Conservation of molecular responses upon viral infection in the non-vascular plantMarchantia polymorpha DOI Creative Commons

Eric Ros-Moner,

Tamara Jiménez-Góngora,

Luis Villar-Martín

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 6, 2023

After plants transitioned from water to land around 450 million years ago, they faced novel pathogenic microbes. Their colonization of diverse habitats was driven by anatomical innovations like roots, stomata, and vascular tissue, which became central plant-microbe interactions. However, the impact these on plant immunity pathogen infection strategies remains poorly understood. Here, we explore plant-virus interactions in bryophyte Marchantia polymorpha gain insights into evolution relationships. Virome analysis reveals that is predominantly associated with RNA viruses. Comparative studies tobacco mosaic virus (TMV) show shares core defense responses but also exhibits unique features, such as a sustained wound response preventing viral spread. Additionally, general are equivalent those restricted tissues Nicotiana, suggesting evolutionary acquisition developmental results re-routing plants.

Language: Английский

Assembly and functional mechanisms of plant NLR resistosomes DOI
Shijia Huang, Ertong Li, Fangshuai Jia

et al.

Current Opinion in Structural Biology, Journal Year: 2025, Volume and Issue: 90, P. 102977 - 102977

Published: Jan. 13, 2025

Language: Английский

Citations

1
NRC Immune receptor networks show diversified hierarchical genetic architecture across plant lineages DOI
Foong‐Jing Goh, Ching‐Yi Huang, Lida Derevnina

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3399 - 3418

Published: June 26, 2024

Plants' complex immune systems include nucleotide-binding domain and leucine-rich repeat-containing (NLR) proteins, which help recognize invading pathogens. In solanaceous plants, the NRC (NLR required for cell death) family includes helper NLRs that form a genetic network with multiple sensor to provide resistance against However, evolution function of networks outside plants are currently unclear. Here, we conducted phylogenomic macroevolutionary analyses comparing identified from different asterid lineages found expanded significantly in most lamiids but not Ericales campanulids. Using transient expression assays Nicotiana benthamiana, showed simple campanulids, have high complexity lamiids. Phylogenetic grouped into three NRC0 subclades conserved, several family-specific show signatures diversifying selection. Functional revealed members partially interchangeable, whereas lack interchangeability. Our findings highlight distinctive evolutionary patterns asterids potential insights transferring disease across plant lineages.

Language: Английский

Citations

8
Evolutionary and immune‐activating character analyses of NLR genes in algae suggest the ancient origin of plant intracellular immune receptors DOI
Xingyu Feng, Qian Li, Yang Liu

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(5), P. 2316 - 2330

Published: July 7, 2024

SUMMARY Nucleotide‐binding leucine‐rich repeat (NLR) proteins are crucial intracellular immune receptors in plants, responsible for detecting invading pathogens and initiating defense responses. While previous studies on the evolution function of NLR genes were mainly limited to land evolutionary trajectory immune‐activating character algae remain less explored. In this study, genome‐wide gene analysis was conducted 44 chlorophyte species across seven classes charophyte five classes. A few but variable number genes, ranging from one 20, identified chlorophytes three charophytes, whereas no remaining algal genomes. Compared with genomes possess fewer or usually implying that expansion plants can be attributed their adaptation more complex terrestrial pathogen environments. Through phylogenetic analysis, domain composition conserved motifs profiling NBS domain, we detected shared lineage‐specific features between supporting common origin continuous green plant genes. Immune‐activation assays revealed both TNL RNL elicit hypersensitive responses Nicotiana benthamiana , indicating molecular basis activation has emerged early stage different types proteins. summary, results study suggest may have taken a role as ancestor plants.

Language: Английский

Citations

4
Conservation of molecular responses upon viral infection in the non-vascular plant Marchantia polymorpha DOI Creative Commons

Eric Ros-Moner,

Tamara Jiménez-Góngora,

Luis Villar-Martín

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 27, 2024

Language: Английский

Citations

3
A necrotizing toxin enables Pseudomonas syringae infection across evolutionarily divergent plants DOI Creative Commons

Kristina Grenz,

Khong‐Sam Chia, Emma K Turley

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

3
TaANK-TPR1 enhances wheat resistance against stripe rust via controlling gene expression and protein activity of NLR protein TaRPP13L1 DOI
Shuangyuan Guo, Feng Zhang, Xiaona Du

et al.

