DNA methylation correlates with transcriptional noise in response to elevated pCO2 in the eastern oyster (Crassostrea virginica) DOI Creative Commons
Yaamini R. Venkataraman, Ariana S. Huffmyer, Samuel J. White

et al.

Current Zoology, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 1, 2024

Abstract Ocean acidification significantly affects marine calcifiers like oysters, warranting the study of molecular mechanisms DNA methylation that contribute to adaptive plasticity in response environmental change. However, a consensus has not been reached on extent which modules gene expression, and turn plasticity, invertebrates. In this study, we investigated impact pCO2 expression eastern oyster, Crassostrea virginica. After 30-day exposure control (572 ppm) or elevated (2827 ppm), whole-genome bisulfite sequencing (WGBS) RNA-seq data were generated from adult female gonad tissue male sperm samples. Although differentially methylated loci (DMLs) identified females (89) males (2916), there no expressed genes only one transcript females. body impacted other forms activity sperm, such as maximum number transcripts per changes predominant expressed. Elevated increased variability (transcriptional noise) but decreased noise females, suggesting sex-specific role regulation. Functional annotation with transcript-level containing DMLs revealed several enriched biological processes potentially involved response, including apoptotic pathways signal transduction, well reproductive functions. Taken together, these results suggest may regulate maintain homeostasis conditions could play key resilience

Language: Английский

DNA methylation correlates with transcriptional noise in response to elevated pCO2 in the eastern oyster (Crassostrea virginica) DOI Creative Commons
Yaamini R. Venkataraman, Ariana S. Huffmyer, Samuel J. White

et al.

Current Zoology, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 1, 2024

Abstract Ocean acidification significantly affects marine calcifiers like oysters, warranting the study of molecular mechanisms DNA methylation that contribute to adaptive plasticity in response environmental change. However, a consensus has not been reached on extent which modules gene expression, and turn plasticity, invertebrates. In this study, we investigated impact pCO2 expression eastern oyster, Crassostrea virginica. After 30-day exposure control (572 ppm) or elevated (2827 ppm), whole-genome bisulfite sequencing (WGBS) RNA-seq data were generated from adult female gonad tissue male sperm samples. Although differentially methylated loci (DMLs) identified females (89) males (2916), there no expressed genes only one transcript females. body impacted other forms activity sperm, such as maximum number transcripts per changes predominant expressed. Elevated increased variability (transcriptional noise) but decreased noise females, suggesting sex-specific role regulation. Functional annotation with transcript-level containing DMLs revealed several enriched biological processes potentially involved response, including apoptotic pathways signal transduction, well reproductive functions. Taken together, these results suggest may regulate maintain homeostasis conditions could play key resilience

Language: Английский

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