PLANT PHYSIOLOGY,
Journal Year:
2024,
Volume and Issue:
196(3), P. 1869 - 1886
Published: Aug. 14, 2024
Abstract
Blotches
in
floral
organs
attract
pollinators
and
promote
pollination
success.
Tree
peony
(Paeonia
suffruticosa
Andr.)
is
an
internationally
renowned
cut
flower
with
extremely
high
ornamental
economic
value.
Blotch
formation
on
P.
petals
predominantly
attributed
to
anthocyanin
accumulation.
However,
the
endogenous
regulation
of
blotch
remains
elusive.
Here,
we
identified
regulatory
modules
governing
anthocyanin-mediated
petals,
which
involves
transcription
factors
PsMYB308,
PsMYBPA2,
PsMYB21.
PsMYBPA2
activated
PsF3H
expression
provide
sufficient
precursor
substrate
for
biosynthesis.
PsMYB21
both
PsFLS
expressions
promoted
flavonol
The
significantly
nonblotch
regions
inhibited
by
competing
biosynthesis
substrates,
while
conversely,
its
low
region
formation.
PsMYB308
PsDFR
directly
prevent
Notably,
a
smaller
area,
decreased
content,
inhibition
structural
gene
were
observed
PsMYBPA2-silenced
opposite
phenotypes
PsMYB308-silenced
PsMYB21-silenced
petals.
Additionally,
interacted
PsbHLH1-3,
their
intensity
target
genes
was
synergistically
regulated
PsMYBPA2-PsbHLH1-3
PsMYB308-PsbHLH1-3
complexes.
also
competitively
bound
PsbHLH1-3
fine-tune
network
overaccumulation
regions.
Overall,
our
study
uncovers
complex
R2R3-MYB
transcriptional
that
governs
providing
insights
into
molecular
mechanisms
underlying
suffruticosa.
Proceedings of the National Academy of Sciences,
Journal Year:
2025,
Volume and Issue:
122(6)
Published: Feb. 4, 2025
Ginkgolides
are
secondary
metabolites
unique
to
Ginkgo
biloba
with
the
potential
prevent
and
treat
cardiovascular
cerebrovascular
diseases.
Although
biosynthetic
pathways
of
ginkgolides
have
been
partly
uncovered,
mechanism
regulating
their
biosynthesis
is
still
largely
unknown.
Here,
using
multiomic
genetic
analyses,
we
report
identification
a
transcription
factor,
named
ETHYLENE
RESPONSE
FACTOR
ASSOCIATED
WITH
GINKGOLIDE
BIOSYNTHESIS
(GbEAG),
as
critical
regulator
ginkgolide
biosynthesis.
GbEAG
highly
expressed
in
roots
G.
biloba,
its
expression
significantly
induced
by
methyl
jasmonate
(MeJA).
Ginkgolide
content
was
increased
overexpressing
“cut-dip-regeneration”
system.
positively
regulates
directly
binding
GCC-boxes
promoter
regions
genes
involved
ginkgolides,
such
ISOPENTENYL
DIPHOSPHATE
ISOMERASE
(
GbIDI
)
CYTOCHROME
P450
7005C3
GbCYP7005C3
).
mediates
jasmonic
acid
(JA)-activated
synthesis
through
direct
interaction
JASMONATE
ZINC-FINGER
INFLORESCENCE
MERISTEM
DOMAIN
3
(GbJAZ3)
repressor.
Importantly,
also
found
that
central
light-response
ELONGATED
HYPOCOTYL
5
(GbHY5)
light
induction
G-box
promoter.
Our
findings
provide
mechanistic
insights
into
coordinated
regulation
via
JA
signals,
shed
on
develop
ginkgolide-rich
varieties
molecular
breeding
gene
editing.
Plants,
Journal Year:
2024,
Volume and Issue:
13(11), P. 1557 - 1557
Published: June 4, 2024
The
plant
hormone
jasmonic
acid
plays
an
important
role
in
growth
and
development,
participating
many
physiological
processes,
such
as
disease
resistance,
stress
organ
root
growth,
flowering.
With
the
improvement
living
standards,
people
have
higher
requirements
regarding
quality
of
vegetables.
