The Optimized HaloTag Technology Provides a Versatile Tool for Cell Imaging in Plants DOI

Hongping Qian,

Changwen Xu,

Xinxiu Zuo

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The innovative HaloTag is a labeling technology that plays crucial role in advanced fluorescence imaging. However, due to the complexity of plant materials, application field plants still its infancy. To expand cells, we constructed six eukaryotic gene expression vectors with different localizations carrying labels, then transformed Arabidopsis thaliana and Nicotiana benthamiana, finally stained living cells using ligands. Live-cell imaging showed did not affect localization pattern biological functions target protein, by improving experimental conditions, optimized could be more effectively applied plants. Remarkably, first displayed exhibits superior performance compared conventional fluorescent proteins, including photostability, monomer properties, multicolor spatio-temporal differentiated labeling. More importantly, combination single-particle tracking reveals specific dynamics membrane proteins changes rearrangement ER-PM connectivity, providing powerful tool for analyzing protein dynamics. Taken together, this promising enriches toolkit studying spatiotemporal various processes.

Language: Английский

Necessity for modeling hormonal crosstalk in arabidopsis root development? DOI Creative Commons

Simon Moore,

Junli Liu, Chunli Chen

et al.

Trends in Plant Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

HighlightsPhytohormones play vital roles in root development. The activity of each hormone cannot change independently others. development mathematical models is required to understand their interplay.The necessity for modeling hormonal crosstalk Arabidopsis thaliana explored, and we discuss new insights that can be developed by artificial intelligence (AI). Important questions addressed include: regulation auxin biosynthesis cytokinin ethylene the simultaneous patterning auxin, cytokinin, ethylene? How does a given PIN-FORMED (PIN) other pin mutants, what role it ethylene-related gene expression related crosstalk?We propose one integrative model should integrate all experimental data elucidating growth.AbstractHormones plant Mathematical have been employed study functions. However, different research groups focus on aspects hormones therefore used growth as an system involves functions hormones. To use development, nature requires further interplay context diverse data. This opinion article discusses beyond We growth.

Language: Английский

Citations

0
Three-dimensional econstruction and autophagy process analysis reveal development pattern of articulated laticifers in Taraxacum kok-saghyz Rodin DOI Creative Commons
Meng Wang, Jie Qiu, Yifeng Zhou

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 228, P. 120857 - 120857

Published: March 19, 2025

Language: Английский

Citations

0
Unlocking the Potential of the RUBY Reporter System: How to Address Its Challenges in Plant‐Environment Interaction Research? DOI
Zijian Hu,

Shiya Shen,

Xi Zhang

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Summary statement We summarized limitations of RUBY and proposed using a regulatable promoter system to control expression, thereby alleviating the metabolic stress. aim inspire scientists optimize this reporter system, applying it study plant‐environment interactions in future.

Language: Английский

Citations

0
The Optimized HaloTag Technology Provides a Versatile Tool for Cell Imaging in Plants DOI

Hongping Qian,

Changwen Xu,

Xinxiu Zuo

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

The innovative HaloTag is a labeling technology that plays crucial role in advanced fluorescence imaging. However, due to the complexity of plant materials, application field plants still its infancy. To expand cells, we constructed six eukaryotic gene expression vectors with different localizations carrying labels, then transformed Arabidopsis thaliana and Nicotiana benthamiana, finally stained living cells using ligands. Live-cell imaging showed did not affect localization pattern biological functions target protein, by improving experimental conditions, optimized could be more effectively applied plants. Remarkably, first displayed exhibits superior performance compared conventional fluorescent proteins, including photostability, monomer properties, multicolor spatio-temporal differentiated labeling. More importantly, combination single-particle tracking reveals specific dynamics membrane proteins changes rearrangement ER-PM connectivity, providing powerful tool for analyzing protein dynamics. Taken together, this promising enriches toolkit studying spatiotemporal various processes.

Language: Английский

Citations

0