Published: Jan. 1, 2024
Language: Английский
Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 87, P. 103139 - 103139
Published: April 30, 2024
Language: Английский
Citations
5
Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 22, 2025
With the advancement of plant synthetic biology, complex genetic engineering circuits are being developed, which require more diverse regulatory elements (promoters) to operate. Constitutive promoters widely used for such gene projects, but list strong, constitutive with strength surpassing promoter, CaMV35S, is limited. In this work, we attempted increase promoter library by developing efficient suitable high-level expression. To do that, selected three strong pararetroviral-based from Mirabilis mosaic virus (MMV), Figwort (FMV), and Horseradish latent (HRLV) rationally designed combined their elements. We then tested newly developed in Nicotiana benthamiana found a highly active tri-hybrid MuasFuasH17 (MFH17). further these generating random mutant DNA shuffling techniques an attempt change/improve MFH17 promoter. evaluated activity Oryza sativa seedlings studied effect as-1 present it. Finally, efficacy tissue specificity planta transgenic tabacum Arabidopsis thaliana plants it driving throughout tissues. Overall, conclude that tripartite constitutive, dual-species (dicot monocot) expressing can be valuable addition biology.
Language: Английский
Citations
0
The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)
Published: March 1, 2025
SUMMARY Plants are a valuable source of diverse specialized metabolites with numerous applications. However, these compounds often produced in limited quantities, particularly under unfavorable ecological conditions. To achieve sufficient levels target metabolites, alternative strategies such as pathway engineering heterologous systems like microbes (e.g., bacteria and fungi) or cell‐free can be employed. Another approach is plant engineering, which aims to either enhance the native production original reconstruct model system. Although increasing metabolite promising strategy, plants exotic pose significant challenges for genetic manipulation. Effective requires comprehensive prior knowledge genes enzymes involved, well precursor, intermediate, branching, final metabolites. Thus, thorough elucidation biosynthetic closely linked successful metabolic host systems. In this review, we highlight recent advances engineering. We focus on efforts engineer complex, multi‐step pathways that require expression at least eight transient three stable transformation. Reports complex stably transformed remain relatively scarce. discuss major hurdles overcoming them, followed by an overview achievements, challenges, solutions reconstitution through Recent including computer‐based predictions offer platforms sustainable plants.
Language: Английский
Citations
0
BioDesign Research, Journal Year: 2025, Volume and Issue: unknown, P. 100007 - 100007
Published: March 1, 2025
Language: Английский
Citations
0
The Plant Cell, Journal Year: 2024, Volume and Issue: 36(5), P. 1183 - 1185
Published: March 11, 2024
to fill a gap in the rapidly progressing areas of
Language: Английский
Citations
2
Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15
Published: Aug. 6, 2024
Promoters are one of the most important components for many gene-based research as they can fine-tune precise gene expression. Many unique plant promoters have been characterized, but strong with dual expression in both monocot and dicot systems still lacking. In this study, we attempted to make such a promoter by combining specific domains from monocot-infecting pararetroviral-based sugarcane bacilliform virus (SCBV) banana streak (BSV) dicot-infecting mirabilis mosaic (MMV). The generated chimeric promoters, MS, SM, MB, BM, were tested further validated transgenic tobacco plants. We found that developed species-specific (monocot or dicot), which depended on their respective core (CP) region. Furthermore, knowledge, deletion-hybrid evaluated, led development dual-expressing promoter, MSD3, high efficiency (GUS GFP reporter genes) rice, pearl millet, conclude MSD3 be an genetic tool will valuable biology application.
Language: Английский
Citations
1
PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(1), P. 1 - 3
Published: March 28, 2024
Language: Английский
Citations
0
Cell chemical biology, Journal Year: 2024, Volume and Issue: 31(10), P. 1745 - 1754
Published: Aug. 29, 2024
Language: Английский
Citations
0
Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 90, P. 103211 - 103211
Published: Sept. 27, 2024
Language: Английский
Citations
0
Published: Jan. 1, 2024
Language: Английский
Citations
0