Function of Rice High-Affinity Potassium Transporters in Pollen Development and Fertility DOI
Weihong Li, Mengqi Li, Shen Li

et al.

Plant and Cell Physiology, Journal Year: 2022, Volume and Issue: 63(7), P. 967 - 980

Published: May 4, 2022

Abstract Plant High-affinity K+ transporters/K+ uptake permeases/K+ transporters (HAK/KUP/KT) have been predicted as membrane H+-K+ symporters in facilitating and distribution, while their role seed production remains to be elucidated. In this study, we report that OsHAK26 is preferentially expressed anthers husks located the Golgi apparatus. Knockout of either or plasma symporter gene OsHAK1 OsHAK5 both Nipponbare Dongjin cultivars caused distorted anthers, reduced number germination rate pollen grains. Seed-setting assay by reciprocal cross-pollination between mutants oshak26, oshak1, oshak5 wild types confirmed each HAK transporter foremost for viability, seed-setting grain yield. Intriguingly, pollens oshak26 showed much thinner wall were more vulnerable desiccation than those oshak1 oshak5. vitro revealed was dramatically affected external concentration. The results suggest maintaining development fertility may relate its proper cargo sorting construction walls, likely relates transcellular transport activity.

Language: Английский

Clathrin-mediated endocytosis facilitates the internalization ofMagnaporthe oryzaeeffectors into rice cells DOI Creative Commons
Ely Oliveira‐Garcia, Tej Man Tamang, Jungeun Park

et al.

The Plant Cell, Journal Year: 2023, Volume and Issue: 35(7), P. 2527 - 2551

Published: March 28, 2023

Abstract Fungi and oomycetes deliver effectors into living plant cells to suppress defenses control processes needed for infection. Little is known about the mechanism by which these pathogens translocate effector proteins across plasma membrane cytoplasm. The blast fungus Magnaporthe oryzae secretes cytoplasmic a specialized biotrophic interfacial complex (BIC) before translocation. Here, we show that within BICs are packaged punctate membranous compartments occasionally observed in host Live cell imaging with fluorescently labeled rice (Oryza sativa) showed puncta colocalize CLATHRIN LIGHT CHAIN 1, component of clathrin-mediated endocytosis (CME). Inhibiting CME using virus-induced gene silencing chemical treatments resulted swollen lacking puncta. By contrast, fluorescent marker colocalization, silencing, inhibitor studies failed support major role clathrin-independent Effector localization patterns indicated translocation occurs underneath appressoria invasive hyphal growth. Taken together, this study provides evidence mediated suggests M. coopting endocytosis.

Language: Английский

Citations

53
Genome-wide identification of the adaptor protein complexes and its expression patterns analysis in foxtail millet (Setaria italica L.) DOI Creative Commons
Dan Wang, Min Su,

Jian‐Hong Hao

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 2, 2025

Adapter proteins (APs) complex is a class of heterotetrameric complexes comprising 4-subunits with important regulatory functions in eukaryotic cell membrane vesicle trafficking. Foxtail millet (Setaria italica L.) significant C4 model plant for monocotyledon studies, and trafficking may plays crucial role various life activities related to growth development. Despite this importance, studies on AP foxtail have been lacking. This research conducted genome-wide identification systematical analysis millet. 33 SiAP genes were identified classified into 7 groups, distributed unevenly across 9 chromosomes Among these genes, 11 segmental duplication pairs found. Out the 24 exhibited collinear relationships Setaria viridis, while only one showed relationship Arabidopsis thaliana. Gene structure motif composition investigated understand function evolution genes. Furthermore, promoter region contains 49 cis-elements that are associated responses light, hormones, abiotic stress, The interaction network between was analyzed, there strong interactions among proteins. Expression patterns different organs developmental stages investigated. majority expressed multiple tissues, some being predominantly specific tissues. Subsequently, we selected SiAP4M SiAP2M validation subcellular localization. signal 35 S:: SiAP4M: GFP (Long) (Short) fused primarily observed nucleus, SiAP2M: widely vesicles. Overall, study presents comprehensive map These findings not administer understanding biological development but also offer insights enhancing genetic breeding crop.

Language: Английский

Citations

1
Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole DOI Creative Commons
Jierui Zhao,

Mai Thu Bui,

Juncai Ma

et al.

