Peroxide-induced oxidative stress in erythrocytes under the action of low-frequency vibration DOI Creative Commons
О. И. Доценко, G. V. Taradina

Studia Biologica, Journal Year: 2024, Volume and Issue: 18(4), P. 3 - 20

Published: Dec. 1, 2024

Background. In recent years, many publications have highlighted the role of erythrocytes in pathogenesis various acute and chronic diseases. Their negative impact is explained by ability these cells to generate superoxide anion-radical other reactive oxygen species (ROS) due autooxidation hemoglobin, which increases hypoxia. The purpose this work was study hemoglobin activation redox processes regulation specific physiological under influence vibration – a factor that combines mechanical influence, oxidative stress Materials Methods. An erythrocyte suspension at T = 25 °C subjected for 3 hours frequency range from 8 32 Hz, with amplitudes 0.5±0.04 0.9±0.08 mm. At specified intervals time, content hydrogen peroxide, propensity autoxidation ligand forms cytoplasmic fraction hemolysates were measured. Spearman’s non-parametric correlation analysis used analyze relationship between studied indicators. Results. low-frequen­cy conditions investigated. Changes kinetics reaction oxidation potassium hexacyanoferrate observed. increase intracellular peroxide shown, increased more than twice 16–24 A formation hemichromes, an methemoglobin shown. 20–32 ferrylhemoglobin recorded. Conclusion. Under 8–24 unstable are formed erythrocytes, oxidized hemichromes. process autooxidation, initiates stress, slows down over time oxyhemoglobin. hemichromes high frequencies indicates involvement processes, can consequences cells.

Language: Английский

Mitochondrial regulation of erythropoiesis in homeostasis and disease DOI
Vijay Menon,

Mary Slavinsky,

Olivier Hermine

et al.

British Journal of Haematology, Journal Year: 2024, Volume and Issue: 205(2), P. 429 - 439

Published: June 30, 2024

Summary Erythroid cells undergo a highly complex maturation process, resulting in dynamic changes that generate red blood (RBCs) rich haemoglobin. The end stages of the erythroid cell process primarily include chromatin condensation and nuclear polarization, followed by expulsion called enucleation clearance mitochondria other organelles to finally mature RBCs. While healthy RBCs are devoid mitochondria, recent evidence suggests actively implicated processes maturation, erythroblast RBC production. However, extent mitochondrial participation occurs during these ultimate steps is not completely understood. This specifically important since abnormal retention or DNA contributes pathophysiology sickle disorders. Here we review some key findings so far elucidate importance this various aspects production under homeostasis disease conditions.

Language: Английский

Citations

4
Significant Associations Between Blood Cell Counts and Plasma Cytokines, Chemokines, and Growth Factors DOI Open Access
Lars Eriksson, Mats Eriksson, Torsten Gordh

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4065 - 4065

Published: April 25, 2025

The cytokine network plays a crucial role in regulating immune responses and facilitating intercellular communication. Cytokines are essential numerous physiological pathological processes. This study aimed to investigate associations between blood cell counts broad range of cytokines, chemokines, growth factors. We included one hundred sixty-five essentially healthy individuals this study, which was approved by the Swedish Ethical Review Authority (Dnr 2015/378) registered EudraCT (2014-004235-39). Blood samples were collected for analysis factors using Proseek Multiplex Inflammation kit, Olink Bioscience, Uppsala, Sweden. Correlations different markers calculated Spearman rank correlations, adjusted multiplicity multiple testing, with significance threshold p < 0.05. There significant platelet count 23 white 8 erythrocyte volume fractions 19 cytokines. IL-6 had central within associated platelets also cells neutrophil cells. These findings emphasize integrated nature hematological responses, where parameters systemic activity. observed intercytokine associations, including cross-family interactions, may help highlight regulatory pathways, providing potential targets biomarker development therapeutic intervention immune-mediated conditions.

