Dietary phytic acid damages the intestinal mucus barrier and structural integrity in the grass carp (Ctenopharyngodon idella)

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110300 - 110300

Published: March 1, 2025

Language: Английский

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Dietary Glyceryl Polyethylene Glycol Ricinoleate as an Additive to Improve Intestinal Health in Post-Weaning Piglets

Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 983 - 983

Published: March 29, 2025

Early weaning in intensive pig production induces stress, compromising gastrointestinal health. Poor fat digestion results from the piglets' underdeveloped digestive system. Dietary emulsifiers can enhance utilization, and glyceryl polyethylene glycol ricinoleate (GPGR) has been shown to improve performance. This study evaluated GPGR's effects on intestinal health weaned piglets a commercial farm. A total of 380 just (21 days old) were divided two groups 190 animals each (in four replicates) that received either basal diet (control) or + 350 g/ton GPGR pharmaceutical formulation as top dress. Blood samples collected at pre-established days, sampling occurred 15 post-weaning. Plasma cortisol, citrulline, morphology, mucus quality, enzymatic activity, volatile fatty acids (VFAs), cecal microbiota analyzed. did not alter plasma cortisol but increased citrullinemia (P: 0.024), suggesting greater enterocyte functional mass. showed improved morphology (greater villus height, height:crypt depth ratio, absorption area, p < 0.05) higher maltase activity (p ≤ 0.014). VFAs, bacterial adherence mucus, goblet cell counts unaffected. Firmicutes Actinobacteria 0.014 P: 0.045, respectively) while reducing Proteobacteria 0.001). In conclusion, dietary promotes by improving epithelial structure, function, balance, representing promising strategy support overcoming early nursery phase.

Language: Английский

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Adjusting the composition of gut microbiota prevents the development of post-stroke depression by regulating the gut-brain axis in mice

Journal of Affective Disorders, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Carvacrol Protects IPEC-J2 Cells from Oxidative Stress by Suppressing Autophagy

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3495 - 3495

Published: April 8, 2025

Oxidative stress impairs intestinal function and causes poor growth performance in piglets. Carvacrol is a natural essential oil, its anti-oxidative anti-inflammatory activities the intestines of piglets have been reported many studies. However, mechanisms underlying these protective effects against oxidative remain unclear. This study aimed to investigate possible pathway carvacrol porcine intestine under using an vitro model. Porcine epithelial cells (IPEC-J2) were treated with hydrogen peroxide (H2O2), inducer, stress. We found that ameliorated H2O2-induced loss cell viability, apoptosis, reduced intracellular reactive oxygen species (ROS) malondialdehyde (MDA) levels. mitochondrial ROS generation increased citrate synthase activity during Furthermore, attenuated increase autophagy marker LC3II-to-I ratio accumulation lysosomes autolysosomes induced by H2O2. The protein expression mitophagy PINK1, H2O2, was also treatment. Metformin-activated diminished on viability MDA levels H2O2 treatment, indicating inhibition necessary for carvacrol-induced protection IPEC-J2 In conclusion, this demonstrated mechanism exerted relieving excessive weaning

Language: Английский

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Hydroxy-Selenomethionine Supplementation During Gestation and Lactation Improve Reproduction of Sows by Enhancing the Antioxidant Capacity and Immunity Under Heat Stress Conditions

Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 525 - 525

Published: April 27, 2025

The objective of this study was to determine whether hydroxy-selenomethionine (OH-SeMet) exerts better protective effects on sows against heat stress than sodium selenite (SeNa) or seleno-yeast (SeY). A total 60 (Landrace × Yorkshire) were randomly allocated into the three groups and fed a base diet supplemented with SeNa, SeY, OH-SeMet at 0.3 mg Se/kg under condition for reproductive cycle. Compared SeNa could more effectively sustain offspring growth performance, as evidenced by an increased number live-born piglets, higher litter weight day 21, greater body gain from days 1 21. effective in supporting endogenous redox systems, shown enhanced levels TXNRD GSH reduced GSSG serum sows, improved T-AOC, TXNRD, alongside decreased MDA heightened T-AOC jejunum piglets. Furthermore, among two tested organic Se sources, SeY regulating immune responses compared SeNa. inflammation-related markers CRP, HP, MAP, LPS, IL-1β, IL-6, TNF-α, some all which their offspring. In addition, also showed glucose, TG, NEFA levels, along elevated insulin sows. Correspondingly, forms Se, particularly those gut protection sows’ offspring, indicated crypt depth villus height/crypt ratio duodenum, jejunum, ileum Specifically, upregulated expression selenoproteins (GPX6, TXNRD3, GPX4, SELENON), tight junction protein (ZO-1), host defense peptide gene (pBD1, pBD2, pBD3, NPG3, NPG4), downregulating inflammation factor (IL-1β, IL-6 TNF-α) pro-apoptotic (P53) Taken together, mitigated adverse induced