The Journal of Cell Biology, Journal Year: 2022, Volume and Issue: 221(12)

Published: Oct. 19, 2022

Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, maturation, i.e., delivery and fusion with vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, directly interacts marker ATG8 localizes on both membranes of autophagosome. form normally Arabidopsis thaliana cfs1 mutants, but their is disrupted. CFS1’s function evolutionarily conserved plants, as it also autophagosomes plays a role autophagic flux liverwort Marchantia polymorpha. CFS1 regulates by bridging multivesicular body-localized ESCRT-I component VPS23A, leading formation amphisomes. Similar CFS1-ATG8 interaction, disrupting CFS1-VPS23A interaction blocks renders plants sensitive nitrogen starvation. Altogether, our results reveal vacuolar sorting hub

Language: Английский

Citations

30
K63-linked ubiquitin chains are a global signal for endocytosis and contribute to selective autophagy in plants DOI Creative Commons
Bushra Saeed, Florian Deligne, Carla Brillada

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(7), P. 1337 - 1345.e5

Published: March 1, 2023

Language: Английский

Citations

21
Nitric oxide alleviates programmed cell death induced by cadmium in Solanum lycopersicum seedlings through protein S-nitrosylation DOI

Dengjing Huang,

Xinfang Chen,

Fahong Yun

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 931, P. 172812 - 172812

Published: May 3, 2024

Language: Английский

Citations

7
Structural and Evolutionary Aspects of Plant Endocytosis DOI
Michael Kraus, Roman Pleskot, Daniël Van Damme

et al.

Annual Review of Plant Biology, Journal Year: 2024, Volume and Issue: 75(1), P. 521 - 550

Published: Jan. 18, 2024

Endocytosis is an essential eukaryotic process that maintains the homeostasis of plasma membrane proteome by vesicle-mediated internalization. Its predominant mode operation utilizes polymerization scaffold protein clathrin forming a coat around vesicle; therefore, it termed clathrin-mediated endocytosis (CME). Throughout evolution, machinery mediates CME marked losses, multiplications, and innovations. employs limited number conserved structural domains folds, whose assembly connections are species dependent. In plants, many grouped into ancient multimeric complex, TPLATE which occupies central position as interaction hub for endocytic machinery. this review, we provide overview current knowledge regarding aspects plant CME, draw comparisons to other model systems. To do so, have taken advantage recent developments with respect artificial intelligence-based structure prediction.

Language: Английский

Citations

6
Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator DOI
Peng Wang, Wei Siao, Xiuyang Zhao

et al.

Nature Plants, Journal Year: 2023, Volume and Issue: 9(2), P. 355 - 371

Published: Jan. 12, 2023

Language: Английский

Citations

14
Terpenoid Transport in Plants: How Far from the Final Picture? DOI Creative Commons
Olivia Costantina Demurtas, Alessandro Nicolia, Gianfranco Diretto

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(3), P. 634 - 634

Published: Feb. 1, 2023

Contrary to the biosynthetic pathways of many terpenoids, which are well characterized and elucidated, their transport inside subcellular compartments secretion reaction intermediates final products at short- (cell-to-cell), medium- (tissue-to-tissue), long-distance (organ-to-organ) levels still poorly understood, with some limited exceptions. In this review, we aim describe state art several terpene classes that have important physiological ecological roles or represent high-value bioactive molecules. Among tens thousands terpenoids identified in plant kingdom, only less than 20 been from point view localization. Most secreted apoplast stored vacuoles by action ATP-binding cassette (ABC) transporters. However, little information is available regarding movement terpenoid plastids endoplasmic reticulum cytosol. Through a description mechanisms cytosol- plastid-synthesized terpenes, attempt provide hypotheses, suggestions, general schemes about trafficking different substrates, intermediates, products, might help develop novel strategies approaches allow for future identification transporters uncharacterized.

Language: Английский

Citations

14
Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology DOI Creative Commons
Liang Zhang,

Xinlin Liang,

Tomáš Takáč

et al.

Plant Biotechnology Journal, Journal Year: 2022, Volume and Issue: 21(2), P. 250 - 269

Published: Oct. 7, 2022

Summary In plants, membrane compartmentalization requires vesicle trafficking for communication among distinct organelles. Membrane proteins involved in are highly dynamic and can respond rapidly to changes the environment cellular signals. Capturing their localization dynamics is thus essential understanding mechanisms underlying vesicular pathways. Quantitative mass spectrometry imaging approaches allow a system‐wide dissection of proteome, characterization ligand‐receptor pairs determination secretory, endocytic, recycling vacuolar this review, we highlight major proteomics methods employed determine location, distribution abundance within given routes. We focus particular on methodologies elucidation protein interactions connections downstream signalling outputs. Finally, assess biological applications exploring different subcellular processes.

Language: Английский

Citations

22
A century journey of organelles research in the plant endomembrane system DOI

Xiaohong Zhuang,

Ruixi Li, Liwen Jiang

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(5), P. 1312 - 1333

Published: Jan. 16, 2024

Abstract We are entering an exciting century in the study of plant organelles endomembrane system. Over past century, especially within 50 years, tremendous advancements have been made complex cell to generate a much clearer and informative picture organelles, including molecular/morphological features, dynamic/spatial behavior, physiological functions. Importantly, all these discoveries achievements identification characterization system would not possible without: (1) innovations timely applications various state-of-art biology tools technologies for organelle research; (2) continuous efforts developing characterizing new markers by community; (3) landmark studies on elusive organelles. While molecular aspects results individual extensively reviewed, development techniques research is less appreciated. As one ASPB Centennial Reviews “organelle biology,” here we aim take journey across plants highlighting important (or technologies) key scientists that contributed visualize then highlight leading systems.

Language: Английский

Citations

4