Language: Английский

Citations

0
Cellular determinants influence the red blood cell adsorption efficiency of poly(amine- co -ester) nanoparticles DOI Creative Commons
Thomas C. Binns, David A. Eaton, Dana V. Akiki

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(18)

Published: May 2, 2025

Many poly(amine- co -ester) (PACE) nanoparticles, drug delivery vehicles for nucleic acid and small molecule cargoes, accumulate in the liver spleen following intravenous administration, limiting to nonhepatosplenic tissues. Red blood cell (RBC) hitchhiking, a strategy which nanoparticles are nonspecifically adsorbed RBCs prior has been used modulate nanoparticle biodistribution, enabling enrichment organs immediately downstream from site of vascular infusion. We find that scarcely investigated cellular determinants—namely, storage duration, membrane stiffness, membrane-bound sialic quantity—substantially affect PACE adsorption efficiency. Following development an optimized protocol, RBC hitchhiking was shown enhance cargo pulmonary tissue while also increasing exposure other assayed organs. These findings inform future study design, implicate variables as potential obstacles or boons clinical translation, demonstrate acids using this with platform.

Language: Английский

Citations

0
DNA remnants in red blood cells enable early detection of cancer DOI
Haobo Sun,

Xingyun Yao,

Yurong Jiao

et al.

Cell Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

Language: Английский

Citations

0
The involvement of mitochondria in erythrocyte pathology and diseases: from mechanisms to therapeutic strategies DOI Creative Commons
Danna Lin, Hongjun Yang,

Xiaoxue Liang

et al.

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 9, 2025

Language: Английский

Citations

0
Correlation of Albumin, Red Cell Distribution Width and Other Biochemical and Hematological Parameters with Glycated Hemoglobin in Diabetic, Prediabetic and Non-Diabetic Patients DOI Open Access
Argyrios Ginoudis, Stavroula Ioannidou,

Georgia Tsakiroglou

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8037 - 8037

Published: July 23, 2024

Diabetes mellitus is a chronic metabolic disease that affects more than 10.5% of the world’s adult population. Biochemical and hematological parameters, such as albumin (ALB) red cell distribution width (RDW), have been shown to be altered in diabetic patients. This study aimed correlate biochemical parameters with glycated hemoglobin (HbA1c). A total 777 adults (372 women 405 men, aged 19–85 years) were divided into three groups: 218 participants HbA1c < 5.7% (group A: non-diabetic), 226 ≥ <6.5% B: prediabetic) 333 6.5% C: diabetic). compared among groups. An analysis variance was performed determine correlations The ALB sodium (Na) levels significantly lower group C groups (ALB: 3.8 g/dL vs. 4.1 g/dL, p 0.0001, Na: 138.4 mmol/L 139.3 mmol/L, 0.001) B 4.0 139.6 0.0001), whereas RDW-standard deviation (RDW-SD) urea increased (RDW: 45.8 43.9 fL, urea: 55.6 mg/dL 38.5 mg/dL, 0.0001). mean platelet volume (MPV) (9.3 fL 9.1 0.05, respectively). Τhe increase RDW-SD demonstrates impact hyperglycemia on blood cells. Albumin RDW might improve risk assessment for development diabetes. These results highlight potential role these an indication prediabetes would alert measurement HbA1c.

Language: Английский

Citations

3
Erythrocytes enhance oxygen-carrying capacity through self-regulation DOI Creative Commons
Ying Xu, Zixiang Yu, Hanxuan Liu

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: May 16, 2025

Once considered passive carriers of oxygen, erythrocytes are now understood to play active roles in regulating oxygen homeostasis and redox balance. This review examines the molecular mechanisms through which red blood cells adapt hypoxic conditions, including nitric oxide (NO)-driven changes membrane properties, βCys93-dependent S-nitrosylation, adenosine-induced activation glycolysis, development memory via eENT1 degradation. Enzymes such as RBC eNOS, CYB5R3, G6PD essential for maintaining NO availability balance by controlling state NADPH synthesis. In addition their role gas transport, contribute intercellular communication, retain organelle remnants under pathological being explored platforms drug delivery. Progress nanotechnology gene editing has expanded clinical applications. These findings present adaptable, multifunctional that connect cellular metabolism, vascular biology, translational research.

