Celastrol binds to pyruvate kinase and imitates the metabolic reprogramming of ATP DOI Creative Commons
Xuan Zhang, Xudong Jiang, Xiaobin Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Summary ATP is the final product of glycolysis and TCA cycle. However, counter-regulation glycosis by poorly understood. Here we show that analog celastrol (CLT) binds to binding pocket on pyruvate kinase PKM (PKM) while inducing allosteric regulation PKM. Quantum calculation predicts hydrogen bond formation between CLT asparagine. Liquid chromatography-mass spectrometry further identifies as CLT’s target. The inhibition stronger longer for weak short ATP. Notably, CLT-PKM interaction perfectly underlies hypoglycemic effects forming glucose carbon flux U turn before Besides, allostery induces a tricarboxylic acid (TCA) vortex which could promote amino lipid degradation energy compensation, leading significant weight loss. Additionally, exerts efficient antioxidant altering strengthen pentose phosphate pathway. Consequently, vividly reproduces ATP-PKM named “ATP resistance” in diabetes pathogenesis.

Language: Английский

Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties DOI Open Access
Xiaoyu Tang, Mingxin Huang, Lijun Deng

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 803 - 803

Published: Jan. 18, 2025

Late-maturing hybrid citrus is a significant fruit that combines the best traits of both parents and highly prized for its unique flavor. Not only can organic acids alter flavor pulp, but they are also essential cellular metabolism, energy conversion, maintaining acidbase balance in plant tissues. Although play key role quality formation fruits, there still insufficient research on metabolic processes late-maturing varieties. In this study, three varieties with different acidity levels, namely ‘Huangjinjia’ (HJ), ‘Kiyomi’ (QJ), ‘Harumi’ (CJ), were selected to systematically investigate regulation mechanism through transcriptome sequencing technology, combined physiological biochemical analyses. This study revealed gene expression differences related acid synthesis degradation. Through profiling, several genes closely associated metabolism identified, preliminary network was constructed. The results showed pathways between growth stages fruit. Specifically, HJ had higher TA content than QJ CJ, primarily due significantly citric malic contents compared other two Further analysis four modules high correlation levels major fruits. involved these synthesis, degradation, transport. Additionally, we identified (AS1, BZP44, COL4, TCP4, IDD10, YAB2, GAIPB) might be metabolism. functions could have impact changes enzymes provides foundation exploring intrinsic mechanisms regulating fruits contributes functional molecular design high-quality

Language: Английский

Citations

1
Mutagenesis of AcSQBP9 in kiwifruit results in reduction of malate via alteration of the expression of a plastidial malate dehydrogenase DOI Open Access

Tonghui Qi,

Y. Q. Huang,

Jiahui Deng

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(5)

Published: March 1, 2025

Organic acids are major contributors to the flavor of fleshy fruits. In kiwifruit, Al-ACTIVATED MALATE TRANSPORTER gene (AcALMT1) is key accumulation citrate, while factors driving malate metabolism remain largely unknown. During kiwifruit (Actinidia chinensis cv "Hongyang") development, a rapid decline content was observed between 6 and 12 weeks after full bloom (WAFB), which studied using RNA-seq analysis. Co-expression network analysis indicated that expression chloroplast localized AcPNAD-MDH1 (Plastid-Localized NAD-Dependent Malate Dehydrogenase) negatively correlated with content. Overexpression in resulted lower citrate leaves. Among 15 transcription highly AcPNAD-MDH1, AcSQBP9 (SQUAMOSA PROMOTER-BINDING PROTEIN) shown directly bind promoter repress transcriptional activity. Moreover, targeted CRISPR-Cas9-induced mutagenesis produced significant decrease accompanied by an increase expression. Both PNAD-MDH SQBP have not been widely fruit metabolism, so present omics-oriented study provides insights for both general plant organic acid metabolism.

Language: Английский

Citations

0
1-MCP regulates taste development in cold-stored peach fruit through modulation of sugar, organic acid, and polyphenolic metabolism DOI
Huijuan Zhou,

Zhengwen Ye,

Lufan Wang

et al.

Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 225, P. 113518 - 113518

Published: March 12, 2025

Language: Английский

Citations

0
Multidimensional Transcriptomics Reveals the Key Genes and Pathways Regulating the Acidity of Apples DOI Creative Commons
Wenyuan Yang, Hang Yu, Lian Tao

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(5), P. 341 - 341

Published: May 8, 2025

Low-acid apples are popular among consumers, but the mechanisms behind complex differences in acidity varieties that caused by high altitude not clear. In this study, we used ‘Golden Delicious’ apple and its superior variant Western Sichuan Plateau of China to analyze organic acid composition, content, expression levels related regulated genes during fruit development. We found content was significantly lower than apple. both apples, quinic malic acids were predominant acids, while citric tartaric present amounts. multidimensional regulatory transcriptome sequencing, cluster analysis, weighted gene co-expression network analysis (WGCNA) reveal differentially expressed enriched multiple pathways affecting development; malate dehydrogenase (MDH) affects fruits different varieties; H+-ATPase (VHA) mainly regulates vacuolar which acidity. Additionally, performed qRT-PCR experiments validate our results. This study provides molecular insights into low-acidity traits form offers a theoretical basis for regulating flavor fleshy fruits.

Language: Английский

Citations

0
The accumulation and regulation of malate in fruit cell DOI Creative Commons
Lihua Zhang,

Aoning Zhang,

Xu Yu

et al.

Fruit Research, Journal Year: 2024, Volume and Issue: 4(1), P. 0 - 0

Published: Jan. 1, 2024

Fruit acidity is an important component of flavor quality in fleshy fruit. The accumulation malate, the dominant organic acid formation most mature fruit, highly regulated by metabolism and transportation during fruit development. While, knowledge still limited about mechanism acidification, as well major genes substances. In present paper, research advance on relevance between malate associated with transportation, transcriptional regulation was reviewed. Furthermore, positive future could provide theoretical reference for optimization genetic improvement.

Language: Английский

Citations

3
Expression of the polyphenol oxidase gene MdPPO7 is modulated by MdWRKY3 to regulate browning in sliced apple fruit DOI
Hui Zou,

C. Li,

Xiaoyu Wei

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 197(1)

Published: Nov. 13, 2024

Abstract Browning is a pervasive problem in horticultural products, substantially diminishing the appearance, flavor, and nutritional value of fruit, including important fruits like apple (Malus × domestica Borkh.). In this study, we compared physiological characteristics browning-resistant line ‘Rb-18’ with susceptible variety ‘Fuji’ found that polyphenol oxidase (PPO) enzyme activity phenolic content were significantly lower than those ‘Fuji’. addition, PPO showed stronger affinity for its substrate, catechol, to ‘Rb-18’. Through transcriptome RT-qPCR analyses, MdPPO7 expression was identified as contributing flesh browning after cutting. Subsequent fruit injection stable genetic transformation gene into calli determined syringic acid, procyanidin, phloridzin, chlorogenic gallic catechin, caffeic act catalytic substrates process involved browning. Furthermore, luciferase reporter, yeast 1-hybrid, β-glucuronidase reporter assays ChIP-qPCR analysis demonstrated WRKY transcription factor (MdWRKY3) binds promoter region (MdPPO7) positively regulates promote This study provides insights molecular regulatory mechanisms fresh-cut apples theoretical basis generation high-quality germplasm resources.

Language: Английский

Citations

2
The art of tartness: the genetics of organic acid content in fresh fruits DOI Creative Commons

Shixue Miao,

Xiaoyu Wei, Lingcheng Zhu

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(10)

Published: Aug. 6, 2024

Organic acids are major determinants of fruit flavor and a primary focus crop breeding. The accumulation organic is determined by their synthesis, degradation, transport, all which manipulated sophisticated genetic mechanisms. Constant exploration the basis acid accumulation, especially through linkage analysis, association evolutionary have identified numerous loci in recent decades. In this review, genes responsible for malate citrate contents fruits discussed from perspective. Technologies such as gene transformation genome editing well efficient breeding using marker-assisted selection (MAS) genomic (GS) expected to break bottleneck traditional promote quality improvement.

Language: Английский

Citations

1
Celastrol binds to pyruvate kinase and imitates the metabolic reprogramming of ATP DOI Creative Commons
Xuan Zhang, Xudong Jiang, Xiaobin Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 10, 2024

Summary ATP is the final product of glycolysis and TCA cycle. However, counter-regulation glycosis by poorly understood. Here we show that analog celastrol (CLT) binds to binding pocket on pyruvate kinase PKM (PKM) while inducing allosteric regulation PKM. Quantum calculation predicts hydrogen bond formation between CLT asparagine. Liquid chromatography-mass spectrometry further identifies as CLT’s target. The inhibition stronger longer for weak short ATP. Notably, CLT-PKM interaction perfectly underlies hypoglycemic effects forming glucose carbon flux U turn before Besides, allostery induces a tricarboxylic acid (TCA) vortex which could promote amino lipid degradation energy compensation, leading significant weight loss. Additionally, exerts efficient antioxidant altering strengthen pentose phosphate pathway. Consequently, vividly reproduces ATP-PKM named “ATP resistance” in diabetes pathogenesis.

Language: Английский

Citations

0