The core microbiota across the green lineage DOI Creative Commons
Paloma Durán

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 77, P. 102487 - 102487

Published: Dec. 5, 2023

The study of plant–microbe interactions and the characterization plant-associated microbiota has been focus plant researchers in last decades due to its importance for health natural conditions. Here, I explore persistent core associated with different species across environments by performing a meta-analysis publicly available datasets. Intra-specific analyses revealed that diverse genotypes growing similar habitats interact common set microbial groups but some these are species- or environment-specific. Furthermore, interspecific demonstrates conservation seven bacterial orders photosynthetic organisms, including microalgae, suggesting conserved capacity interaction microbes throughout evolutionary history. However, specific functions members whether hosts remain largely unexplored. therefore discuss understanding roles propose future research directions, exploration kingdoms. By investigating functions, it will be possible leverage this knowledge sustainable agricultural management goals.

Language: Английский

Continental-scale associations of Arabidopsis thaliana phyllosphere members with host genotype and drought DOI Creative Commons
Talia L. Karasov, Manuela Neumann, Laura Leventhal

et al.

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(10), P. 2748 - 2758

Published: Sept. 6, 2024

Abstract Plants are colonized by distinct pathogenic and commensal microbiomes across different regions of the globe, but factors driving their geographic variation largely unknown. Here, using 16S ribosomal DNA shotgun sequencing, we characterized associations Arabidopsis thaliana leaf microbiome with host genetics climate variables from 267 populations in species’ native range Europe. Comparing distribution 575 major bacterial amplicon variants (phylotypes), discovered that composition A. segregates along a latitudinal gradient. The clines predicted metrics drought, also spatial host. To validate relative effects drought genotype conducted common garden field study, finding 10% core bacteria to be affected directly 20% genetic drought. These data provide valuable resource for plant field, identified suggesting can indirectly shape via microbiome.

Language: Английский

Citations

1
Microbe-associated molecular pattern recognition receptors have little effect on endophytic Arabidopsis thaliana microbiome assembly in the field DOI Creative Commons

Caroline Oldstone-Jackson,

Feng Huang, Joy Bergelson

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 8, 2023

Plant microbiome structure affects plant health and productivity. A limited subset of environmental microbes successfully establishes within tissues, but the forces underlying this selectivity remain poorly characterized. Transmembrane pattern recognition receptors (PRRs), used by plants to detect microbe-associated molecular patterns (MAMPs), are strong candidates for achieving because PRRs can potentially interact with many members microbiome. Indeed, MAMPs found in microbial taxa, including beneficials commensals, instigate a robust immune response that growth. Surprisingly, we MAMP-detecting have little effect on endophytic bacterial fungal field. We compared microbiomes four PRR knockout lines Arabidopsis thaliana wild-type multiple tissue types over several developmental stages detected only subtle shifts fungal, not bacterial, β -diversity one mutants. In stage, lore mutants had slightly altered -diversity, indicating LORE may be involved plant-fungal interactions addition its known role detecting certain lipids. No other effects α variability, within-individual homogeneity, or load were found. The general lack suggests individual critical shaping Rather, suggest must either act concert and/or individually maintained through pleiotropic coevolved mutualists pathogens. Although unexpected, these results offer insights into plant-microbe help direct future efforts uncover host genetic elements control assembly.

Language: Английский

Citations

3
The genetic architecture of the adaptive potential of Arabidopsis thaliana in response to Pseudomonas syringae strains isolated from south‐west France DOI
Claudia Bartoli,

Mylène Rigal,

Carine Huard‐Chauveau

et al.

Plant Pathology, Journal Year: 2023, Volume and Issue: 73(4), P. 884 - 897

Published: Dec. 15, 2023

Abstract Phytopathogens are a threat for global food production and security. Emergence or re‐emergence of plant pathogens is highly dependent on the environmental conditions affecting pathogen spread survival. Under climate change, geographic expansion distribution poleward has been observed, potentially resulting in disease outbreaks crops wild plants. Therefore, estimating adaptive potential plants to novel epidemics describing underlying genetic architecture primary need propose agricultural management strategies reducing breed cultivars adapted that might under change. To address this challenge, we inoculated Pseudomonas syringae strains isolated from Arabidopsis thaliana populations south‐west France genetically polymorphic TOU‐A A . population north‐east France. While no was identified response most P strains, displayed variable JACO‐CL strain belonging phylogroup 7 (PG7). This carried reduced type III secretion system (T3SS) characteristic PG7 as well flexible genomic traits effectors. Genome‐wide association mapping 192 accessions revealed polygenic JACO‐CL. The main quantitative trait locus (QTL) region encompasses two R genes AT5G18310 gene encoding ubiquitin hydrolase, target AvrRpt2 effector. Altogether, our results pave way better understanding molecular basis an ecologically relevant – pathosystem.

