Physiological and Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 102722 - 102722
Published: April 1, 2025
Language: Английский
Horticulture Research, Journal Year: 2025, Volume and Issue: 12(6)
Published: Feb. 18, 2025
Abstract Flesh fruits are essential for human health, but pathogen infection poses a threat to fruit production and postharvest storage. The hormone salicylic acid (SA) its metabolites, such as sugar conjugates methyl salicylate (MeSA), play crucial role in regulating plant immune responses. However, the UDP-glycosyltransferases (UGTs) responsible modulating SA metabolism have yet be identified, further investigation is needed elucidate involvement response. Here, we identified PpUGT74F2 an enzyme with highest transcription level peach fruit, catalyzing biosynthesis of glucoside (SAG), not MeSAG formation fruit. Furthermore, Monilinia fructicola, which causes brown rot disease, led reduced expression PpUGT74F2, resulting significant decrease SAG content increase MeSA levels. Transgenic tomatoes expressing heterologous increased susceptibility gray mold. Interestingly, overexpressing did affect levels dramatically response infection, accompanied by significantly pathogen-related (PR) genes transgenic tomatoes. This study highlights that acts negative regulatory factor immunity through distinct mechanism previously reported plants.
Language: Английский
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0
Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(2), P. 164 - 164
Published: Feb. 19, 2025
The biotrophic fungus Ustilago maydis, which causes smut disease in maize, secretes numerous proteins upon plant colonization. Some of them, termed effectors, help to evade defenses and manipulate cellular processes within the host. function many specifically secreted during infection remains elusive. In this study, we biochemically characterized one such protein, UMAG_00027, that is highly expressed infection. We show UMAG_00027 a protein with lectin-like fold therefore term it Llp1 (lectin-like-protein 1). decorated fungal cell wall cells grown axenic culture or proliferating planta, agreement its potential sugar-binding ability. were unable identify precise sugar moieties are bound by Llp1. CRISPR/Cas9-mediated deletion llp1 reveals gene not essential for virulence. A structural search shows presence several other U. maydis might compensate ∆llp1 mutants. speculate part family redundant functions.
Language: Английский
Citations
0
Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 491, P. 137923 - 137923
Published: March 13, 2025
Language: Английский
Citations
0
Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown
Published: March 13, 2025
The infiltration of Nicotiana benthamiana with Agrobacterium tumefaciens (agroinfiltration) has become a routine expression platform for plant science and molecular pharming, yet this remains to be further optimized. We recently showed that N. silenced the cold shock protein (CSP) receptor (CORE) enables 8-fold more GFP production in older, 6–8-week-old plants, which are normally not used because low transient efficiencies (Dodds et al., 2023). Here, we investigated whether can also increase levels routinely younger, 5-week-old juvenile by silencing immunity-related genes. selected 21 genes encoding proteins act at different immune system (Table S1). Besides CORE, receptor-encoding WAK1 (Wall-associated Protein Kinase), CERK1 (Chitin Elicitor Receptor Kinase-1), BAK1 (BRI1-associated SOBIR1 (Suppressor BIR1-1) RE02 (Receptor SCPs). tested signaling components such as F-box ACIF1 (Avr/Cf-induced F-box-1); lipase-like EDS1 (Enhanced Disease Susceptibility-1) SAG101 (Senescence-associated Gene-101); CDPK (Calcium-dependent MPK3/6 (MAP kinases-3 -6), Nod-like helper receptors NRC2/3/4 (NLR Required Cf Signaling) chaperone CRT3a (Calreticulin-3a). included required stress hormone signaling, including PAL (Phenylalanine Ammonia Lyase), ICS (Isochorismate Synthase), NPR1 (Nonexpressor PR genes-1), EIN2 (Ethylene-insensitive-2) WRKY transcription factors. Finally, AHA2 (Arabidopsis H+-ATPase 2) RBOHB (Respiratory Burst Oxidase Homolog B). Genes phytoene desaturase (PDS) ß-glucuronidase (GUS) were positive negative controls silencing, respectively. resynthesized fragments published previously S1) novel targeting ACIF1, CDPK, 2023, Tables S2 S3). Tobacco Rattle Virus (TRV) vectors, each carrying fragment these immunity agroinfiltrated into 2-week-old seedlings plants three weeks later. Transcript targeted downregulated (Figure At stage, no strong phenotypes observed TRV-inoculated except photobleaching TRV::PDS dwarfed TRV::BAK1 TRV::CDPK small, chlorotic TRV::ICS S2). Leaf discs TRV::RBOHB reduced oxidative burst upon flg22 treatment S3), consistent effective Silenced 1:1:1 mixture Agrobacteria delivering 'traffic light' reporters: bioluminescent AgroLux bacteria (Jutras 2021, orange), mixed cassettes cytonuclear (cGFP, green) secreted