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Conserved effectors underpin the virulence of liverwort-isolated Pseudomonas in divergent plants DOI Creative Commons
K. E. P. Robinson,

Lucia Buric,

Kristina Grenz

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Plant pathogenic Pseudomonas species naturally antagonize a diverse range of flowering plants. While emerging research demonstrates that isolates belonging to the P. syringae complex colonize hosts, extent which these bacteria infect non-flowering plants like model liverwort Marchantia polymorpha remains unclear. Here, we identify natural associations between viridiflava and polymorpha. isolated from diseased liverworts in wild successfully re-infected M. under pure culture conditions, producing high planta bacterial densities causing prominent tissue maceration. Comparative genomic analysis Marchantia-associated identified core virulence machinery type III secretion system (T3SS) conserved effectors (AvrE HopM1) were essential for infection. Disease assays performed Nicotiana benthamiana further confirmed liverwort-associated an effector-dependent manner. Our work highlights as effective broad host pathogen relies on factors manipulate evolutionarily divergent

Language: Английский

Citations

0
Diversification of the “EDVID” packing motif underpins structural and functional variation in plant NLR coiled-coil domains DOI Creative Commons

Oliver Sulkowski,

Anna Ovodova,

Andrea Leisse

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: June 3, 2025

Summary Nucleotide-binding leucine-rich repeat receptors (NLRs) are critical in plant immunity and display remarkable allelic diversity. Coiled-coiled NLRs (CC-NLRs) the most widespread group of these found across flowering non-flowering plants. Here we investigate sequence conservation functional variation conserved EDVID motif α3-helix cell death inducing CC domain NLRs. We analyse our findings context published protein structures structure prediction. find that can serve as a predictor canonical CC-NLR function oligomeric assembly. also is accompanied by preceding acidic residues certain CC-NLRs with homology to Arabidopsis RPP8. The appearance this so-called preEDVID phylogeny plants its contribution underpins structural diversity motif. further show exist have lost suggesting subgroup, previously referred G10 -NLRs, functions different manner from mechanism. located helper NLR NRG1.1 linked activity.

Language: Английский

Citations

0
Phylogenomic insights into the diversity and evolution of RPW8‐NLRs and their partners in plants DOI

Huiyu Qin,

Junyuan Cheng,

Guan‐Zhu Han

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 120(3), P. 1032 - 1046

Published: Sept. 23, 2024

SUMMARY Plants use nucleotide‐binding leucine‐rich repeat receptors (NLRs) to sense pathogen effectors, initiating effector‐triggered immunity (ETI). NLRs containing RESISTANCE TO POWDERY MILDEW 8 domain (RNLs) function as “helper” in flowering plants and support the immune responses mediated by “sensor” cooperation with lipase‐EP fused proteins (EP proteins). Despite their crucial roles ETI, much remains unclear about evolutionary trajectories of RNLs functional partners EP proteins. Here, we perform phylogenomic analyses 90 plants, covering major diversity identify presence land green algae, expanding distribution RNLs. We uncover a neglected RNL group gymnosperms, besides canonical NRG1s ADR1s, observe drastic increase repertoire size conifers. Phylogenetic indicate that originated multiple times through shuffling, evolution underwent birth‐and‐death process. Moreover, trace origin back last common ancestor vascular plants. find both evolve mainly under negative selection, revealing strong constraints on function. Concerted losses positive correlation copy number are observed between sublineages, suggesting Together, our findings provide insights into plant helper NLRs, implications for predicting novel innate signaling modules.

Language: Английский

Citations

1
From algae to apples: The structural and functional conservation of NLRs DOI Creative Commons
Michael Busche, Bradley Laflamme

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(7), P. 2453 - 2454

Published: April 29, 2024

Plant species have been warding off microbial infections throughout their evolutionary history, which has led to the development of innate immune systems tailored sensing and responding pathogens.In plants, intracellular response is spearheaded by Nucleotide-Binding Leucine-Rich-Repeat Receptors, or NLRs, often detect "effector" virulence proteins used pathogens evade subvert host defenses.Upon activation, NLRs induce a suite defense responses, including accumulation hormones, induction immune-related genes, activation programmed cell death (Adachi et al. 2019).A key function oligomerization driven nucleotide-binding domain upon binding ATP.Such oligomers are referred as "resistosomes", but resistosomes differs between subclasses determined N-terminal domains subcellular localization.The oldest group in terms evolution contain an coiled-coil (CC) domain, some shown form calcium channels oligomerization.(Hu Chai 2023).While field NLR biology plants received enormous amount attention last five years, this focused almost entirely on angiosperms (or flowering plants).In issue, Khong-Sam Chia colleagues (Chia 2024) expand our understanding non-flowering plant lineages, identifying immunity-related mechanisms that likely place for hundreds millions years.

Language: Английский

Citations

0