Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties DOI Open Access

Harihar M. Mohan,

Martín Tejedor Fernández,

Camellia Huang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

ABSTRACT The human genome is replete with sequences derived from foreign elements including endogenous retrovirus-like proteins of unknown function. Here we show that UBQLN2, a ubiquitin-proteasome shuttle factor implicated in neurodegenerative diseases, regulated by the linked actions two proteins, RTL8 and PEG10. confers on UBQLN2 ability to complex regulate PEG10, core component stress granules, drives recruitment granules under various conditions, but can only do so when present. Changes PEG10 levels further remodel kinetics granule disassembly overall composition incorporating select extracellular vesicle proteins. Within forms virus-like particles, underscoring structural heterogeneity this class biomolecular condensates. Together, these results reveal an unexpected link between pathways cellular proteostasis

Language: Английский

Positive Selection Drives the Evolution of the Structural Maintenance of Chromosomes (SMC) Complexes DOI Open Access
Diego Forni, Alessandra Mozzi, Manuela Sironi

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(9), P. 1159 - 1159

Published: Sept. 3, 2024

Structural Maintenance of Chromosomes (SMC) complexes are an evolutionary conserved protein family. In most eukaryotes, three SMC have been characterized, as follows: cohesin, condensin, and SMC5/6 complexes. These involved in a plethora functions, defects genes can lead to increased risk chromosomal abnormalities, infertility, cancer. To investigate the evolution complex mammals, we analyzed their selective patterns extended phylogeny. Signals positive selection were identified for condensin NCAPG, two (SMC5 NSMCE4A), all cohesin with almost exclusive meiotic expression (RAD21L1, REC8, SMC1B, STAG3). For latter, rates correlate during female meiosis, positively selected sites fall intrinsically disordered regions (IDRs). Our results support growing evidence that IDRs fast evolving, they likely contribute adaptation through modulation phase separation. We suggest natural signals may be result different pressures: host-pathogen arms race complexes, intragenomic conflict is similar described centromeres telomeres.

Language: Английский

Citations

1
Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties DOI Open Access

Harihar M. Mohan,

Martín Tejedor Fernández,

Camellia Huang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

ABSTRACT The human genome is replete with sequences derived from foreign elements including endogenous retrovirus-like proteins of unknown function. Here we show that UBQLN2, a ubiquitin-proteasome shuttle factor implicated in neurodegenerative diseases, regulated by the linked actions two proteins, RTL8 and PEG10. confers on UBQLN2 ability to complex regulate PEG10, core component stress granules, drives recruitment granules under various conditions, but can only do so when present. Changes PEG10 levels further remodel kinetics granule disassembly overall composition incorporating select extracellular vesicle proteins. Within forms virus-like particles, underscoring structural heterogeneity this class biomolecular condensates. Together, these results reveal an unexpected link between pathways cellular proteostasis

Language: Английский

Citations

1