A Robust and Orthogonal Far-Red Light Sensor for Gene Expression Control in Escherichia coli DOI
Yueyang Sun, Mengran Xu, Baiyang Wang

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Optogenetics has emerged as a powerful tool for regulating cellular processes due to its noninvasive nature and precise spatiotemporal control. Far-red light (FRL) increasingly been used in the optogenetic control of mammalian cells low toxicity high tissue penetration. However, robust orthogonal FRL sensors are lacking bacteria. Here, we established an sensor Escherichia coli with maximum dynamic range exceeding 230-fold based on RfpA-RfpC-RfpB (RfpABC) signaling system that regulates far-red photoacclimation (FaRLiP) cyanobacteria. We identified conserved DNA motif promoter sequences Chl f synthase gene other genes FaRLiP clusters, termed light-regulatory (FLR) motif, which enables light-responsive activation expression through interaction RfpB. Based FLR simplified FLR-containing promoters characterized their abilities ranges, can be utilized different synthetic biology scenarios. Additionally, one or two motifs present at loci within cluster, providing further FRL-inducible resources. The exhibits effective suppression under low-intensity white light, respectively, remains functional darkness. In conclusion, this study advances understanding regulatory mechanisms cyanobacteria provides applications.

Language: Английский

A Robust and Orthogonal Far-Red Light Sensor for Gene Expression Control in Escherichia coli DOI
Yueyang Sun, Mengran Xu, Baiyang Wang

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Optogenetics has emerged as a powerful tool for regulating cellular processes due to its noninvasive nature and precise spatiotemporal control. Far-red light (FRL) increasingly been used in the optogenetic control of mammalian cells low toxicity high tissue penetration. However, robust orthogonal FRL sensors are lacking bacteria. Here, we established an sensor Escherichia coli with maximum dynamic range exceeding 230-fold based on RfpA-RfpC-RfpB (RfpABC) signaling system that regulates far-red photoacclimation (FaRLiP) cyanobacteria. We identified conserved DNA motif promoter sequences Chl f synthase gene other genes FaRLiP clusters, termed light-regulatory (FLR) motif, which enables light-responsive activation expression through interaction RfpB. Based FLR simplified FLR-containing promoters characterized their abilities ranges, can be utilized different synthetic biology scenarios. Additionally, one or two motifs present at loci within cluster, providing further FRL-inducible resources. The exhibits effective suppression under low-intensity white light, respectively, remains functional darkness. In conclusion, this study advances understanding regulatory mechanisms cyanobacteria provides applications.

Language: Английский

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