CRISPR/Cas9-based mutant library screening: the discovery of novel genes regulating immune responses in cotton and rice DOI Creative Commons
Sang Ryeol Park, Seungmin Son

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 14, 2024

The environmental conditions play a crucial role in determining crop yield, which is essential for ensuring food and nutritional security. However, rapid climate change exacerbating global stress, leading to severe biotic pressures on crops. Therefore, enhancing resilience pathogens has become one of the most pressing challenges humanity. Large-scale mutant library screening efficient strategy identifying numerous genes associated with specific traits. revolutionary CRISPR/Cas9 system ushered new era construction library. its application plants been relatively scarce compared mammals, largely due accessibility. Fortunately, several research groups have recently developed CRISPR/Cas9-based libraries, successfully variety involved immunity. In this review, we present an overview discussion studies that generated significant results through use identify novel resistance stresses within field plant research.

Language: Английский

Transcription factors – insights into abiotic and biotic stress resilience and crop improvement DOI Creative Commons
Roopali Bhoite,

Olive Onyemaobi,

Tanushree Halder

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100434 - 100434

Published: Jan. 1, 2025

Language: Английский

Citations

4
The Role of Genetic Resistance in Rice Disease Management DOI Open Access
Andrews Danso Ofori, Tengda Zheng, John Kwame Titriku

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 956 - 956

Published: Jan. 23, 2025

Rice (Oryza sativa) is a crucial staple crop for global food security, particularly in Asia. However, rice production faces significant challenges from various diseases that can cause substantial yield losses. This review explores the role of genetic resistance disease management, focusing on molecular mechanisms underlying plant–pathogen interactions and strategies developing resistant varieties. The paper discusses qualitative quantitative resistance, emphasizing importance (R) genes, defense-regulator trait loci (QTLs) conferring broad-spectrum resistance. Gene-for-gene relationships rice–pathogen are examined, Xanthomonas oryzae pv. Magnaporthe oryzae. also covers recent advancements breeding techniques, including marker-assisted selection, engineering, genome editing technologies like CRISPR-Cas. These approaches offer promising avenues enhancing while maintaining potential. Understanding exploiting durable disease-resistant varieties, essential ensuring sustainable security face evolving pathogen threats changing environmental conditions.

Language: Английский

Citations

0
CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops DOI Creative Commons
Navjot Kaur, Muslim Qadir, Dali Vilma Francis

et al.

Frontiers in Genome Editing, Journal Year: 2025, Volume and Issue: 7

Published: March 18, 2025

Climate change is a global concern for agriculture, food security, and human health. It affects several crops causes drastic losses in yield, leading to severe disturbances the economy, environment, community. The consequences on important staple crops, such as rice, maize, wheat, will worsen create insecurity across globe. Although various methods of trait improvements are available being used, clustered regularly interspaced short palindromic repeats CRISPR-associated protein 9 (CRISPR/Cas9) mediated genome manipulation have opened new avenue functional genomics crop improvement. This review discuss progression improvement from conventional breeding advanced editing techniques how CRISPR/Cas9 technology can be applied enhance tolerance main cereal (wheat, maize) against any harsh climates. CRISPR/Cas endonucleases their derived genetic engineering tools possess high accuracy, versatile, more specific, easy design, climate-smart or resilient combat survive environments. CRISPR/Cas9-mediated approach has been make them climate resilient. review, supported by bibliometric analysis recent literature, highlights potential target genes/traits addresses significance gene technologies tackling vulnerable effects major maize.

Language: Английский

Citations

0
Genome-Wide Identification, Characterization, and Expression Analysis of the NAC Transcription Factor Family in Sweet Cherry (Prunus avium L.) DOI Creative Commons
Feng An, Xin Yin,

Kaibire Jueraiti

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(8), P. 1201 - 1201

Published: April 12, 2025

The NAC (NAM, ATAF1/2, and CUC2) family is one of the largest plant-specific transcription factor families, playing a crucial role in adaptation to abiotic stresses. However, gene sweet cherry (Prunus avium L.) remains poorly understood. In this study, we identified 130 genes (PaNAC) from genome, which were unevenly distributed across eight chromosomes. Phylogenetic analysis classified PaNACs into 21 distinct groups, including 2 cherry-specific groups. Comparative revealed significant variations proportions, exon–intron structures, motif compositions among different Furthermore, cis-element suggested potential roles regulating plant growth, development, hormone signaling, stress responses. Transcriptomic data tissue-specific expression patterns for several PaNAC genes. qRT-PCR further confirmed that selected responsive various stresses Gisela 6, widely used hybrid rootstock production shares high sequence similarity with P. avium. These findings provide valuable insights future research on functional characteristics responses cherry.

