Nature Plants, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 16, 2025
Language: Английский
Science, Journal Year: 2022, Volume and Issue: 377(6605)
Published: July 7, 2022
Plant nucleotide-binding leucine-rich repeat-containing (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain sense pathogen effectors to enable TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) activity for immune signaling. TIR-NLR signaling requires the helper NLRs N requirement gene 1 (NRG1), Activated Disease Resistance (ADR1), and Enhanced Susceptibility (EDS1), which forms a heterodimer each of its paralogs Phytoalexin Deficient 4 (PAD4) Senescence-Associated Gene 101 (SAG101). Here, we show that TIR-containing proteins catalyze production 2'-(5''-phosphoribosyl)-5'-adenosine monophosphate (pRib-AMP) diphosphate (pRib-ADP) in vitro planta. Biochemical structural data demonstrate EDS1-PAD4 is complex pRib-AMP pRib-ADP, allosterically promote interaction ADR1-L1 but not NRG1A. Our study identifies TIR-catalyzed pRib-ADP as missing link TIR through likely second messengers plant immunity.
Language: Английский
Citations
163
Science, Journal Year: 2022, Volume and Issue: 377(6605)
Published: July 7, 2022
Plant pathogen-activated immune signaling by nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain converges on Enhanced Disease Susceptibility 1 (EDS1) and its direct partners, Phytoalexin Deficient 4 (PAD4) or Senescence-Associated Gene 101 (SAG101). TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) produces molecules to promote exclusive EDS1-PAD4 EDS1-SAG101 interactions helper NLR subclasses. In this work, we show that TIR-containing proteins catalyze adenosine diphosphate (ADP)-ribosylation of triphosphate (ATP) ADP ribose (ADPR) through ADPR polymerase-like NADase activity, forming ADP-ribosylated ATP (ADPr-ATP) ADPr-ADPR (di-ADPR), respectively. Specific binding ADPr-ATP di-ADPR allosterically promotes interaction N requirement gene 1A (NRG1A) in vitro planta. Our data reveal enzymatic activity TIRs enables specific activation the EDS1-SAG101-NRG1 immunity branch.
Language: Английский
Citations
160
International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(12), P. 6758 - 6758
Published: June 17, 2022
Plant pathogens are one of the main factors hindering breeding cash crops. Pathogens, including oomycetes, fungus, and bacteria, secrete effectors as invasion weapons to successfully invade propagate in host plants. Here, we review recent advances made field plant-pathogen interaction models action mechanisms phytopathogenic effectors. The illustrates how from different species use similar distinct strategies infect We classify interactions according infestation process: targeting physical barriers for disruption, creating conditions conducive infestation, protecting or masking themselves, interfering with cell physiological activity, manipulating plant downstream immune responses. investigation functioning pathogen contributes improved understanding molecular interactions. This has important theoretical value is practical significance pathology disease resistance genetics breeding.
Language: Английский
Citations
124
Essays in Biochemistry, Journal Year: 2022, Volume and Issue: 66(5), P. 647 - 656
Published: June 14, 2022
Abstract The phytohormones salicylic acid (SA) and jasmonic (JA) are major players in plant immunity. Numerous studies have provided evidence that SA- JA-mediated signaling interact with each other (SA-JA crosstalk) to orchestrate immune responses against pathogens. At the same time, SA-JA crosstalk is often exploited by pathogens promote their virulence. In this review, we summarize our current knowledge of molecular mechanisms for modulations during pathogen infection.
Language: Английский
Citations
112
Cell, Journal Year: 2024, Volume and Issue: 187(9), P. 2095 - 2116
Published: April 1, 2024
Plant diseases cause famines, drive human migration, and present challenges to agricultural sustainability as pathogen ranges shift under climate change. breeders discovered Mendelian genetic loci conferring disease resistance specific isolates over 100 years ago. Subsequent breeding for underpins modern agriculture and, along with the emergence focus on model plants genetics genomics research, has provided rich resources molecular biological exploration last 50 years. These studies led identification of extracellular intracellular receptors that convert recognition microbe-encoded patterns or pathogen-delivered virulence effectors into defense activation. receptor systems, downstream responses, define plant immune systems have evolved since migration land ∼500 million Our current understanding provides platform development rational enhancement control many continue plague crop production.
