Tools for pathogen genetic surveillance: Lessons from the ash dieback invasion of Europe DOI Creative Commons
Jessica A. Peers, Richard M. Leggett, Matthew D. Clark

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(5), P. e1012182 - e1012182

Published: May 23, 2024

Human movement and climate change are exposing new environments to pathogens increasing the rate of invasions [1].Presently, most studied independently, requiring species-specific expertise, particularly at sampling stage.Genomic surveillance strategies can be applied identify species presence/absence but also within-species diversity, which may not currently capitalised upon.Here, we use ash dieback invasion Europe [2] frame a discussion on significance different types genomic variation biology review application developing microbiome sequencing technologies pathogen surveillance. What is dieback?European (Fraxinus excelsior) prevalent throughout Europe; for example, it third common tree in United Kingdom.Ash keystone with approximately 1,000 associated over 100 obligately/highly [3].The (see [4]), caused by Hymenoscyphus fraxineus, was first identified Estonia, 1992, has since spread west, reaching UK 2012 [2].In its native range East Asia, H. fraxineus saprophyte little effect host, F. mandshurica [5].In Europe, causes crown death least 2 [2].Ash estimated cost economy £7.6 billion 2029 [6].Slow disease symptoms [7], environmental conditions [8], varying resistance [9], propagule pressure [10] make estimating mortality rates difficult.Best estimates suggest 70% 85% [7].Models plant defence against focus genes evolved recognise gene products trigger immunity [11].Reduced susceptibility European appears mediated many meaning that both natural selection breeding programmes have lot work do bring all those together [12].H. within trade infected samplings wind consequently, found 50 km ahead infection front [13].Management diseases these scales difficult, so our attention turns introduction reintroduction.Surveillance measures could important an early or prevent subsequent genetic diversity focussing effort limiting exchanges between reservoirs critically adaptation.

Language: Английский

Tandem kinase proteins across the plant kingdom DOI
Tamara Reveguk, Andrii Fatiukha,

Evgenii Potapenko

et al.

Nature Genetics, Journal Year: 2025, Volume and Issue: 57(1), P. 254 - 262

Published: Jan. 1, 2025

Language: Английский

Citations

4
A wheat tandem kinase activates an NLR to trigger immunity DOI

Renjie Chen,

Jian Chen, Oliver R. Powell

et al.

Science, Journal Year: 2025, Volume and Issue: 387(6741), P. 1402 - 1408

Published: March 27, 2025

The role of nucleotide-binding leucine-rich repeat (NLR) receptors in plant immunity is well studied, but the function a class tandem kinases (TKs) that confer disease resistance wheat and barley remains unclear. In this study, we show SR62 locus digenic module encoding Sr62 TK an NLR (Sr62 ), identify corresponding AvrSr62 effector. binds to N-terminal kinase 1 , triggering displacement 2, which activates . Modeling mutation analysis indicated mediated by overlapping binding sites (i) on for 2 (ii) Understanding two-component complex may help engineering breeding plants durable resistance.

Language: Английский

Citations

3
Recent advances in nano-enabled immunomodulation for enhancing plant resilience against phytopathogens DOI Creative Commons

Hafiza Ayesha Masood,

Yetong Qi,

Muhammad Khubaib Zahid

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 7, 2024

Plant diseases caused by microbial pathogens pose a severe threat to global food security. Although genetic modifications can improve plant resistance; however, environmentally sustainable strategies are needed manage diseases. Nano-enabled immunomodulation involves using engineered nanomaterials (ENMs) modulate the innate immune system of plants and enhance their resilience against pathogens. This emerging approach provides unique opportunities through ability ENMs act as nanocarriers for delivering immunomodulatory agents, nanoprobes monitoring immunity, nanoparticles (NPs) that directly interact with cells trigger responses. Recent studies revealed application nanoscale agrochemicals strengthen immunity biotic stress enhancing systemic resistance pathways, modulating antioxidant defense systems, activating defense-related pathways reshaping plant-associated microbiomes. However, key challenges remain in unraveling complex mechanisms which influence molecular networks, assessing long-term environmental impacts, developing biodegradable formulations, optimizing targeted delivery methods. review comprehensive investigation latest research on nano-enabled strategies, potential action, highlights future perspectives overcome existing disease management.

Language: Английский

Citations

6
Ripening and rot: How ripening processes influence disease susceptibility in fleshy fruits DOI Creative Commons
Shan Li, Yu Zhao, Pan Wu

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(9), P. 1831 - 1863

Published: July 17, 2024

Fleshy fruits become more susceptible to pathogen infection when they ripen; for example, changes in cell wall properties related softening make it easier pathogens infect fruits. The need high-quality fruit has driven extensive research on improving resistance important crops such as tomato (Solanum lycopersicum). In this review, we summarize current progress understanding how during ripening affect by pathogens. These physical barriers that limit entry, the epidermis and its cuticle, along with other defenses growth, preformed induced defense compounds. plant immune system also protects recognizing initiating responses involving reactive oxygen species production, mitogen-activated protein kinase signaling cascades, jasmonic acid, salicylic ethylene, abscisic acid signaling. phytohormones regulate an intricate web of transcription factors (TFs) activate mechanisms, including expression pathogenesis-related genes. tomato, regulators, RIPENING INHIBITOR NON_RIPENING, not only but influence against Moreover, members ETHYLENE RESPONSE FACTOR (ERF) family play pivotal distinct roles defense, different being regulated phytohormones. We discuss interaction ripening-related defense-related TFs Mediator complex. As processes climacteric non-climacteric share many similarities, these have broad applications across fruiting crops. Further individual contributions ERFs will inform efforts diminish disease susceptibility ripe fruit, satisfy growing demand decrease food waste economic losses.

