Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 25, 2024
Language: Английский
Journal of Neurochemistry, Journal Year: 2025, Volume and Issue: 169(1)
Published: Jan. 1, 2025
Epitranscriptomic regulation of cell functions involves multiple post-transcriptional chemical modifications coding and non-coding RNA that are increasingly recognized in studying human brain disorders. Although rodent models presently widely used neuroepitranscriptomic research, the zebrafish (Danio rerio) has emerged as a useful promising alternative model species. Mounting evidence supports importance CNS function, providing additional insights into epitranscriptomic mechanisms underlying wide range Here, we discuss recent data on role regulation, with particular focus models, well evaluate current problems, challenges, future directions research this field molecular neurochemistry.
Language: Английский
Citations
0
BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)
Published: March 18, 2025
Oocyte maturation is crucial for female fertility and embryonic development, encompassing nuclear cytoplasmic maturation. Supportive cells of follicles, such as granulosa cells, are essential oocyte growth Oocytes can achieve without during in vitro (IVM). However, there still a higher chance incomplete these oocytes with mature nuclei compared cultured cells. have lower fertilization rates developmental potential than ones, although underlying mechanisms poorly understood. Identifying key genes signaling pathways associated help further elucidate the maturing process understand impact immature on throwing insights into strategy to improve success rate assisted reproductive technologies. Our study investigated murine IVM yielded vivo (IVO). Even though could they showed featuring levels reactive oxygen species, mitochondrial density, proportions abnormal distributions cortical granules. Of note, cytoplasm had distinct transcriptional profiles. In oocytes, we observed deficient mRNA restoration regulatory cellular division, potentially affecting development. Differentially expressed (DEGs) between were identified be highly different pre-implantation stages, MII oocyte, 8-cell stage, ICM stage. Identified DEGs enriched energy metabolic pathways. These observations indicated that impeded development might result gene expressions We show important both oocytes. Abnormal expression may defects
Language: Английский
Citations
0
Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 168934 - 168934
Published: Jan. 1, 2025
tRNAs are essential molecules in protein synthesis, responsible for translating the four-nucleotide genetic code into corresponding amino acid sequence. RNA modifications play a crucial role influencing tRNA folding, structure, and function. These modifications, ranging from simple methylations to complex hypermodified species, distributed throughout molecule. Depending on their type position, they contribute accuracy efficiency of decoding by participating network interactions. The enzymatic processes introducing these equally intricate diverse, adding further complexity. As result, studying faces limitations at multiple levels. This review addresses challenges involved manipulating function discusses experimental strategies possibilities overcome obstacles.
Language: Английский
Citations
0
Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 169091 - 169091
Published: March 1, 2025
Language: Английский
Citations
0
Biomolecules, Journal Year: 2024, Volume and Issue: 14(10), P. 1340 - 1340
Published: Oct. 21, 2024
As the most ancient RNA, transfer RNAs (tRNAs) play a more complex role than their constitutive function as amino acid transporters in protein synthesis process. The transcription and maturation of tRNA cells are subject to stringent regulation, resulting formation tissue- cell-specific pools with variations overall abundance, composition, modification, charging levels. heterogeneity contributes facilitating histocyte-specific expression patterns is involved diverse biological processes. Moreover, tRNAs can be recognized by various RNase under physiological pathological conditions generate tRNA-derived small (tsRNAs) serve regulatory Here, we summarize these recent insights into highlight advances regulation tsRNA biogenesis modifications. We synthesize mechanisms embryonic development, cell fate determination, epigenetic inheritance regulation. also discuss potential clinical applications based on new knowledge diagnostic prognostic biomarkers therapeutic strategies for multiple diseases.
Language: Английский
Citations
3
The EMBO Journal, Journal Year: 2024, Volume and Issue: 43(22), P. 5747 - 5779
Published: Oct. 14, 2024
Abstract Embryogenesis entails dramatic shifts in mRNA translation and turnover that reprogram gene expression during cellular proliferation differentiation. Codon identity modulates stability early vertebrate embryogenesis, but how the composition of tRNA pools is matched to translational demand unknown. By quantitative profiling repertoires zebrafish embryos maternal-to-zygotic transition, we show zygotic are established after onset gastrulation, succeeding major wave transcription. Maternal distinct, their reprogramming does not result a better match codon content transcriptome. Instead, find an increase global at gastrulation sensitizes decoding rates supply, thus destabilizing maternal mRNAs enriched slowly translated codons. Translational activation temporally coincide with TORC1 activity which phosphorylates inactivates RNA polymerase III repressor Maf1a/b. Our data indicate switch translation, rather than reprogramming, determines codon-dependent decay embryogenesis.
Language: Английский
Citations
1
Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 25, 2024
Language: Английский
Citations
1