cGAS-like receptors drive a systemic STING-dependent host response in Drosophila DOI Creative Commons

Xianlong Ai,

Huimin Deng, Xiaoyan Li

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(12), P. 115081 - 115081

Published: Dec. 1, 2024

cGAS-like receptor (cGLR)-stimulator of interferon genes (STING) recently emerged as an important pathway controlling viral infections in invertebrates. However, its exact contribution at the organismal level remains uncharacterized. Here, we use STING::GFP knockin reporter Drosophila flies to document activation vivo. Four tissues strongly respond injection cyclic dinucleotide 3'2'- guanosine monophosphate-adenosine monophosphate (cGAMP): central nervous system, midgut, Malpighian tubules, and genital ducts. The pattern induction injected with 3'2'-cGAMP or infected by two viruses different tropism suggests that is induced a systemic signal produced virus-infected cells. Accordingly, ectopic expression cGLR2 fat body induces STING signaling remote cGLR1/2-dependent activity transferred females during mating. Furthermore, infection can alter sleep cGLR1/2- STING-dependent manner. Altogether, our results reveal host response Drosophila.

Language: Английский

Evolutionary immunology to explore original antiviral strategies DOI Open Access
Jean‐Luc Imler, Hua Cai, Carine Meignin

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

Over the past 25 years, field of evolutionary developmental biology (evo–devo) has used genomics and genetics to gain insight on mechanisms underlying evolution morphological diversity animals. Evo–devo exploits key that conserved toolkits development (e.g. Hox genes) are in animals produce genetic novelties provide adaptation a new environment. Like development, immunity is forged by interactions with environment, namely microbial world. Yet, when it comes study immune defence invertebrates, interest primarily focuses evolutionarily molecules also present humans. Here, focusing antiviral immunity, we argue genes not humans represent an unexplored resource for discovery strategies. We review recent findings cGAS-STING pathway explain how cyclic dinucleotides produced cGAS-like receptors may be investigate portfolio broad range species. This will set stage evo–immuno approaches, exploiting investment defences made metazoans over hundreds millions years evolution. article part theme issue ‘Sculpting microbiome: host factors determine respond colonization’.

Language: Английский

Citations

11
Tissue specific innate immune responses impact viral infection in Drosophila DOI Creative Commons
Elisha Segrist, S. D. Miller,

Beth Gold

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(11), P. e1012672 - e1012672

Published: Nov. 4, 2024

All organisms sense and respond to pathogenic challenge. Tissue-specific responses are required combat pathogens infecting distinct cell types. Cyclic dinucleotides (CDNs) produced endogenously downstream of pathogen recognition or by themselves which bind STING activate NF-kB-dependent antimicrobial gene expression programs. It remains unknown whether there immune CDNs in Drosophila tissues. Here, we investigated tissue specific CDN-STING uncovered differences gene-induction patterns across tissues that play important roles viral infections. Using tissue-and cell-specific genetic studies found dSTING the fat body controls CDN-induced dSTING-regulated 1 ( Srg1 ) but not 2 Srg2 3 Srg3 ). In contrast, gastrointestinal tract largely . We is antiviral against natural fly C virus human arthropod-borne Rift Valley Fever (RVFV), other viruses including Sindbis dengue virus. Furthermore, has an role controlling RVFV infection ovary implications understanding vertical transmission mosquitoes. Overall, our study underscores importance tissue-specific immunity highlights complex regulation pathway.

Language: Английский

Citations

4
Regulation of detoxifying enzymes expression and restriction of picorna-like virus infection by natural polysaccharide extracts in Drosophila cells DOI

Gabrielle Haas,

Mélodie Seiler,

Jenny Nguyen

et al.

Virology, Journal Year: 2025, Volume and Issue: unknown, P. 110513 - 110513

Published: March 1, 2025

Language: Английский

Citations

0
Humoral immunity in insects: Antimicrobial peptides and other host defense peptides DOI
Mark A. Hanson,

Léna Hédelin

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
An evolutionary perspective to innate antiviral immunity in animals DOI Creative Commons
João T. Marques, Carine Meignin, Jean‐Luc Imler

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(9), P. 114678 - 114678

Published: Aug. 27, 2024

Viruses pose a significant threat to cellular organisms.Innate antiviral immunity encompasses both RNA-and protein-based mechanisms designed sense and respond infections, fundamental aspect present in all living organisms.A potent RNA-based mechanism is RNA interference, where small RNA-programmed nucleases target viral RNAs.Protein-based often rely on the induction of transcriptional responses triggered by recognition infections through innate immune receptors.These involve upregulation genes aimed at countering infections.In this review, we delve into recent advances understanding diversification animals.An evolutionary perspective gains losses diverse animals coupled mechanistic studies model organisms such as fruit fly Drosophila melanogaster essential provide deep that can be translated new strategies treatment diseases.

Language: Английский

Citations

3
cGAS-like receptors drive a systemic STING-dependent host response in Drosophila DOI Creative Commons

Xianlong Ai,

Huimin Deng, Xiaoyan Li

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(12), P. 115081 - 115081

Published: Dec. 1, 2024

cGAS-like receptor (cGLR)-stimulator of interferon genes (STING) recently emerged as an important pathway controlling viral infections in invertebrates. However, its exact contribution at the organismal level remains uncharacterized. Here, we use STING::GFP knockin reporter Drosophila flies to document activation vivo. Four tissues strongly respond injection cyclic dinucleotide 3'2'- guanosine monophosphate-adenosine monophosphate (cGAMP): central nervous system, midgut, Malpighian tubules, and genital ducts. The pattern induction injected with 3'2'-cGAMP or infected by two viruses different tropism suggests that is induced a systemic signal produced virus-infected cells. Accordingly, ectopic expression cGLR2 fat body induces STING signaling remote cGLR1/2-dependent activity transferred females during mating. Furthermore, infection can alter sleep cGLR1/2- STING-dependent manner. Altogether, our results reveal host response Drosophila.

Language: Английский

Citations

3