Graft incompatibility between pepper and tomato elicits an immune response and triggers localized cell death

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(12)

Published: Sept. 11, 2024

Language: Английский

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Plant disease dynamics in a changing climate: impacts, molecular mechanisms, and climate-informed strategies for sustainable management

Discover Agriculture, Journal Year: 2024, Volume and Issue: 2(1)

Published: Dec. 27, 2024

Climate change is increasingly conceded as a significant menace to global agricultural productivity, with plant diseases emerging critical challenge in this context. Plant can result an annual reduction of approximately US$220 billion impacting economies and socio-economic stability. To address these challenges, it crucial explore the intricate relationship between climatic variables occurrence, intensity, geographic distribution diseases, highlighting how shifts temperature, CO2 levels, precipitation patterns exacerbate pathogen virulence alter disease dynamics. This review examines historical projected impacts climate on vulnerable crops regions, emphasizing heightened risk food security. The molecular basis climate-modulated defense mechanisms dissected, offering insights into underlying biological processes that govern interactions. In response escalating threat, we discuss climate-informed management strategies, including early warning systems, adaptive surveillance, development resilient through biotechnological advancements such genetic engineering, gene silencing, genome editing. Additionally, role resource-saving technologies precision agriculture mitigating adverse repercussions health. concludes exploration research needs future perspectives, underscoring urgent need for interdisciplinary approaches safeguard sustainable productivity rapidly changing climate.

Language: Английский

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GhCNGC13 and 32 Act as Critical Links between Growth and Immunity in Cotton

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 1 - 1

Published: Dec. 19, 2023

Cyclic nucleotide-gated ion channels (CNGCs) remain poorly studied in crop plants, most of which are polyploid. In allotetraploid Upland cotton (Gossypium hirsutum), silencing GhCNGC13 and 32 impaired plant growth shoot apical meristem (SAM) development, while triggering autoimmunity. Both hormones (indole-3-acetic acid gibberellin) stress (abscisic acid, salicylic jasmonate) increased, leaf photosynthesis decreased. The silenced plants exhibited an enhanced resistance to Botrytis cinerea; however, Verticillium wilt was weakened, associated with LIPOXYGENASE2 (LOX2) downregulation. Transcriptomic analysis revealed 4835 differentially expressed genes (DEGs) functional enrichment immunity photosynthesis. These DEGs included a set transcription factors significant over-representation the HSF, NAC, WRKY families. Moreover, numerous members GhCNGC family were identified among DEGs, may indicate coordinated action. Collectively, our results suggested that functionally link photosynthesis, growth, immunity. We proposed play critical role “growth–defense tradeoff” widely observed crops.

Language: Английский

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Deficiencies in cluster‐2 ALA lipid flippases result in salicylic acid‐dependent growth reductions

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(2)

Published: Feb. 27, 2024

Abstract P4 ATPases (i.e . , lipid flippases) are eukaryotic enzymes that transport lipids across membrane bilayers. In plants, named Aminophospholipid (ALAs) and organized into five phylogenetic clusters. Here we generated an Arabidopsis mutant lacking all cluster‐2 ALAs ( ala8/9/10/11/12 ), which is the most highly expressed ALA subgroup in vegetative tissues. Plants harboring quintuple knockout (KO) show rosettes 2.2‐fold smaller display chlorotic lesions. A similar but less severe phenotype was observed ala10/11 double KO. The growth lesion phenotypes of mutants were reversed by expressing a NahG transgene, encodes enzyme degrades salicylic acid (SA). role for SA promoting further supported quantitative PCR assays showing increased mRNA abundance SA‐biosynthesis gene ISOCHORISMATE SYNTHASE 1 ICS1 ) two SA‐responsive genes PATHOGENESIS‐RELATED GENE PR1 PR2. Lesion also growing plants liquid media containing either low calcium (~0.1 mM) or high nitrogen concentrations (~24 mM), conditions known to suppress SA‐dependent autoimmunity. Yeast‐based fluorescent uptake revealed ALA10 ALA11 overlapping substrate specificities, including LysoPC signaling lipids. Together, these results establish biochemical functions ALA8–12 at least partially overlapping, deficiencies result autoimmunity has not been flippase with other

Language: Английский

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Graft incompatibility between pepper and tomato can be attributed to genetic incompatibility between diverged immune systems

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 1, 2024

Summary Graft compatibility is the capacity of two plants to form cohesive vascular connections. Tomato and pepper are incompatible graft partners; however, underlying cause rejection between these species remains unknown. We diagnosed incompatibility tomato diverse varieties based on weakened biophysical stability, decreased growth, persistent cell death using trypan blue TUNEL assays. Transcriptomic analysis in junction was performed RNA-sequencing, molecular signatures for response were characterized meta-transcriptomic comparisons with other biotic processes. show that broadly cultivars. These partners activate prolonged transcriptional changes highly enriched defense Amongst processes broad NLR upregulation hypersensitive response. Using transcriptomic datasets a variety stress treatments, we identified significant overlap genetic profile grafting plant parasitism. In addition, found over 1000 genes uniquely upregulated grafts. Based overactivity, DNA damage, have determined likely caused by incompatibility, which triggers hyperimmune-response.

Language: Английский

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