Editorial: The expanding Frontiers of stem cells therapy in oral maxillo-facial engineering and regenerative medicine

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 22, 2025

remains a major obstacle to reach successful tissue healing (Li et al., 2024). These challenges underscore the urgent need for innovative, multidisciplinary approaches in engineering and regenerative medicine.Stem cell-based therapies, particularly those utilizing dental-derived mesenchymal stem cells (dMSCs) their bioproducts, have emerged as promising tools advancing oral maxillofacial regeneration 2021). dMSCs, including derived from dental follicle sac, offer distinct advantages due easy access, high proliferative capacity, strong differentiation potential into osteogenic, chondrogenic, neurogenic lineages (Salgado 2020). Additionally, immunomodulatory properties help regulate inflammation, creating conducive environment repair (Costa 2023). In work of Souto-Lopes, authors evaluated vitro osteoinduction periodontal-originating (human -DFMSCs) promoted by nano-hydroxyapatite/chitosan (nHAp/CS) bioaerogel. They also vivo bone capacity biocomposite assessed rat calvaria critical defect model. The results demonstrated bioaerogel had an favorable condition DFMSC proliferation osteogenic differentiation, while showed higher ingrowth. This low environmental impact composite produced eco-friendly process could be further explored alternative biomaterial guiding alveolar regeneration.Therefore, improvement innovative bioactive biomaterials can represent another strategy boost directly influence dMSC fate, leading faster release targeted cell derivates such extracellular vesicles (EVs). EVs human MSC were studied Huang, tool therapeutic translation application, characterized bioactivity before after lyophilization preserve them clinical application instability well suitability stimulating BMP-2 cascade vivo. Results highlighted ability DMSO acting powerful cryopreserving agent prior maintain functional stability engineered serve baseline its therapies. Authors intend future long-term evaluation these DMSO-lyophilized applicability different tissues delivery mechanisms.Another smart produce scaffolds involves multifaceted approach personalize patients' treatments during materials synthesis that provide sustained controlled release, increasing efficacy against pathogens, preventing inflammation infection situ. study Moreno-Flores, al. aimed evaluate behavior marrow assess antimicrobial response 3D printed porous with propolis-modified wollastonite. created through printing triply periodic minimal surface (TPMS) gyroid geometry exhibited mechanical suitable repairing trabecular tissue. impregnating ethanolic extracts propolis (EEPs) effective control over bacterial growth, and, same time, increase MSC's reduction oxidative stress, thus reporting anti-inflammatory properties, too. use natural product sustainable opened new opportunities research, since kept activity minimized on cellular microenvironment reducing stress.To immunomodulation response, Zhang, investigated possibility siRNA expression phosphatase tensin homolog (PTEN) is inflammatory regulation modify as, adipose type II diabetic rats (TADSCs), which impaired capacity. TADSCs upregulation PTEN mRNA expression, but when it was silenced specific siRNA, genes related AKT/mTOR/HIF-1α signaling pathway renews promoting macrophages' M2 polarization decreasing M1 vitro, well. model, inhibition TADSC sheets surrounding titanium implants macrophage toward phenotype, attenuating enhancing osseointegration, suggesting modifying cells' genetic signature patients towards downregulation enhance regeneration.Novel studies craniofacial soft injuries focus reconstruction using biofabrication mature constructs supply current surgical standard. Feinberg & Marcelo used small punch biopsies mucosa source promote lip function improve patient´s rehabilitation quality life. most important goal maintaining circumference viable, well-perfused microvascular flap sufficient bulk perform necessary repairs. requires restoring continuity labial vestibule orbicularis oris muscle (restoration lips' striated volume), intact motor innervation. best occurred completely sphincter active sensory sensation. successfully developed animal model possible develop prelaminated, innervated, pre-vascularized, prefabricated free dynamic, complex, defects lip.Finally, precision stratified medicine pattern analysis datasets are key elements investigate medical treatments. Advanced statistical methods machine learning techniques identify patterns, revealing hypotheses about disease mechanisms or treatment efficacy, match revisions order predicative models supporting clinicians' decisions. By examining adverse events failures population, researchers gaps existing optimization protocols development novel prevention socalled bed-to-bench side data.The Barbaro, found correlation between number occlusal caries anthropometric indexes predicting insulin resistance (IR) nutritional supplement myo-inositol (MYO) might antagonize detrimental effects IR tooth decay. provided feasibility MYO factor dentistry surgery, dysmetabolic/aging conditions, destructive/necrotic disorders, implants, empowering methodologies surgery. reverse translational enables personalized plans based genetic, epigenetic, phenotypic data patient populations. A hard find molecule amelioration reparative/regenerative action. particular, they expected would electively target vascular stromal/MSC-like dental/alveolar/oral tissues. combining vivo, silico studies, concluded has effective, cost, deliver, highly tolerable factor, thus, hard/mineralized tissues, contributing surgery.The advancements therapy solutions, MSCs transforming landscape engineering. manuscripts this special issue collectively illustrate transformative therapies addressing leveraging unique (dMSCs), (EVs), scaffold designs, highlight convergence advanced science medicine. fact, remarkable overcoming challenges, regeneration, stability, modulating immune responses insights predictive models. Their versatility ease accessibility position pivotal developing approaches. Likewise, exemplifies how tailoring solutions overcome hurdles immunomodulation, control.Overall, contributions not only advance understanding interactions, scalable real-world cost-effectiveness, sustainability, accessibility. Despite developments, path fraught demand continued innovation interdisciplinary collaboration, rigorous standardization, comprehensive treatment's safety efficacy. underscores importance integrating methodologies, learning-based models, bridge gap bench research bedside application. integration paradigms, development, computational redefine strategies.Moving forward, collective presented pave clear trajectory unmet needs medicine, setting stage more comprehensive, patient-centered, efficient solutions.

