Gene Expression Dynamics of Sugar Metabolism and Accumulation During Fruit Ripening in Camellia drupifera

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 817 - 817

Published: March 5, 2025

Camellia drupifera, a valuable woody oil crop, holds significant ecological, economic, and medicinal importance. Its seed maturation involves intricate physiological changes, particularly the interplay between biosynthesis sugar metabolism. This study investigates accumulation expression dynamics of metabolism-related unigenes during three key developmental stages C. drupifera fruit: nutrient synthesis stage (NS), fat (FA), (MS). The findings reveal distinct differences in content regulatory mechanisms across stages. NS emerges as critical period for metabolism, characterized by peak levels soluble sugars fructose alongside significantly elevated unigenes. correlation sucrose gene suggests crucial role carbohydrates fruit maturation. Transcriptomic analysis identified differentially expressed (DEGs) metabolism pathways, which qRT-PCR further validated. These results offer novel insights into molecular regulating development. At same time, it provides theoretical basis genetic improvement effective utilization other crops, supporting their broader agricultural industrial applications.

Language: Английский

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Re-sequencing and physiological analysis reveals the differences in genetic variation and sugar metabolism between ‘Cuihong’ plum (Prunus salicina) and its early-ripening mutant ‘Hongyun’ plum

Scientia Horticulturae, Journal Year: 2025, Volume and Issue: 343, P. 114079 - 114079

Published: March 1, 2025

Language: Английский

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The Impact of the Natural Grass-Growing Model on the Development of Korla Fragrant Pear Fruit, as Well as Its Influence on Post-Harvest Sugar Metabolism and the Expression of Key Enzyme Genes Involved in Fruit Sugar Synthesis

Agriculture, Journal Year: 2025, Volume and Issue: 15(7), P. 792 - 792

Published: April 7, 2025

In this study, the effects of natural grass cultivation and clear on physiological characteristics Korla fragrant pear during fruit development storage were investigated, providing a scientific basis for high-quality cultivation. Sugar components, enzyme activities, gene expression levels in pulp peel comprehensively analyzed storage. A classification model was constructed using machine learning algorithms (RF, KNN, SVM), particle swarm optimization (PSO) employed to identify key factors. The results showed that significantly enhanced sugar accumulation fruits, particularly at 120 150 days after flowering, with fructose content increasing by 9.09 mg·g−1 12.59 mg·g−1, respectively, glucose also rising significantly. Additionally, promoted relative GK, PFK, FK genes pulp. During storage, activities group consistently higher than those across different periods, PFK activity being 23.73 U/L identified glyceraldehyde kinase, phosphofructokinase, fructokinase as factors influencing content. significant negative correlation observed between phosphofructokinase content, though relationship requires further investigation. This study elucidates regulatory mechanism which methods affect quality through photosynthetic product distribution. Our findings provide critical foundation are expected advance efficient industry, helping farmers improve both income.

Language: Английский

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Transcriptomic Profiling Uncovers Molecular Basis for Sugar and Acid Metabolism in Two Pomegranate (Punica granatum) Varieties

Foods, Journal Year: 2025, Volume and Issue: 14(10), P. 1755 - 1755

Published: May 15, 2025

Soluble sugars and organic acids constitute the primary flavor determinants in fruits elucidating their metabolic mechanisms provides crucial theoretical foundations for fruit breeding practices food industry development. Through integrated physiological transcriptomic analysis of pomegranate varieties 'Sharp Velvet' with high acid content 'Azadi' low content, this study demonstrated that differences between two were mainly caused by citric rather than soluble sugar content. Transcriptome profiling identified 11 candidate genes involved metabolism, including three associated metabolism (FBA1, SS, SWEET16) eight linked to (ADH1, GABP1, GABP2, GABP3, GABP4, ICL, ME1, PDC4). These data indicated stemmed from regulation degradation pathway, which relies on γ-aminobutyric (GABA) branch isocitric lyase (ICL) pathway. Citric accumulation was driven fluxes vesicular storage capacity. Weighted gene co-expression network (WGCNA) uncovered a significant module (r = -0.72) predicted six core transcriptional regulators (bHLH42, ERF4, ERF062, WRKY6, WRKY23, WRKY28) within network. Notably, bHLH42, WRKY28 showed positive correlations whereas WRKY23 negative correlations. Our findings provide comprehensive insights into genetic architecture governing homeostasis pomegranate, offering both novel mechanistic understanding acidity valuable molecular targets precision quality traits.

Language: Английский

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Sugar Accumulation Patterns and Transcriptome Analysis during the Developmental Stage of Small-Fruit Watermelon (Citrullus lanatus L.)

Agronomy, Journal Year: 2024, Volume and Issue: 14(7), P. 1544 - 1544

Published: July 16, 2024

The sugar content in watermelon significantly influences its flavor. To understand the accumulation pattern small-fruited and identify candidate genes involved synthesis conversion, we measured (sucrose, fructose, glucose) at five developmental stages: 10d, 16d, 22d, 28d, 34d post-pollination. Two varieties with largest difference 28d post-pollination (high-sugar G38-28 low-sugar 482276-28) were selected for transcriptome sequencing. Differentially expressed (DEGs) validated using RT-qPCR. Additionally, contents of three commercial (‘Su Meng NO.5’, ‘Su NO.6’, NO.7’) their parents compared stages. Results showed glucose fructose levels peaked between 22d followed by a decrease, while sucrose continuously increased. F1 hybrids exhibited trends similar to paternal parent maternal parent. Transcriptome sequencing identified 9337 DEGs (5072 upregulated 4265 downregulated). Gene Ontology analysis highlighted overrepresentation categories such as pectinase oxidoreductase activity. KEGG 12 conversion pathways, including phenylpropanoid biosynthesis pentose glucuronate interconversions. RT-qPCR validation corroborated data. These findings explain distinct patterns 482276-28 transcriptional level, offering insights genetic breeding regulation key sugar-related watermelon.

Language: Английский

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Expression of the polyphenol oxidase gene MdPPO7 is modulated by MdWRKY3 to regulate browning in sliced apple fruit

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 197(1)

Published: Nov. 13, 2024

Abstract Browning is a pervasive problem in horticultural products, substantially diminishing the appearance, flavor, and nutritional value of fruit, including important fruits like apple (Malus × domestica Borkh.). In this study, we compared physiological characteristics browning-resistant line ‘Rb-18’ with susceptible variety ‘Fuji’ found that polyphenol oxidase (PPO) enzyme activity phenolic content were significantly lower than those ‘Fuji’. addition, PPO showed stronger affinity for its substrate, catechol, to ‘Rb-18’. Through transcriptome RT-qPCR analyses, MdPPO7 expression was identified as contributing flesh browning after cutting. Subsequent fruit injection stable genetic transformation gene into calli determined syringic acid, procyanidin, phloridzin, chlorogenic gallic catechin, caffeic act catalytic substrates process involved browning. Furthermore, luciferase reporter, yeast 1-hybrid, β-glucuronidase reporter assays ChIP-qPCR analysis demonstrated WRKY transcription factor (MdWRKY3) binds promoter region (MdPPO7) positively regulates promote This study provides insights molecular regulatory mechanisms fresh-cut apples theoretical basis generation high-quality germplasm resources.

Language: Английский

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MpYABBY2 promotes ABA accumulation and influences MpMYB11/MpbHLH79 complex to regulate anthocyanin biosynthesis in Malus ‘Pinkspire’ fruits under cold stress

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 222, P. 113341 - 113341

Published: Dec. 6, 2024

Language: Английский

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