Language: Английский

Citations

2
Biotic interactions promote local adaptation to soil in plants DOI Creative Commons
Thomas Dorey, Léa Frachon, Loren H. Rieseberg

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Abstract Although different ecological factors shape adaptative evolution in natural habitats, we know little about how their interactions impact local adaptation. Here used eight generations of experimental with outcrossing Brassica rapa plants as a model system, treatment groups that varied soil type, herbivory (with/without aphids), and pollination mode (hand- or bumblebee-pollination), to study biotic affect adaptation soil. First, show several plant traits evolved response soil-specific way. Second, using reciprocal transplant experiment, demonstrate significant soil-type the “number open flowers”, trait fitness proxy, but only bee pollination. Whole genome re-sequencing lines revealed caused 10-fold increase number SNPs across allele frequency change, alleles opposite change types (antagonistic pleiotropy) were most common an evolutionary history Our results interaction mutualists antagonists can facilitate type through antagonistic pleiotropy.

Language: Английский

Citations

0
The relative effects of abiotic and biotic factors in explaining the structure of soil bacterial communities at diverse taxonomic levels DOI Creative Commons
Baptiste Mayjonade,

Rémy Zamar,

Sébastien Carrère

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

ABSTRACT Soil microbes play pivotal roles in the multifunctioning of terrestrial ecosystems. In context global changes, there is an urgent need to protect soil microbial diversity, which relies on determining abiotic and biotic factors that influence composition, assemblage microbiota. A large number informative studies have reported edaphic properties climate as key drivers bacteria However, these were mainly conducted at phylum level based a restricted non-microbial variables. this study, we aimed estimate relative effects shaping bacterial communities diverse taxonomic levels by focusing 160 natural sites located southwest France for unique set variables available. After characterizing with highly taxonomically resolving gyrB gene, identified addition pH, temperature, precipitations, lowest appear strongly structured micronutrients, notably manganese. On other hand, highest interplay between descriptors plant properties. Similar previous observations pathogens, strong positive associations species presence particular suggest host specificity commensal bacteria. Altogether, deeper characterization both could help fuel programs designed protecting restoring ecosystem functions.

Language: Английский

Citations

0
Microbial communities living inside plant leaves or on the leaf surface are differently shaped by environmental cues DOI
Maryam Mahmoudi, Juliana Almario, Katrina Lutap

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Leaf-associated microbial communities can promote plant health and resistance to biotic abiotic stresses. However, the importance of environmental cues in assembly leaf endo- epi-microbiota remains elusive. Here we aimed investigate impact seasonal variations, on establishment microbiome, focusing long-term changes (five years) bacterial, fungal, non-fungal eukaryotic colonizing surface endosphere six wild Arabidopsis thaliana populations. While leaf-microbial were found be highly stochastic, niche had a predominant with endophytic consistently exhibiting lower diversity variability. Furthermore, our analyses revealed that among factors, radiation humidity-related factors are most important drivers paderns leaf, albeit stronger effects epiphytic communities. Using linear models, further identified 30 genera whose relative abundance compartments could modeled from variables, suggesting specific preferences for these taxa. With hypothesis interactions within communities, analyzed interaction networks across compartments. We showed more complex than endophytic, complexity connectivity partially correlated mentioned cues. Our results indicate humidity solar function as major shaping phyllosphere microbiome at micro-scale (leaf compartment) macro-scale (site). These findings have practical implications selecting developing field-adapted microbes face of, predicting invasions response global change.

Language: Английский

Citations

1
The core microbiota across the green lineage DOI Creative Commons
Paloma Durán

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 77, P. 102487 - 102487

Published: Dec. 5, 2023

The study of plant–microbe interactions and the characterization plant-associated microbiota has been focus plant researchers in last decades due to its importance for health natural conditions. Here, I explore persistent core associated with different species across environments by performing a meta-analysis publicly available datasets. Intra-specific analyses revealed that diverse genotypes growing similar habitats interact common set microbial groups but some these are species- or environment-specific. Furthermore, interspecific demonstrates conservation seven bacterial orders photosynthetic organisms, including microalgae, suggesting conserved capacity interaction microbes throughout evolutionary history. However, specific functions members whether hosts remain largely unexplored. therefore discuss understanding roles propose future research directions, exploration kingdoms. By investigating functions, it will be possible leverage this knowledge sustainable agricultural management goals.

Language: Английский

Citations

0