Current advances in the biosynthesis and sustainable production strategies of carotenoids and their multifaceted applications in the food industry: A comprehensive review

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105864 - 105864

Published: Jan. 1, 2025

Language: Английский

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Reassessing the contribution of TOMATO AGAMOUS-LIKE1 to fruit ripening by CRISPR/Cas9 mutagenesis

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(2)

Published: Jan. 22, 2024

Language: Английский

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Comprehensive metabolome and transcriptome analyses demonstrate divergent anthocyanin and carotenoid accumulation in fruits of wild and cultivated loquats

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 13, 2023

Eriobotrya is an evergreen fruit tree native to South-West China and adjacent countries. There are more than 26 loquat species known in this genus, while E. japonica the only yet domesticated produce fresh fruits from late spring early summer. Fruits of cultivated usually orange colored, contrast red color wild henryi (EH). However, mechanisms pigment formation during evolution be elucidated. To understand these, targeted carotenoid anthocyanin metabolomics as well transcriptomics analyses were carried out study. The results showed that β-carotene, violaxanthin palmitate rubixanthin laurate, totally accounted for over 60% colored carotenoids, major carotenoids peel ‘Jiefangzhong’ (JFZ) fruits. Total content JFZ about 10 times EH, expression levels PSY , ZDS ZEP 10.69 23.26 folds EH at ripen stage. Cyanidin-3- O -galactoside pelargonidin-3- predominant anthocyanins enriched peel. On contrary, both them almost undetectable JFZ, transcript F3H F3’H ANS CHS CHI 4.39 73.12 higher pigmentation. In summary, abundant deposition correlated with strong accumulation metabolites tightly associated notably upregulated expressions . This study was first demonstrate metabolic background how pigmentations evolved species, provided gene targets further breeding colorful via manipulation biosynthesis.

Language: Английский

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Silicon application improves tomato yield and nutritional quality

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 25, 2025

Silicon (Si) is a beneficial nutrient well-known for its functions in enhancing plant resistance to abiotic and biotic stresses. How Si application affects tomato yield quality underlying physiological mechanisms remain largely unclear. Our pot experiment showed that (45 kg ha⁻¹ Na₂SiO₃) significantly promoted accumulation of nitrogen, phosphorus, potassium, the shoot soil-cultured greenhouse. Such improved mineral nutrition favored Si-applied performance terms height, stem diameter, single fruit weight, yield, as indicated by significant increases 11.34%, 53.57%, 62.12%, 33.81%, respectively, when compared control (0 Na₂SiO₃). Higher catalase superoxide dismutase activities contrast lower concentrations hydrogen peroxide malondialdehyde suggested facilitated health. Importantly, upregulated expression phytoene synthase carotenoid isomerase enhanced corresponding enzyme activities, resulting higher lycopene fruit. also stimulated vitamin C synthesis genes (GDP-D-mannose-3', 5'-isomerase, GDP-L-galactose phosphorylase, dehydroascorb-ate reductase, monodehydroascorbate reductase) levels accumulation. health, nutritional at molecular level, favoring production towards sustainable agricultural development.

Language: Английский

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CRISPR/Cas9-edited SPL-CNR quantitatively control tomato fruit ripening

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 330, P. 113073 - 113073

Published: March 12, 2024

Language: Английский

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Maximizing saffron apocarotenoid production in varied tomato fruit carotenoid contexts

The Plant Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Saffron spice owes its commercial appreciation to specific apocarotenoids: crocins, picrocrocin, and safranal. In Crocus sativus, these compounds are biosynthesized from zeaxanthin through oxidative cleavage by the carotenoid dioxygenase 2 (CCD2). Transgenic tomato plants expressing CsCCD2 in fruit, named Tomaffron, accumulate high levels of saffron apocarotenoids despite low substrate availability for CsCCD2. present study, has been introduced into Xantomato; this variety accumulates β-carotene ripe fruit due a combination four mutant alleles. Xantomato Tomaffron genotypes have combined optimize apocarotenoid production. The best transgenic lines accumulated 15 14 times more crocins picrocrocin than alongside fourfold increase compared Xantomato, albeit at cost yield. Segregation mutations carried out find obtaining without adverse effects on Plants harboring high-pigmented 3 (hp3) BETA (B

Language: Английский

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Carotenoids: resources, knowledge, and emerging tools to advance apocarotenoid research

Plant Science, Journal Year: 2024, Volume and Issue: 350, P. 112298 - 112298

Published: Oct. 21, 2024

Language: Английский

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The MdMYB44‐MdTPR1 repressive complex inhibits MdCCD4 and MdCYP97A3 expression through histone deacetylation to regulate carotenoid biosynthesis in apple

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(1), P. 540 - 556

Published: April 25, 2024

SUMMARY Carotenoids are photosynthetic pigments and antioxidants that contribute to different plant colors. However, the involvement of TOPLESS (TPL/TPR)‐mediated histone deacetylation in modulation carotenoid biosynthesis through ethylene‐responsive element‐binding factor‐associated amphiphilic repression (EAR)‐containing transcription factors (TFs) apple ( Malus domestica Borkh.) is poorly understood. MdMYB44 a transcriptional repressor contains an EAR motif. In present study, we used functional analyses molecular assays elucidate mechanisms which MdMYB44‐MdTPR1‐mediated influences apples. We identified two biosynthetic genes, MdCCD4 MdCYP97A3 , were confirmed be involved MdMYB44‐mediated biosynthesis. enhanced β‐branch by repressing expression, whereas suppressed lutein level expression. Moreover, partially interacting with co‐repressor TPR1 motif inhibit expression via deacetylation. Our findings indicate MdTPR1‐MdMYB44 repressive cascade regulates biosynthesis, providing profound insights into basis deacetylation‐mediated plants. These results also provide evidence EAR‐harboring TF/TPL complex plays universal role inhibition gene various

Language: Английский

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Genome-wide analysis of carotenoid cleavage oxygenases and identification of ripening-associated DzNCED5a in durian (Durio zibethinus) fruit

Plant Physiology and Biochemistry, Journal Year: 2023, Volume and Issue: 206, P. 108253 - 108253

Published: Dec. 3, 2023

Language: Английский

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Plant Synthetic Promoters

Published: April 17, 2024

The article features the structure and functions of plant synthetic promoters frequently exercised to precisely regulate complex regulatory routes. composition native together with interacting proteins is presented provide a better understanding tasks associated promoter development. production conferred on relatively small libraries produced generally by basic molecular or genetic engineering methods such as cis-element shuffling domain swapping. Moreover, preparation large-scale supported DNA fragments, directed evolution, machine deep learning methodologies presented. A particularly interesting group are bidirectional forms that enable putative expression up 6–8 genes one element. introduction controlled several after transgenic event strongly decreases frequency problems segregation patterns random integration multiple transgenes. These complications commonly observed during crop development through traditional, multistep transformation constructs containing single gene. Another path solving low complexity homology already tested fragments orthogonal systems composed trans-factors do not occur in nature arise from different species. Their structure, functions, applications rendered article.

Language: Английский

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