Language: Английский

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Sulforaphane regulates cell proliferation and induces apoptotic cell death mediated by ROS-cell cycle arrest in pancreatic cancer cells

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: Aug. 29, 2024

Background Pancreatic cancer (PC), sometimes referred to as pancreatic ductal adenocarcinoma (PDAC), is a major cause of global mortality from cancer. very aggressive and devastating kind cancer, characterized by limited options for therapy low possibilities survival. Sulforaphane (SFN), naturally occurring sulfur-containing compound, believed possess anti-inflammatory, anti-obesity, anti-cancer characteristics. Objective However, efficient preventative treatment measures are essential SFN has been studied its ability suppress cell proliferation induce apoptosis. Methods Here, induced cytotoxicity apoptosis in PDAC lines such MIA PaCa-2 PANC-1 cells, evaluated cytotoxicity, colony formation, western blot analysis, fluorescence-activated sorting (FACS), reactive oxygen species (ROS) detection, caspase-3 activity assay, immunofluorescence mitochondrial membrane potential assay. Results In inhibited survival dose-dependent manner. The activation caspase zymogens results cleaved PARP caspase-3, which associated with an accumulation the sub G1 phase. Furthermore, increased ROS level γH2A.X expression while decreasing (ΔΨm). Notably, scavenger N-Acetyl-L-cysteine (NAC) was shown reverse SFN-induced level. Subsequently, cycle arrest induction Trojan horse eliminate cells via ROS-mediated pathways were used inhibit cells. Conclusion Collectively, our data demonstrates that death follows pathway, making it viable target therapeutic interventions against

Language: Английский

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Enhanced gut damage and microbial imbalance in bullfrog tadpoles (Lithobates catesbeiana) exposed to polystyrene microplastics under high-temperature conditions

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126339 - 126339

Published: May 1, 2025

Language: Английский

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GPx1 deficiency confers increased susceptibility to ferroptosis in macrophages from individuals with active Crohn’s disease

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(12)

Published: Dec. 18, 2024

Abstract Intestinal cell death is a defining feature of Crohn’s disease (CD), major form inflammatory bowel disease. The focus on this aspect enteric inflammation has mainly been epithelial cells, while other types such as stromal and myeloid cells have received less attention. Hypothesising that decreased macrophage viability in an oxidative environment could be contributing factor to the pathophysiology CD, we found monocyte-derived macrophages from individuals with active CD (but not those clinical remission) increased sensitivity induced by H 2 O . Molecular biology pharmacological studies ruled out apoptosis necroptosis, lipid peroxidation surface expression transferrin receptor implicated ferroptosis mechanism -induced death: was supported suppression -cytotoxicity liproxstatin-1, inhibitor ferroptosis. Selenoproteins are important antioxidants, selenium deficiency can CD. Despite normal dietary intake selenium, intestinal had protein and/or mRNA selenoprotein, glutathione peroxidase (GPx)-1. Knockdown GPx1 healthy volunteers resulted reminiscent observed In summary, susceptibility death, may facilitated, at least part, reduced antioxidant GPx1. We suggest monocytes recruited gut renders these vulnerable reactive oxygen species-evoked unraveling participation pathway reveal novel therapeutic approaches chronic condition.

Language: Английский

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GPx1 deficiency confers increased susceptibility to ferroptosis in macrophages from individuals with active Crohn’s disease

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 5, 2024

Intestinal cell death is a defining feature of Crohn’s disease (CD), major form inflammatory bowel disease. The focus on this aspect enteric inflammation has mainly been epithelial cells, while other types such as stromal and myeloid cells have received less attention. Hypothesising that decreased macrophage viability in an oxidative environment could be contributing factor to the pathophysiology CD, we found monocyte-derived macrophages from individuals with active CD (but not those clinical remission) increased sensitivity induced by H2O2. Molecular biology pharmacological studies ruled out apoptosis necroptosis, lipid peroxidation surface expression transferrin receptor implicated ferroptosis mechanism H2O2-induced death: was supported suppression H2O2-cytotoxicity liproxstatin-1, inhibitor ferroptosis. Selenoproteins are important antioxidants, selenium deficiency can CD. Despite normal dietary intake selenium, intestinal had protein and/or mRNA selenoprotein, glutathione peroxidase (GPx)-1. Knockdown GPx1 healthy volunteers resulted reminiscent observed In summary, susceptibility death, may facilitated, at least part, reduced antioxidant GPx1. We suggest monocytes recruited gut renders these vulnerable reactive oxygen species-evoked unravelling participation pathway reveal novel therapeutic approaches chronic condition.

Language: Английский

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