Language: Английский

Citations

0
Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics DOI Creative Commons

Eyob Kassaye Wolella,

Cheng Zhen, Mengyuan Li

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(10), P. 1492 - 1492

Published: May 16, 2025

Rice (Oryza sativa L.) is a stable food for over half of the world population, contributing 50–80% daily calorie intake. The completion rice genome sequencing marks significant milestone in understanding functional genomics, yet systematic identification gene functions remains bottleneck improvement. Large-scale mutant libraries which genes are lost or gained (e.g., through chemical/physical treatments, T-DNA, transposons, RNAi, CRISPR/Cas9) have proven to be powerful tools linking genotypes phenotypes. So far, using different mutagenesis approaches, million lines been established and about 5–10% predicted identified due high demands labor low-throughput utilization. DNA-barcoding-based large-scale offers unprecedented precision scalability genomics. This review summarizes loss-of-function gain-of-function library development approaches emphasizes integration DNA barcoding pooled analysis. Unique barcodes can tagged transposons/retrotransposons, constructs, miRNA/siRNA, gRNA, cDNA, allowing pooling analysis assignment that cause phenotype alterations. In addition, high-throughput phenotyping OMICS technologies accelerate functions.

Language: Английский

Citations

0
A NAC transcription factor MNAC3‐centered regulatory network negatively modulates rice immunity against blast disease DOI
Hui Wang, Yan Bi, Yuqing Yan

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(9), P. 2017 - 2041

Published: July 2, 2024

ABSTRACT NAC transcription factors (TFs) are pivotal in plant immunity against diverse pathogens. Here, we report the functional and regulatory network of MNAC3, a novel TF, rice immunity. transcriptional activator, negatively modulates blast bacterial leaf blight diseases pathogen‐associated molecular pattern (PAMP)‐triggered immune responses. MNAC3 binds to CACG cis ‐element activates immune‐negative target genes OsINO80 , OsJAZ10 OsJAZ11 . The negative function depends on its downstream such as interacts with immunity‐related OsPP2C41 (a protein phosphatase), ONAC066 TF), OsDjA6 DnaJ chaperone). attenuate activity, while promotes it. Phosphorylation at S163 is critical for functions OsPP2C41, which plays positive roles resistance chitin‐triggered responses, dephosphorylates suppressing activity promoting translocation from nucleus cytoplasm. These results establish MNAC3‐centered attenuating rice. Together, these findings deepen our understanding mechanisms offer strategy genetic improvement disease resistance.

Language: Английский

Citations

3
Functions of transcription factor superfamilies in rice immunity DOI Creative Commons
Sang Ryeol Park, Yu-jeong Jeong, Seungmin Son

et al.

The Crop Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

Citations

2
Pangenome characterization and analysis of the NAC gene family reveals genes for Sclerotinia sclerotiorum resistance in sunflower (Helianthus annuus) DOI Creative Commons
Yan Lü, Dongqi Liu, Xiangjiu Kong

et al.

BMC Genomic Data, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 1, 2024

Sunflower (Helianthus annuus) is one of the most important economic crops in oilseed production worldwide. The different cultivars exhibit variability their resistance genes. NAC transcription factor (TF) family plays diverse roles plant development and stress responses. With completion H. annuus genome sequence, entire complement genes coding for NACs has been identified. However, reference a single individual cannot cover all genetic information species.

Language: Английский

Citations

1
OsWRKY9 is Involved in Transcriptional Regulatory Cascade Enhancing Broad-Spectrum Disease Resistance DOI
Giha Song, Sang Ryeol Park, Yu-jeong Jeong

et al.

Journal of Plant Biology, Journal Year: 2024, Volume and Issue: 67(5), P. 399 - 408

Published: Sept. 17, 2024

Language: Английский

Citations

0
CRISPR/Cas9-based mutant library screening: the discovery of novel genes regulating immune responses in cotton and rice DOI Creative Commons
Sang Ryeol Park, Seungmin Son

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 14, 2024

The environmental conditions play a crucial role in determining crop yield, which is essential for ensuring food and nutritional security. However, rapid climate change exacerbating global stress, leading to severe biotic pressures on crops. Therefore, enhancing resilience pathogens has become one of the most pressing challenges humanity. Large-scale mutant library screening efficient strategy identifying numerous genes associated with specific traits. revolutionary CRISPR/Cas9 system ushered new era construction library. its application plants been relatively scarce compared mammals, largely due accessibility. Fortunately, several research groups have recently developed CRISPR/Cas9-based libraries, successfully variety involved immunity. In this review, we present an overview discussion studies that generated significant results through use identify novel resistance stresses within field plant research.

Language: Английский

Citations

0