RFP (sRFP, red). fluorescence leaf taken 5 days post-infiltration (5 dpi) was significantly higher TRV::ICS, TRV::CERK1, TRV::NPR1 TRV::EIN2 when compared TRV::GUS 1a). same control S4a). No altered detected TRV::CORE CORE (Wang 2016). bioluminescence similar between S4b), indicating does population levels. Scanning leaves subsequent experiments confirmed or increased GFP-fluorescence >4-fold even >10-fold, GUS 1b,c). Also, significant, 1.7-fold TRV::EIN2, S5), initial screen. effects imply CERK1, encode important barriers 1d). is receptor-like kinase involved perception chitin (Miya 2007) peptidoglycan (Willmann 2011). transcriptional regulator responses induced salicylic acid (SA) (Spoel Dong, 2024). chloroplastic enzyme producing isochorismate, precursor SA unaltered TRV::PAL indicates restricting produced via phenylalanine, but through isochorismate effect twice might depletion would only reduce signalling, negatively regulate vir gene (Yuan 2007). To enhance without relying on VIGS, disrupting open reading frames using genome editing stacking mutant So far, deletion two S6), indeed caused accumulation 1e) enhanced IgG S7). These genome-edited lines hold potential boosting benthamiana. thank Urszula Pyzio excellent care, Sarah Rodgers, Caroline O'Brien Patricia Bowman technical support. This project financially supported Interdisciplinary DTC DDT00060 (ID) DDT00230 (EW); BBSRC projects BB/R017913/1 (PB) BB/S003193/1 (MS); ERC 101019324 (RH). RALH conceived project; ILD ECW performed most help MS PB SS; YT, MHAJJ TB provided VIGS constructs JS npr11/npr1b mutant; ILD, wrote manuscript all authors. None declared. data supports findings study available supplementary material article. Appendix S1 Materials methods. Figure Novel TRV silence target Phenotypes VIGSed plants. S3 Suppressed flg22-induced S4 Impact sRFP luminescence. S5 increases S6 npr1 double S7 Increased npr1a/npr1b Table VIGS. Used plasmids. silencing. oligonucleotides. Please note: publisher responsible content functionality any supporting information supplied Any queries (other than missing content) should directed corresponding author
Language: Английский
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0
Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)
Published: March 1, 2025
Pathogenesis-related (PR) proteins are critical defense signaling molecules induced by phytopathogens. They play a vital role in plant's pathways and innate immunity, particularly systemic acquired resistance (SAR) serve as key molecular markers of plant defense. Overexpressing PR genes, such chitinase, thaumatin, glucanase, thionin defensin, either individually or combination, have significantly boosted plants' responses against various pathogens. However, regulating the expression these versatile remain only partially understood. Plant hormones like salicylic acid (SA) jasmonic (JA) known for their well-established roles gene to pathogens other stress conditions. genes interact with components hormonal pathways, including receptors (e.g., NPR1 SA signaling), transcription factors MYC2 JA cis-regulating elements W-box), modulate responses. Recent studies highlighted contributions different immunity interactions process crosstalk, which helps coordinate activation. This review provides comprehensive overview proteins, complexity, crosstalk aiming understand improved pathogen resistance.
Language: Английский
Citations
0
Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109839 - 109839
Published: March 1, 2025
Language: Английский
Citations
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International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142462 - 142462
Published: March 1, 2025
Language: Английский
Citations
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Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 226, P. 113538 - 113538
Published: March 31, 2025
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
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Science Advances, Journal Year: 2025, Volume and Issue: 11(16)
Published: April 18, 2025
Isoprene, emitted by some plants, deters insect herbivory. However, the associated biochemical and physiological responses that confer herbivory resistance remain unknown. We used engineered isoprene-emitting (IE) non-emitting (NE) control tobacco plants to interpret isoprene-mediated defense against in plants. Hornworm larvae raised on IE exhibited stunted growth compared those NE Worms preferred feed rather than leaves, indicating deterrent effects of isoprene feeding. Worm feeding induced a greater increase jasmonic acid (JA), crucial hormone for resistance, leaves leaves. Assimilation rates were stably maintained suggesting protective role preserving photosynthetic efficiency during Wound-induced emission correlated with elevation key metabolites biosynthesis pathway. Our results highlight JA-priming functions provide insights into
Language: Английский
Citations
0