Language: Английский

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Advances in Bone Repair: A Bioengineered PMMA-Based Bone Cement with Enhanced Otseoconductivity Utilizing Human-Derived Bone Powder

Journal of Polymers and the Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Language: Английский

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Assessment of Tissue Changes Observed with Single-Unit Dental Implants Placed in Conjunction with Platelet-Rich Fibrin: An Evaluative Study

Journal of Microscopy and Ultrastructure, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Language: Английский

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Effect of nHA/CS/PLGA delivering adipose stem cell-derived exosomes and bone marrow stem cells on bone healing—in vitro and in vivo studies

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 11, 2024

Adipose stem cell-derived exosomes (ADSC-EXO) have been demonstrated to promote osteogenic differentiation of bone marrow cells (BMSCs) and facilitate regeneration. The present study aims investigate the effect ADSC-EXO-loaded nano-hydroxyapatite/chitosan/poly-lactide-co-glycolide (nHA/CS/PLGA) scaffolds on maxillofacial regeneration using tissue engineering. ADSC-EXO was isolated co-cultured with BMSCs, BMSCs assessed through detection mineralized nodule formation, alkaline phosphatase (ALP) activity, mRNA expression COL1A1 runt-related transcription factor 2 (RUNX2). nHA/CS/PLGA were fabricated loaded these engineering complexes applied defect region rabbits elucidate their effect. markedly enhanced when they ADSC-EXO. This evidenced by an increase in formation ALP In vivo experiments that application effectively repaired defects rabbits. has BMSCs. shown defects. may serve as a potential therapeutic strategy for large-scale

Language: Английский

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Advancements in periodontal pathology: A systematic review and meta-analysis of molecular diagnostics, imaging techniques, regenerative therapies, and artificial intelligence

Journal of Oral Medicine Oral Surgery Oral Pathology and Oral Radiology, Journal Year: 2024, Volume and Issue: 10(4), P. 231 - 237

Published: Dec. 15, 2024

: Periodontal diseases, such as gingivitis and periodontitis, are prevalent conditions that adversely affect the supporting structures of teeth. Recent advancements in field periodontal pathology have enhanced understanding disease mechanisms improved diagnostic therapeutic strategies. To provide a comprehensive review current innovations explore emerging technologies future directions could revolutionize care. This synthesizes findings from recent literature on pathology, including molecular diagnostics, imaging techniques, regenerative therapies, application artificial intelligence (AI). A systematic approach was adopted to identify analyze relevant studies, focusing their clinical relevance potential for transforming Key include: 1. Molecular Diagnostics: Improved identification biomarkers early detection progression monitoring. 2. Imaging Techniques: Enhanced visualization using advanced modalities like cone-beam computed tomography (CBCT) optical coherence (OCT). 3. Regenerative Therapies: Breakthroughs tissue engineering, stem cell therapy, biomaterials regeneration. 4. Artificial Intelligence: Integration AI predictive modeling, support, personalized treatment planning. significantly expanded possibilities. Emerging technologies, approaches, hold immense advancements. Continued research integration these critical enhancing care improving patient outcomes.

Language: Английский

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Zn2SnO4@SiO2@5-FU Nanoparticles as an Additive for Maxillary Bone Defects

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 194 - 194

Published: Dec. 29, 2024

This study investigates the synthesis of Zn2SnO4@SiO2@5-FU nanoparticles as an additive for bone fillers in dental maxillofacial reconstruction. Zn2SnO4 were synthesized and coated with a SiO2 shell, followed by incorporation 5-Fluorouracil (5-FU), aimed at enhancing therapeutic properties classical fillers. Structural analysis using X-ray diffraction confirmed that was single crystalline phase present, its crystallinity preserved after both coating 5-FU incorporation. SEM characterization revealed micro-spherical particles assembled agglomeration nanorods, exhibiting dimensions morphological characteristics consistent addition shell 5-FU. Fourier-transformed infrared spectroscopy provided solid proof successful Zn2SnO4, Zn2SnO4@SiO2, Zn2SnO4@SiO2@5-FU, confirming presence expected functional groups. The layer improved nanoparticle stability solution, indicated zeta potential measurements, while adding significantly increased biocompatibility targeting efficiency. existence is also hydrodynamic diameter, indicating increase particle size incorporating compounds. Antibacterial assays demonstrated selective efficacy against Gram-positive bacteria, showing strongest inhibitory effects. Biofilm inhibition studies further nanoparticles’ effectiveness preventing bacterial colonization. Cytotoxicity tests on A-431 human epidermoid carcinoma cell line dose-dependent reduction viability, highlighting targeted cancer treatment. These findings highlight multifunctional fillers, offering enhanced antimicrobial antitumor capabilities.

Language: Английский

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