Language: Английский

Citations

3
From Lesions to Lessons: Two Decades of Filamentous Plant Pathogen Genomics DOI Creative Commons
Wagner C. Fagundes,

Yu-Seng Huang,

Susanne Häußler

et al.

Molecular Plant-Microbe Interactions, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Filamentous plant pathogens pose a severe threat to food security. Current estimates suggest up 23% yield losses pre- and post-harvest diseases these are projected increase due climate change (Singh et al. 2023; Chaloner 2021; Stukenbrock Gurr Fisher 2012; Steinberg 2020). Understanding how filamentous emerge, spread adapt their hosts new environmental niches is crucial address devastating impact on global agriculture. Over the past two decades, genomics has emerged as central technology advance our understanding of population dynamics coevolution pathogens. The first genome pathogen, blast fungus Magnaporthe oryzae, was sequenced 20 years ago. Since then, pathogen not only provided deep genomic makeup populations, but fundamental for gene discovery, disease diagnostics, accelerated molecular biology. In merely we have witnessed several revolutions in field, including reference genomes many pathogens, scale thousands individuals, long-read chromosome conformation sequencing highly continuous assemblies recent intersections structural Here, commemorate 20th anniversary by presenting important technological advances (Fig. 1) lessons learned from decades genomics, with focus cereal diseases.

Language: Английский

Citations

0
Comprehensive review of Plasmodiophora brassicae: pathogenesis, pathotype diversity, and integrated control methods DOI Creative Commons
Xueliang Xu, Caiyun Wu, Fan Zhang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 6, 2025

Clubroot disease is an important of cruciferous crops worldwide caused by Plasmodiophora brassicae. The pathogen P. brassicae can infect almost all crops, resulting in a reduction yield and quality the host plant. first part this review outlines process infestation, effectors, physiological pathotypes identification systems. latter highlights summarizes various current control measures research progress on clubroot. Finally, we propose strategic concept for sustainable management In conclusion, paper will help to deepen knowledge understanding integrated clubroot, lay solid foundation

Language: Английский

Citations

0
Unravelling mechanisms underlying phosphate-induced susceptibility to bakanae disease in rice DOI Creative Commons
Héctor Martín‐Cardoso,

Gerrit Bücker,

Iratxe Busturia

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: 15, P. 100766 - 100766

Published: Feb. 7, 2025

Language: Английский

Citations

0
Small Interfering RNAs as Critical Regulators of Plant Life Process: New Perspectives on Regulating the Transcriptomic Machinery DOI Open Access
Marta Puchta, Paulina Bolc, Aleksandra Pietrusińska

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(4), P. 1624 - 1624

Published: Feb. 14, 2025

Small interfering RNAs (siRNAs) are a distinct class of regulatory in plants and animals. Gene silencing by small is one the fundamental mechanisms for regulating gene expression. siRNAs critical regulators during developmental processes. have similar structures functions to but derived from double-stranded RNA may be involved directing DNA methylation target sequences. less well-studied than miRNA group, researchers continue identify new classes that appear at specific stages particular tissues, revealing more complex mode siRNA action previously thought. This review characterizes their biogenesis process focuses on presenting known regulation plant development responses biotic abiotic stresses. The also highlights exciting potential future research this field, proposing methods detecting bioinformatic pathway identifying functions.

Language: Английский

Citations

0
Stomatal‐based immunity differentiation across vascular plant lineages DOI Open Access

Yuan‐Yuan Zeng,

Xu‐Dong Liu,

Guang‐Qian Yao

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 15, 2025

Summary Some plants are known to actively close their stomata in the presence of foliar pathogens, inhibiting pathogen entry into leaves, leading ‘stoma‐based immunity’ as first line defense. However, variation stoma‐based innate immunity across diversity vascular remains unclear. Here, we investigated stomatal response and guard cell signaling pathway various seed plant, fern, lycophyte species when exposed bacterial pathogens or pathogen‐associated molecular patterns (PAMPs). We observed active closure 10 bacteria PAMPs, whereas none nine fern one exhibited this response. The PAMP flg22‐induced reactive oxygen burst was all species, but downstream events, including cytosolic Ca 2+ accumulation, nitric oxide production, ion fluxes, vacuolar acidification, cytoplasmic pH elevation, compartmentation, disaggregation actin cytoskeleton cells, were only plants. No such changes representatives ferns lycophytes. Our findings suggest a major difference regulation between lycophytes under study's conditions, unveiling physiological biophysical mechanisms that may have underpinned evolutionary adaptation responses attacks

Language: Английский

Citations

0
Utilizing Plant Synthetic Biology to Accelerate Plant-Microbe Interactions Research DOI Creative Commons
Xiaohan Yang, Joanna Tannous, Tomás A. Rush

et al.

BioDesign Research, Journal Year: 2025, Volume and Issue: unknown, P. 100007 - 100007

Published: March 1, 2025

Language: Английский

Citations

0