The conserved ATPase PCH-2 controls the number and distribution of crossovers by antagonizing crossover formation in C. elegans

eLife, Journal Year: 2024, Volume and Issue: 13

Published: Oct. 30, 2024

Meiotic crossover recombination is essential for both accurate chromosome segregation and the generation of new haplotypes natural selection to act upon. This requirement known as assurance one example control. While conserved role ATPase, PCH-2, during meiotic prophase has been enigmatic, a universal phenotype when pch-2 or its orthologs are mutated change in number distribution crossovers. Here, we show that PCH-2 controls crossovers by antagonizing their formation. antagonism produces different effects at stages prophase: early prophase, prevents double-strand breaks from becoming crossover-eligible intermediates, limiting formation sites initial break homolog interactions. Later winnows contributing designation ultimately, assurance. We also demonstrate accomplishes this regulation through HORMAD, HIM-3. Our data strongly support model which PCH-2’s remodel HORMADs throughout destabilize precursors coordinate with synapsis, ensuring progressive implementation explaining function pachytene checkpoint

Language: Английский

Genomic divergence shaped the genetic regulation of meiotic homologous recombination inBrassicaallopolyploids

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Summary The tight regulation of meiotic recombination between homologs is disrupted in Brassica AAC allotriploids, a genomic configuration that may have facilitated the formation rapeseed ( napus L.) ∼7,500 years ago. Indeed, presence haploid C genome induces supernumerary crossovers homologous A chromosomes with dramatically reshaped distribution. However, genetic mechanisms driving this phenomenon and their divergence nascent established lineages remain unclear. To address these concerns, we generated hybrids carrying additional derived either from an lineage allotetraploid B. or its diploid progenitor oleracea . We then assessed variation across twelve populations by mapping male using Single Nucleotide Polymorphism markers evenly distributed sequenced genome. Our findings reveal C09 chromosome responsible for near pericentromeric regions. Interestingly, counterpart shows no significant effect on own, despite having similar content genes. showed influences crossover through inter-chromosomal epistatic interactions other specific chromosomes. These results provide new insights into emphasize role since allopolyploid

Language: Английский