Language: Английский
Citations
109
Science, Journal Year: 2022, Volume and Issue: 377(6605)
Published: July 7, 2022
In the 20th century, researchers studying animal and plant signaling pathways discovered a protein domain that is shared across diverse innate immune systems: Toll/interleukin-1/resistance gene (TIR) domain. The TIR found in several architectures was defined as an adaptor mediates protein-protein interactions immunity developmental pathways. However, studies of nerve degeneration animals-and subsequent breakthroughs plant, bacterial, archaeal systems-revealed domains possess enzymatic activities. We provide synthesis functions role various related products evolutionarily systems. These may ultimately guide interventions would span tree life, from treating human neurodegenerative disorders bacterial infections to preventing diseases.
Language: Английский
Citations
104
Nature Plants, Journal Year: 2022, Volume and Issue: 8(10), P. 1146 - 1152
Published: Oct. 14, 2022
Abstract Recent reports suggest that cell-surface and intracellular immune receptors function synergistically to activate robust defence against pathogens, but whether they co-evolve is unclear. Here we determined the numbers of in 350 species. Surprisingly, number receptor genes are predicted encode strongly correlated. We this consistent with mutual potentiation immunity initiated by being reflected concerted co-evolution size their repertoires across plant
Language: Английский
Citations
77
Plant Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21(12), P. 2433 - 2457
Published: Aug. 25, 2023
Plant-specific NAC proteins constitute a major transcription factor family that is well-known for its roles in plant growth, development, and responses to abiotic biotic stresses. In recent years, there has been significant progress understanding the functions of proteins. have highly conserved DNA-binding domain; however, their are diverse. Previous structure factors can be used as basis functional diversity. consist target-binding domain at N-terminus versatile C-terminal interacts with other A growing body research on helps us comprehend intricate signalling network transcriptional reprogramming facilitated by NAC-mediated complexes. However, most studies limited single function. Here, we discuss upstream regulators, regulatory components targets context prospective improvement strategies via biotechnology intervention, highlighting importance plants need further research.
Language: Английский
Citations
68
The EMBO Journal, Journal Year: 2023, Volume and Issue: 42(5)
Published: Jan. 2, 2023
Abstract Plant pathogens compromise crop yields. Plants have evolved robust innate immunity that depends in part on intracellular Nucleotide‐binding, Leucine rich‐Repeat (NLR) immune receptors activate defense responses upon detection of pathogen‐derived effectors. Most “sensor” NLRs detect effectors require the activity “helper” NLRs, but how helper support sensor NLR function is poorly understood. Many Solanaceae NRC (NLR‐Required for Cell death) class NLRs. We show here Rpi‐amr3, a from Solanum americanum , detects AVRamr3 potato late blight pathogen, Phytophthora infestans and activates oligomerization NRC2 NRC4 into high‐molecular‐weight resistosomes. In contrast, recognition P. effector AVRamr1 by another Rpi‐amr1 induces formation only resistosome. The activated oligomer becomes enriched membrane fractions. ATP‐binding motifs both Rpi‐amr3 are required resistosome formation, not interaction with its cognate effector. can be homologs other species. Mechanistic understanding will underpin engineering crops durable disease resistance.
Language: Английский
Citations
64
EMBO Reports, Journal Year: 2023, Volume and Issue: 24(10)
Published: Aug. 21, 2023
Plants coordinately use cell-surface and intracellular immune receptors to perceive pathogens mount an response. Intracellular events of pathogen recognition are largely mediated by the nucleotide binding leucine rich-repeat (NLR) classes. Upon perception, NLRs trigger a potent broad-spectrum reaction, usually accompanied form programmed cell death termed hypersensitive Some plant act as multifunctional singleton which combine detection signaling. However, can also function in higher order pairs networks functionally specialized interconnected receptors. In this article, we cover basic aspects NLR biology with emphasis on networks. We highlight some recent advances structure, function, activation discuss emerging topics such modulator NLRs, suppression bioengineering. Multi-disciplinary approaches required disentangle how these receptor evolve. Answering questions holds potential deepen our understanding system unlock new era disease resistance breeding.
Language: Английский
Citations
62