Language: Английский

Citations

0
Erythrocyte-related indicators as biomarkers in diabetes: Improvement of erythrocyte function by fruit extracts from different cultivars of the cornelian cherry (Cornus mas L. and Cornus mas × Сornus officinalis hybrids) DOI
Iryna Brodyak, Anna Moroz, Karolina Bernacka

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 188, P. 118194 - 118194

Published: May 23, 2025

Language: Английский

Citations

0
The Role of Ergothioneine in Red Blood Cell Biology: A Review and Perspective DOI Creative Commons

Tiffany Thomas,

Richard O. Francis,

James C. Zimring

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 717 - 717

Published: June 13, 2024

Oxidative stress can damage tissues and cells, their resilience or susceptibility depends on the robustness of antioxidant mechanisms. The latter include small molecules, proteins, enzymes, which are linked together in metabolic pathways. Red blood cells particularly susceptible to oxidative due large number hemoglobin undergo auto-oxidation. This yields reactive oxygen species that participate Fenton chemistry, ultimately damaging membranes cytosolic constituents. Fortunately, red contain robust systems enable them circulate perform physiological functions, delivering removing carbon dioxide. Nonetheless, if have insufficient reserves (e.g., genetics, diet, disease, toxin exposure), this induce hemolysis vivo enhance a “storage lesion” vitro, when donations refrigerator-stored for transfusion purposes. Ergothioneine, molecule not synthesized by mammals, is obtained only through diet. It absorbed from gut enters using highly specific transporter (i.e., SLC22A4). Certain tissues, high ergothioneine levels. Although no deficiency-related disease has been identified, evidence suggests may be beneficial “nutraceutical.” Given requirements resist content, review discusses ergothioneine’s potential importance protecting these identifies knowledge gaps regarding its relevance enhancing cell circulatory, storage, quality.

Language: Английский

Citations

2
Anaerobic glycolysis and oxidative stress interrelation in erythrocytes under administration of Cornus mas L. fruit extracts to rats with streptozotocin-induced diabetes mellitus DOI Creative Commons
Anna Moroz, Iryna Brodyak, Alicja Z. Kucharska

et al.

Studia Biologica, Journal Year: 2024, Volume and Issue: 18(2), P. 49 - 66

Published: June 1, 2024

Background. In diabetes mellitus (DM), analysis of changes in the biochemical profile erythrocytes is important stage complex scientific research to clarify mechanism action medicinal products based on plant raw materials. The fruits Cornus mas L. are widely known. biologically active compounds these show multiple biological effects. However, effect fruit extracts cornelian cherry functional state has not been sufficiently studied. high glucose concentration induces various structural and changes, which lead numerous disturbances their metabolism. Glucose transported into by facilitated diffusion via GLUT2 undergoes catabolic breakdown anaerobic glycolysis (90 % all glucose) pentose phosphate pathway (the rest 10 %). ATP reduced coenzymes NADH + H+ NADPH formed due metabolism participate maintaining structure hemoglobin. Enzymes antioxidant defense system, prevent hemoglobin oxidation methemoglobin, especially important. Hyperglycemia development oxidative stress cause a decrease activity enzymes accumulation ligand forms (HbCO2, MetHb, HbA1c). Therefore, work aimed investigate red yellow content end glycolytic markers status blood cells rats with streptozotocin-induced diabetes. Materials Methods. DM 1 type animals was induced intraperitoneal injection streptozotocin. Experiments were performed male Wistar rats, who, from 10th day after induction, administered per os loganic acid obtained at dose 20 mg/kg body weight for 14 days. On 24th experiment, decapitated under ether anesthesia, taken. pyruvate lactate (as glycolysis) L-lactate dehydrogenase determined plasma erythrocytes, as well (activity superoxide dismutase, catalase glutathione peroxidase, level glutathione, TBA-reactive substances, modifications proteins advanced protein products). Results. catalase, peroxidase significantly increased against proteins, products, pyruvate, L-lactate, administration cherry. Noteworthy, indicators made it possible assess intensity streptozotocin Conclusions. Extracts might be potential natural drugs treatment metabolic disorders diabetes, they have corrective system preventing stress. It should pointed out that extract showed best among studied normalizing indicators.

Language: Английский

Citations

1