However,
during
process
vegetables,
they
are
often
attacked
by
pests
diseases
undergo
abiotic
stresses,
resulting
their
restriction
decreases
yield
quality.
Therefore,
found
ways
to
regulate
vegetable
crops.
In
recent
years,
addition
that
JA
response
it
has
been
a
regulatory
effect
on
crop
this
study
aims
review
accumulation
patterns
various
processes
its
potential
development
formation,
well
underlying
molecular
mechanisms.
information
provided
manuscript
sheds
new
light
improvements
Frontiers in Plant Science,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 15, 2025
Mentha
arvensis
L.
(
M.
)
is
an
aromatic
plant
of
the
genus,
renowned
for
its
medicinal
and
economic
importance.
The
primary
components
essential
oils
(EOs)
are
monoterpenoids,
synthesized
stored
in
peltate
glandular
trichomes
(PGTs).
In
general,
EO
content
relatively
low.
Methyl
jasmonate
(MJ)
has
been
reported
as
effective
elicitor
terpenoid
biosynthesis
plants,
but
specific
mechanisms
underlying
MJ’s
influence
on
remain
unclear.
this
study,
exogenous
application
MJ
significantly
increased
content,
yield,
PGT
density
a
dose-dependent
manner.
At
5
mM
dose,
peaked,
with
increases
71.20%
53.69%,
respectively.
Gas
chromatography-mass
spectrometry
(GC-MS)
analysis
indicated
that,
treatment
did
not
alter
types
or
relative
proportions
.
However,
L-menthol
decreased
slightly
by
7.90%
under
treatment.
Transcriptome
identified
4,659
differentially
expressed
genes
(DEGs)
MJ-treated
leaves.
KEGG
enrichment
revealed
that
“Monoterpenoid
biosynthesis”
was
among
most
enriched
metabolic
pathways.
Key
involved
jasmonic
acid
(JA)
signaling
JAZs
MYCs
monoterpenoid
GPPSs
,
LSs
L3Hs
IPRs
were
up-regulated.
Co-expression
analysis,
promoter
binding
element
weighted
gene
co-expression
network
(WGCNA)
transcription
factors
(TFs)
such
AP2/ERF,
WRKY,
MYB,
bHLH
play
crucial
roles
regulating
MJ-mediated
biosynthesis.
Several
key
candidate
TFs
potentially
identified.
These
findings
provide
valuable
insights
into
molecular
accumulation
genus.
PeerJ,
Journal Year:
2025,
Volume and Issue:
13, P. e18914 - e18914
Published: Feb. 10, 2025
Background
Chinese
herbal
medicine
(CHM)
is
a
fundamental
component
of
traditional
medical
practice,
offering
rich
source
natural
remedies
with
significant
therapeutic
potential.
However,
the
scarcity
active
ingredients
and
complex
extraction
procedures
present
substantial
challenges
to
their
widespread
clinical
application.
This
review
aims
address
this
gap
by
exploring
potential
modern
biotechnological
advancements
in
enhancing
biosynthesis
these
valuable
compounds.
Methodology
The
study
takes
comprehensive
approach,
delving
into
chemical
composition
CHM’s
elucidating
biosynthetic
pathways
molecular
regulatory
mechanisms.
Additionally,
it
surveys
recent
progress
methodologies
evaluates
engineering
strategies
aimed
at
synthetic
production.
multifaceted
analysis
forms
foundation
for
examining
role
biology
augmenting
ingredient
synthesis.
Results
Our
examination
provides
insights
intricate
governing
formation
ingredients,
as
well
networks
that
underlie
processes.
Furthermore,
highlights
techniques,
demonstrating
ability
streamline
enhance
isolation
Engineering
approaches
production,
including
metabolic
tools,
are
assessed
overcome
limitations
scale
up
Conclusions
By
integrating
from
biosynthesis,
regulation,
methodologies,
biology,
establishes
robust
theoretical
framework
production
ingredients.
proposed
practical
guidance
aim
facilitate
broader
utilization
while
promoting
sustainability
accessibility
within
invaluable
medicinal
heritage.
