Profiling local translatomes and RNA binding proteins of somatosensory neurons reveals specializations of individual axons

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

SUMMARY Individual neurons have one or more axons that often extend long distances and traverse multiple microenvironments. However, it is not known how the composition of individual established locally modulated to enable neuronal function plasticity. Here, we use spatial translatomics identify local axonal translatomes in anatomically functionally specialized dorsal root ganglia (DRG). DRG central peripheral opposite directions distinct microenvironments somatosensation. Using Translating Ribosome Affinity Purification RNA sequencing, generated a comprehensive resource mRNAs preferentially translated within each axon. Locally proteins include pain receptors, ion channels, translational machinery, which establish electrophysiologic properties regenerative capacities for We RNA-binding associated with sorting transporting related mRNAs. These findings provide resources addressing translation shapes organization enables subcellular neuroplasticity. HIGHLIGHTS Distinct are localized axons. Axonal govern capacity, electrophysiology. The RBP, SFPQ, coordinates mRNA towards somatosensory translatome data can be explored at painseq.shinyapps.io/CompartmentTRAP/.

Language: Английский

Compartmentalization of multiple metabolic enzymes and their preparation in vitro and in cellulo

Biochimica et Biophysica Acta (BBA) - General Subjects, Journal Year: 2025, Volume and Issue: unknown, P. 130787 - 130787

Published: March 1, 2025

Language: Английский