CmCYC2d is a Regulator of Leaf Abaxial Curling in Chrysanthemum morifolium

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

ABSTRACT Leaf morphology is crucial for plant photosynthesis and stress adaptation. While CIN‐like TCP transcription factors are well‐known their roles in leaf curling morphogenesis, the function of CYC‐like TCPs development remains largely unexplored. This study identifies CmCYC2d as a key regulator abaxial Chrysanthemum morifolium . Phenotypic analysis revealed that downward observed OX‐ transgenic lines was primarily due to enlargement adaxial epidermal cells. Furthermore, reduction cell number identified significant contributor smaller area these plants. Transcriptome WGCNA analyses highlighted CmSAUR55 potential downstream target CmCYC2d. ChIP‐qPCR, EMSA, LUC assays confirmed directly bound promoter. Additionally, transcriptome data reduced may be mediated by auxin‐related pathways genes such CNR7 The CmCYC2d‐CmSAUR55 module also closely linked enlarged cells sinus, emphasising its role this developmental process. highlights regulatory sheds light on molecular mechanisms underlying chrysanthemum.

Language: Английский

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Integrated analysis of metabolome and transcriptome provides insights into the metabolic adjustments of heteroblastic foliage in Pinus massoniana seedlings

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120684 - 120684

Published: Feb. 13, 2025

Language: Английский

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Four near-complete genome assemblies reveal the landscape and evolution of centromeres in Salicaceae

Genome biology, Journal Year: 2025, Volume and Issue: 26(1)

Published: May 2, 2025

Language: Английский

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DNA methylation profile revealed the dynamically epigenetic regulation of the distinct heteromorphic leaf development in Populus euphratica

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 216, P. 118688 - 118688

Published: May 14, 2024

The heteromorphic leaves of Populus euphratica are typically an adaptive trait in reaction to the dry desert surroundings. involvement DNA methylation plant organ development and response environment is well-established. Nevertheless, there still limited understanding regarding specific regulatory processes governing during formation adjustment P. euphratica. Here, comprehensive landscape for 90 samples across two growth stages was presented first time. In combination with corresponding mRNA transcriptomes, insights into epigenetic regulation were brought light. It observed that linear lanceolate demonstrated increase compared ovate broad-ovate leaves, gradually decreased leaf ripening. Additionally, regulated expression genes related structure function, such as CUS2 Rab-H1b, indicating a mechanism enhancing adaptability. auxin hormone signaling pathway closely linked alterations transcript levels factor tightly by methylation. This research expands knowledge mechanisms involved thus could contribute further studies on evolution

Language: Английский

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The Euphrates Poplar Responses to Abiotic Stress and Its Unique Traits in Dry Regions of China (Xinjiang and Inner Mongolia): What Should We Know?

Genes, Journal Year: 2023, Volume and Issue: 14(12), P. 2213 - 2213

Published: Dec. 14, 2023

At the moment, drought, salinity, and low-temperature stress are ubiquitous environmental issues. In arid regions including Xinjiang Inner Mongolia other areas worldwide, area of tree plantations appears to be rising, triggering growth. Water is a vital resource in agricultural systems countries impacted by aridity salinity. Worldwide efforts reduce quantitative yield losses on Populus euphratica adapting plant production unfavorable conditions have been made response responsiveness increasing control water stress. Although there has much advancement identifying genes that resist abiotic stresses, little known about how plants such as P. deal with numerous stresses. varied riparian can tolerate low temperatures, climate change, variety adaptability abilities. To conduct this review, we gathered all available information throughout Web Science, Chinese National Knowledge Infrastructure, Center for Biotechnology Information impact molecular mechanism evolution gene families at transcription level. The data demonstrated might gradually adapt its stomatal aperture, photosynthesis, antioxidant activities, xylem architecture, hydraulic conductivity endure extreme drought salt Our analyses will give readers an understanding manage family desert trees influence stresses productivity plants. They also knowledge necessary improve biotechnology-based tolerance sustaining quality China’s regions.

Language: Английский

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Comparative genomic analysis of the Growth-Regulating Factors-Interacting Factors (GIFs) in six Salicaceae species and functional analysis of PeGIF3 reveals their regulatory role in Populus heteromorphic leaves

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 28, 2024

Abstract Background The growth-regulating factor-interacting factor ( GIF) gene family plays a vital role in regulating plant growth and development, particularly controlling leaf, seed, root meristem homeostasis. However, the regulatory mechanism of heteromorphic leaves by GIF genes Populus euphratica as an important adaptative trait response to desert environment remains unknown. Results This study aimed identify characterize P. other five Salicaceae species investigate their leaf development. A total 27 were identified characterized across six , pruinose deltoides trichocarpa Salix sinopurpurea suchowensis ) at genome-wide level. Comparative genomic analysis among these suggested that expansion GIFs may be derived from specific whole-genome duplication event after divergence Arabidopsis thaliana . Furthermore, expression data PeGIFs leaves, combined with functional information on indicated development especially containing several cis -acting elements associated By heterologous PeGIF3 wild-type plants (Col-0) atgif1 mutant A. significant difference along medial-lateral axis, increased number cells, observed between overexpressed wild type. Conclusion enhances cell proliferation, thereby resulting central-lateral region leaf. findings not only provide global insights into evolutionary features but also reveal

