Hybridization between Aedes aegypti and Aedes mascarensis mosquitoes leads to disruption of male sex determination DOI Creative Commons
Jiangtao Liang, Lin Kang,

Pawel Michalak

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: July 22, 2024

Abstract Understanding the sex determination pathway and its disruptions in mosquitoes is critical for effective control of disease vectors through genetic manipulations based on separation. When male hybrids Aedes aegypti females Ae. mascarensis males are backcrossed to females, a portion backcross progeny manifests as with abnormal sexual differentiation. We discovered significant correlation between pupal abnormalities feminization subsequent adults exemplified by relative abundance ovarian testicular tissues. All intersex individuals were they expressed determining factor, Nix . Further, our analysis sex-specific splicing doublesex fruitless transcripts demonstrated presence both female splice variants indicating that disrupted. A comparative transcriptomic revealed similar expression levels most female-associated genes reproductive organs carcasses intersexual normal females. Moreover, intersexes had largely gene testes but downregulation accessory glands when compared males. conclude evolving hybrid incompatibilities involve disruption accompanied changes associated

Language: Английский

The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes DOI Creative Commons
Sergei Ryazansky, Chujia Chen, Mark Potters

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 25, 2024

Abstract Background Understanding genome organization and evolution is important for species involved in transmission of human diseases, such as mosquitoes. Anophelinae Culicinae subfamilies mosquitoes show striking differences sizes, sex chromosome arrangements, behavior, ability to transmit pathogens. However, the genomic basis these not fully understood. Methods In this study, we used a combination advanced technologies Oxford Nanopore Technology sequencing, Hi-C scaffolding, Bionano, cytogenetic mapping develop an improved chromosome-scale assembly West Nile vector Culex quinquefasciatus . Results We then annotate odorant receptors, binding proteins, transposable elements. A region containing male-specific sequences on 1 polymorphic inversion 3 were identified Cx. genome. addition, was compared with genomes other malaria vectors An. coluzzi albimanus , arboviruses Ae. aegypti Our work confirms significant expansion two chemosensory gene families well increase relocation elements both relative Anophelines. Phylogenetic analysis clarifies divergence time between mosquito species. study provides new insights into chromosomal finds that X sex-determining have significantly higher rate than autosomes. Conclusion The uncovered details has potential speed up development novel control strategies.

Language: Английский

Citations

11
Mosquito sex determination: recent advances and applications DOI

Melanie Hempel,

Joseph D. Warren, Jiangtao Liang

et al.

Current Opinion in Insect Science, Journal Year: 2025, Volume and Issue: unknown, P. 101385 - 101385

Published: May 1, 2025

Language: Английский

Citations

0
Hybridization between Aedes aegypti and Aedes mascarensis mosquitoes leads to disruption of male sex determination DOI Creative Commons
Jiangtao Liang, Lin Kang,

Pawel Michalak

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: July 22, 2024

Abstract Understanding the sex determination pathway and its disruptions in mosquitoes is critical for effective control of disease vectors through genetic manipulations based on separation. When male hybrids Aedes aegypti females Ae. mascarensis males are backcrossed to females, a portion backcross progeny manifests as with abnormal sexual differentiation. We discovered significant correlation between pupal abnormalities feminization subsequent adults exemplified by relative abundance ovarian testicular tissues. All intersex individuals were they expressed determining factor, Nix . Further, our analysis sex-specific splicing doublesex fruitless transcripts demonstrated presence both female splice variants indicating that disrupted. A comparative transcriptomic revealed similar expression levels most female-associated genes reproductive organs carcasses intersexual normal females. Moreover, intersexes had largely gene testes but downregulation accessory glands when compared males. conclude evolving hybrid incompatibilities involve disruption accompanied changes associated

Language: Английский

Citations

0