Vaccines, Journal Year: 2024, Volume and Issue: 12(12), P. 1390 - 1390
Published: Dec. 11, 2024
The immune response to SARS-CoV-2 infection is highly complex, involving a dynamic interplay between the innate and adaptive systems [...].
Language: Английский
PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0309880 - e0309880
Published: Feb. 10, 2025
The continuous threats posed by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), the virus that causes COVID-19, including emergence of potentially more infectious and deadly variants, necessitate ongoing studies to uncover novel detailed mechanisms driving disease severity. Using single-cell transcriptomics, we conducted a secondary data analysis bronchoalveolar lavage fluid (BALF) from COVID-19 patients varying severities healthy controls comprehensively examine immune responses. We observed significant cell alterations correlating with In severe cases, macrophages showed upregulation pro-inflammatory genes TNFα IL1β, contributing inflammation tissue damage. Neutrophils exhibited increased activation, marked S100A8, CXCL8, IL1β expression, extended viability reduced phagocytosis. Genes such as MCL1 HIF1α supported viability, while MSR1 MRC1 indicated Enhanced formation neutrophil extracellular traps (NETs) clearance, NET-associated markers, were linked thrombo-inflammation organ Both neutrophils in cases impaired efferocytosis, decreased expression TREM2 downregulation FCGR3B neutrophils, leading accumulation apoptotic cells exacerbating inflammation. characterized M1 high milder had M2 elevated PPARγ. Dendritic (DCs) proportions attenuated MHC class I (HLA-A, HLA-B, HLA-C) co-stimulatory molecules (CD80, CD86), alongside cytochrome c indicating antigen presentation enhanced apoptosis. NK T demonstrated altered receptor gene activation markers IFNγ ISG15, suggesting paradoxical state exhaustion. This highlights critical role dysregulated neutrophil, macrophage, dendritic cell, NK, responses identifying potential therapeutic targets providing insights into disease.
Language: Английский
Citations
0
Frontiers in Pediatrics, Journal Year: 2025, Volume and Issue: 13
Published: Feb. 24, 2025
Recent studies have underscored the importance of genetic factors in predicting COVID-19 susceptibility and severity. While cytokine storms are crucial disease severity, predisposition significantly influences immune responses. Our study examined genes related to SARS-CoV-2 invasion (ACE2 rs2074192) interferon-induced immunity (IFNAR2 rs2236757, TYK2 rs2304256, OAS1 rs10774671, OAS3 rs10735079). Additionally, we investigated linked Kawasaki (CD40 rs4813003, FCGR2A rs1801274, CASP3 rs113420705) that play roles immunogenesis. The pilot study, which involved 75 pediatric patients aged one month 17 years [43 with active COVID-19, children multisystem inflammatory syndrome (MIS-C), 15 healthy controls], was conducted Ternopil, Ukraine. Gene polymorphism studied for all patients. ELISA kits were used interleukin studies, including Human IL-1β (Interleukin 1 Beta), IL-6 6), IL-8 8), IL-12 12), IFN-α (Interferon Alpha), TNF-α (Tumor Necrosis Factor Alpha). Statistical analysis performed using IBM SPSS Statistics 21 GraphPad Prism 8.4.3. identified significant gene-cytokine associations ACE2 rs2074192 T allele correlated increased IL-1β, IL-6, IL-8, TNF-α. IFNAR2 rs2236757 A elevated levels low levels, while rs10774671 carriers also exhibited lower levels. prognostically determining infected SARS-CoV-2. gene rs10735079 associated changes precisely a high level. CD40 rs4813003 C had higher IL-12. results our revealed correlation between rs1801274 (A/G). rs113420705 led an increase IL-6. These findings enhance understanding may hold promise developing targeted interventions providing personalized medical approach each patient.
Language: Английский
Citations
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Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 25, 2025
Hypertension is one of the most common risk factors for COVID-19 clinical progression. The identification plasma biomarkers anticipating worse outcomes and to better understand shared mechanisms between hypertension are needed. A hypothesis-generating study was designed compare proteomics metabolomics 22 hypertensives (HT) 41 non-hypertensives (nHT) patients with unfavorable total 43 molecules were significantly differed HT (n = 22) nHT 41). Random Forest (RF) analysis identified myo-inositol, gelsolin phosphatidylcholine (PC) 32:1 as top distinguishing nHT. Plasma myo-inositol higher (P 0.03 P 0.02, respectively) PC lower 0.03) in compared Biological processes like stress response blood coagulation, along KEGG pathways including ascorbate aldarate metabolism 0.021) linoleic acid 0.028), altered hypertensive There a clear link severe COVID-19. Key biological consider improving prognosis quality life who become infected SARS-CoV-2 include oxidative stress, metabolism, lipid immune system inflammation.
Language: Английский
Citations
0
Vaccines, Journal Year: 2024, Volume and Issue: 12(12), P. 1390 - 1390
Published: Dec. 11, 2024
The immune response to SARS-CoV-2 infection is highly complex, involving a dynamic interplay between the innate and adaptive systems [...].
Language: Английский
Citations
0