NAIL-MS Elucidates Crucial tRNA U34 Modifications in Response to Heat Stress across Eukaryotes and Prokaryotes DOI Creative Commons
Alexandre Magno Vicente,

Katarzyna Hencel,

Jannick Schicktanz

et al.

Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 169228 - 169228

Published: June 1, 2025

Global warming leads to rising temperatures, necessitating organismal adaptation at the cellular level. One potential mechanism for maintaining proteome integrity during stress is of tRNA modifications. While modification reprogramming has been well-studied under chemical stressors, its role in heat remains unclear. To address this, we performed a comparative analysis modifications Arabidopsis thaliana, Saccharomyces cerevisiae, Caenorhabditis elegans, Dictyostelium discoideum, and Escherichia coli stress. We assessed abundance 30 modified nucleosides using isotope dilution mass spectrometry control conditions. A. thaliana showed similar diversity compared other eukaryotes, suggesting conservation across species. Under stress, overall levels were largely stable, with no significant changes such as dihydrouridine N4-acetylcytidine. However, one four per organism altered, uridine position 34 (U34) being most prominent change. Here, pulse-chase NAIL-MS (nucleic acid labeling coupled spectrometry) experiments E. S. cerevisiae revealed that U34 occurred not only pre-existing tRNAs but also newly transcribed tRNAs. These results suggest existing adapt an early response while are reprogrammed ensure long-term survival prolonged heat. Our findings highlight adaptation.

Language: Английский

NAIL-MS Elucidates Crucial tRNA U34 Modifications in Response to Heat Stress across Eukaryotes and Prokaryotes DOI Creative Commons
Alexandre Magno Vicente,

Katarzyna Hencel,

Jannick Schicktanz

et al.

Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 169228 - 169228

Published: June 1, 2025

Global warming leads to rising temperatures, necessitating organismal adaptation at the cellular level. One potential mechanism for maintaining proteome integrity during stress is of tRNA modifications. While modification reprogramming has been well-studied under chemical stressors, its role in heat remains unclear. To address this, we performed a comparative analysis modifications Arabidopsis thaliana, Saccharomyces cerevisiae, Caenorhabditis elegans, Dictyostelium discoideum, and Escherichia coli stress. We assessed abundance 30 modified nucleosides using isotope dilution mass spectrometry control conditions. A. thaliana showed similar diversity compared other eukaryotes, suggesting conservation across species. Under stress, overall levels were largely stable, with no significant changes such as dihydrouridine N4-acetylcytidine. However, one four per organism altered, uridine position 34 (U34) being most prominent change. Here, pulse-chase NAIL-MS (nucleic acid labeling coupled spectrometry) experiments E. S. cerevisiae revealed that U34 occurred not only pre-existing tRNAs but also newly transcribed tRNAs. These results suggest existing adapt an early response while are reprogrammed ensure long-term survival prolonged heat. Our findings highlight adaptation.

Language: Английский

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