Potentiation of Catalase-Mediated Plant Thermotolerance by N-Terminal Attachment of Solubilizing/Thermostabilizing Fusion Partners DOI Open Access

Guoqing Xie,

Yanrong Huang,

Di Hu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12181 - 12181

Published: Nov. 13, 2024

Catalase (CAT) plays a crucial role in plant responses to environmental stresses and maintaining redox homeostasis. However, its putative heat lability might compromise activity function, thus restricting thermotolerance. Herein, we verified Arabidopsis CAT3 was of poor thermostability that then engineered by fusion expression Escherichia coli. We found our selected partners, three hyperacidic mini-peptides the short rubredoxin from hyperthermophile Pyrococcus furiosus, were commonly effectual enhance solubility enlarge improvement on tolerance E. coli yeast. Most importantly, this finding also achievable plants. Fusion could magnify CAT3-mediated thermotolerance tobacco. Under stress, transgenic lines expressing fusions generally outperformed native which turn surpassed wild-type tobacco, terms seed germination, seedling survival, recovery growth, protection chlorophyll membrane lipids, elimination H2O2, as well mitigation cell damage leaves roots. Moreover, revealed introduced or seemed solely responsible for enhanced Prospectively, strategy would be applicable other proteins intrinsic instability provide an alternative biotechnological route ameliorating tolerance.

Language: Английский

The OsEBF1-OsEIL5-OsPP91 module regulates rice heat tolerance via ubiquitination and transcriptional activation DOI Creative Commons
Jianping Liu,

Ke Wang,

Guining Wang

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: 44(2), P. 115271 - 115271

Published: Feb. 1, 2025

Highlights•The F box protein OsEBF1 negatively regulates rice thermotolerance•OsEBF1 interacts with and degrades the transcription factor OsEIL5•OsEIL5 confers tolerance to heat stress at seedling reproductive stages•As target of OsEIL5, OsPP91 is indispensable for toleranceSummaryUnderstanding regulatory mechanisms underlying plant response important developing climate-resilient crops, including (Oryza sativa). Here, we report that one member ETHYLENE INSENSITIVE3-LIKE family, positively stages. OsEIL5 directly binds promoter (encoding type 2C phosphatase) activates its expression. required thermotolerance, overexpressing in oseil5-1 partially rescues sensitivity. The it through ubiquitination pathway, resulting reduction expression ultimately weakening tolerance. Knocking out EBF1R13 line reduces extremely high Taken together, our work uncovers a mechanism finely thermotolerance OsEBF1-OsEIL5-OsPP91 module posttranslational transcriptional levels.Graphical abstract

Language: Английский

Citations

1
Functional analysis of <i>MeFER4</i> in regulating ROS homeostasis during abiotic stress responses in <i>Arabidopsis</i> DOI

Mingjuan Zheng,

Jianjun Li, Chenyu Lin

et al.

Tropical Plants, Journal Year: 2025, Volume and Issue: 4(1), P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

0
CPK1‐HSP90 phosphorylation and effector XopC2–HSP90 interaction underpin the antagonism during cassava defense‐pathogen infection DOI
Yunxie Wei, Binbin Zhu, Y. Zhang

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 242(6), P. 2734 - 2745

Published: April 6, 2024

Summary Cassava is one of the most important tropical crops, but it seriously affected by cassava bacteria blight (CBB) caused bacterial pathogen Xanthomonas phaseoli pv manihotis ( Xam ). So far, how infects and host defends during pathogen–host interaction remains elusive, restricting prevention control CBB. Here, illustration HEAT SHOCK PROTEIN 90 kDa (MeHSP90.9) interacting proteins in both revealed dual roles MeHSP90.9 cassava– interaction. On hand, calmodulin‐domain protein kinase 1 (MeCPK1) directly interacted with to promote its phosphorylation at serine 175 residue. The improved transcriptional activation clients (SHI‐RELATED SEQUENCE (MeSRS1) MeWRKY20) downstream target genes avrPphB Susceptible 3 MePBS3 ) N‐aceylserotonin O‐methyltransferase 2 MeASMT2 )) immune responses. other outer C2 (XopC2) physically associated inhibit MeCPK1 corresponding MeCPK1, so as repress responses infection. In summary, these results provide new insights into genetic improvement disease resistance extend our understanding cassava–bacterial

Language: Английский

Citations

1
Potentiation of Catalase-Mediated Plant Thermotolerance by N-Terminal Attachment of Solubilizing/Thermostabilizing Fusion Partners DOI Open Access

Guoqing Xie,

Yanrong Huang,

Di Hu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12181 - 12181

Published: Nov. 13, 2024

Catalase (CAT) plays a crucial role in plant responses to environmental stresses and maintaining redox homeostasis. However, its putative heat lability might compromise activity function, thus restricting thermotolerance. Herein, we verified Arabidopsis CAT3 was of poor thermostability that then engineered by fusion expression Escherichia coli. We found our selected partners, three hyperacidic mini-peptides the short rubredoxin from hyperthermophile Pyrococcus furiosus, were commonly effectual enhance solubility enlarge improvement on tolerance E. coli yeast. Most importantly, this finding also achievable plants. Fusion could magnify CAT3-mediated thermotolerance tobacco. Under stress, transgenic lines expressing fusions generally outperformed native which turn surpassed wild-type tobacco, terms seed germination, seedling survival, recovery growth, protection chlorophyll membrane lipids, elimination H2O2, as well mitigation cell damage leaves roots. Moreover, revealed introduced or seemed solely responsible for enhanced Prospectively, strategy would be applicable other proteins intrinsic instability provide an alternative biotechnological route ameliorating tolerance.

Language: Английский

Citations

0