Cytokinin mediates age‐dependent drought response by regulating the removal reactive oxygen species in apple

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(4)

Published: Feb. 1, 2025

SUMMARY Plants exhibit age‐dependent drought responses during juvenile‐to‐adult phase transition. However, the specific regulatory molecular mechanisms remain unknown. In this study, juvenile apple plants exhibited better tolerance than adult because of changes in root vitality, cytokinin (CK) levels, and redox status roots. The study uncovers CYTOKININ OXIDASE/DEHYDROGENASE5 ( MdCKX5 ) as a negative regulator Silencing caused CK accumulation thereby enhancing by increasing vitality preventing reactive oxygen species. contrast, overexpression reduced Yeast one‐hybrid, dual‐luciferase electrophoretic mobility shift assays revealed that transcription factor MdSPL1 directly binds to promoters repressing its expression. Overexpression roots increased content tolerance. Further analysis MdMYB23, positive CK‐responsive gene, interacts with alleviate repression expression MdSPL1. general, stress significantly downregulated MdMYB23 , activating MdSPL1‐mediated releasing This phenomenon led enhanced levels. lost capacity activate cascade. These findings provide new insights into CK‐mediated vegetative change apples.

Language: Английский

Sorbitol mediates age-dependent changes in apple plant growth strategy through gibberellin signaling

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(8)

Published: July 11, 2024

Abstract Plants experience various age-dependent changes during juvenile to adult vegetative phase. However, the regulatory mechanisms orchestrating remain largely unknown in apple (Malus domestica). This study showed that tissue-cultured plants at juvenile, transition, and phase exhibit their plant growth, photosynthetic performance, hormone levels, carbon distribution. Moreover, this identified an gene, sorbitol dehydrogenase (MdSDH1), a key enzyme for catabolism, highly expressed apple. Silencing MdSDH1 significantly decreased growth GA3 levels. exogenous rescued reduced phenotype of TRV-MdSDH1. Biochemical analysis revealed MdSPL1 interacts with MdWRKY24 synergistically enhance repression on MdSDH1, thereby promoting accumulation change. Exogenous application indicated promotes transcription MdWRKY24. Notably, MdSPL1-MdWRKY24 module functions as repressor regulate GA-responsive Gibberellic Acid-Stimulated Arabidopsis (MdGASA1) expression, leading shift from quick slow-growth strategy. These results reveal pivotal role controlling improving our understanding change

Language: Английский