Language: Английский

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Binding of PtoRAP2.12 to demethylated and accessible chromatin regions in the PtoGntK promoter stimulates growth of poplar

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Summary DNA methylation is an essential epigenetic modification for gene regulation in plant growth and development. However, the precise mechanisms of remain poorly understood, especially woody plants. We employed whole‐genome bisulfite sequencing (WGBS), assays transposase‐accessible chromatin using (ATAC‐seq), RNA‐Seq to investigate regulatory relationships Populus tomentosa treated with inhibitor 5‐azacitidine. Expression‐quantitative trait analysis (eQTM), epigenome‐wide association study (EWAS), joint linkage‐linkage disequilibrium mapping were used explore genes, CRISPR/Cas9 identify role candidate genes. Plant developmental abnormalities occurred when levels substantially reduced. regulated 112 expressed genes via accessibility, which 61 significantly influenced by variation at population level. One methylation‐regulated gene, PtoGntK , was located a major quantitative locus (QTL) poplar growth. Overexpression revealed it affected height stem diameter. The PtoRAP2.12 found bind demethylated accessible region promoter, thereby promoting poplar. This identified key provides insights into

Language: Английский

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The Combined Analysis of the Transcriptome and Metabolome Revealed the Possible Mechanism of Flower Bud Formation in Amorphophallus bulbifer

Agronomy, Journal Year: 2024, Volume and Issue: 14(3), P. 519 - 519

Published: March 2, 2024

The flowering of Amorphophallus bulbifer (A. bulbifer) plays an important role in its reproduction. flowers and leaves A. cannot grow at the same time. However, physiological molecular mechanisms involved flower bud leaf formation are still unclear. In this study, buds early stage growth were used as research materials, transcriptome metabolome analyses carried out, soluble sugar starch contents corms determined. Transcriptome analysis revealed 5542 differentially expressed genes (DEGs) between buds, 3107 which upregulated 2435 downregulated. Enrichment KEGG pathway showed that these differential enriched mainly plant hormone signal transduction, DNA replication fatty acid elongation pathways. A total 5296 significant abundant metabolites screened out by nontargeted metabolomics analysis. functionally classified HMDB, 118 successfully matched, including 17 highly buds. pathways such amino metabolism, isoquinoline alkaloid biosynthesis pyrimidine metabolism. Targeted ABA, ZT iPA significantly greater than those while opposite trend was observed for IAA. results study development regulated acids, starch, ZT, iPA, IAA other hormones. These findings could lead to valuable genetic resources further provide a deeper understanding basis flowering.

Language: Английский

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Structural divergence and phylogenetic relationships of Ajania (Asteraceae) from plastomes and ETS

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: Oct. 10, 2023

Ajania Poljakov, an Asteraceae family member, grows mostly in Asia's arid and semi-desert areas is a significant commercial decorative plant. Nevertheless, the genus' classification has been disputed, evolutionary connections within genus have not thoroughly defined. Hence, we sequenced analyzed Ajania's plastid genomes combined them with ETS data to assess their phylogenetic relationships.We obtained total of six new nine sequences. The whole plastome lengths species sampled ranged from 151,002 bp 151,115 bp, showing conserved structures. Combined publicly available GenBank, constructed datasets reconstruct relationships, detecting nucleoplasmic clashes. Our results reveal affinities Artemisia, Chrysanthemum Stilpnolepis validate early taxonomy reclassification. Some genes low information gene trees topological differences may contributed ambiguous Ajania. There extensive rate heterogeneity genes. psbH ycf2 genes, which are involved photosynthesis ATP transport, under selective pressure. Plastomes diverged, structural aspects plastomes indicate some real connections. We suggest ycf1 as viable DNA barcode because it nucleotide diversity better reflects connections.Our findings reclassification show heterogeneity, genetic variety, This research might provide insights into evolution Ajania, well useful for germplasm innovation enhancement horticultural species.

Language: Английский

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Drought stress-induced the formation of heteromorphic leaves of Populus euphratica Oliv: evidence from gene transcriptome

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: June 7, 2023

Populus euphratica Oliv., a dominant species of arid desert community, grows heteromorphic leaves at different crown positions. Whether are strategy plant adaptation to drought stress is rarely reported. This study sequenced the transcriptome three typical (lanceolate, ovate and broad-ovate leaves) P. , measured their stress. We wanted reveal molecular mechanisms underlying formation leaves. Drought was increased significantly from lanceolate Gene ontology (GO) KEGG enrichment analysis showed that MADs-box gene regulated expression peroxidase (POD) in phenylpropane biosynthetic pathway. The up-regulated chalcone synthase (CHS) activated flavonoid In process leaf shape change, expressions homeodomain leucine zipper (HD-ZIP) among had potential interactions on AUX ABA pathways. Sucrose phosphate (SPS) sucrose (SUS) leaves, resulting consistent change starch content. concluded these resistance-related pathways expressed parallel with genes, thereby inducing Our work provided new insights for plants adapt

Language: Английский

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