae in sclerophyllous oak community from Yunnan, Southwest China, is described and illustrated. Morphologically, it resembles both

Language: Английский

Citations

4

Integrating genomic sequencing resources: an innovative perspective on recycling with universal Angiosperms353 probe sets DOI Creative Commons
Xueqin Wang, Tao Xiong, Yiying Wang

et al.

Horticulture Advances, Journal Year: 2024, Volume and Issue: 2(1)

Published: Jan. 29, 2024

Abstract Sequencing strategies have continually advanced, with high-throughput sequencing (HTS) technologies emerging as pivotal tools in plant phylogenomics. As a standard form of target capture sequencing, hybridization enrichment (Hyb-seq) is innovative and flexible, then HTS strategy widely adopted The universal Angiosperms353 probe sets (Angiosperms353) are instrumental Hyb-seq been witnessed increased application recent years. This review integrates numerous publications empirical datasets to comprehensively assess the potential applications Angiosperms353. Through evaluations using 18-taxa 343-taxa genomic datasets, this explores factors influencing gene capture. RNA-seq yielded highest number genes, followed by whole-genome genome skimming. Increased depth enhanced yields, no evident bias was observed across all tested angiosperm groups. In genomics era extensive data, provides comprehensive guidelines for practical Angiosperms353, promoting resource recycling maximizing data sharing utilization.

Language: Английский

Citations

1