HES1 revitalizes the functionality of aged adipose-derived stem cells by inhibiting the transcription of STAT1 DOI Creative Commons
Chengcheng Li, Sen Ren, Chengqi Yan

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 5, 2024

The effectiveness of adipose-derived stem cells (ADSCs) in therapy diminishes with age. It has been reported that transcription factors (TFs) play a crucial role the aging and functionality cells. Nevertheless, there is limited understanding regarding involvement TFs mechanism ADSCs. RNA sequencing (RNA-seq) was utilized to discern differentially expressed genes ADSCs obtained from donors varying ages. exhibiting significant variations across age groups were identified subsequently validated. manipulated exhibit either enhanced expression or reduced levels HES1 STAT1 via lentivirus transfection small interfering (siRNA) techniques. impact these genetic alterations on ADSCs' proliferation, migration, cellular senescence assessed using EdU, transwell, senescence-activated β-galactosidase (SA-β-gal) staining assays. DNA sequences bound by investigated through CUT & Tag assay. Lastly, therapeutic efficacy aged overexpression evaluated skin injury model male Sprague-Dawley rats. 678 showed differential between young old (Y-ADSCs O-ADSCs), 47 being TFs. Notably, TF hairy enhancer split 1 (HES1) notably donors. Introducing resulted improved function suppression senescence, while reducing had opposite effect. Mechanistically, found interact promoter region another TF, signal transducer activator (STAT1), inhibit its transcription. Knocking down could fully reverse negative effects caused decreased ADSCs, leading reduction secretion pro-inflammatory cytokines such as TNF-α, IL-6, IL-8. Ultimately, restoring demonstrated potential promoting wound healing. acts an inhibitor progression modulation expression, suggesting promising avenue for rejuvenating senescent improving

Language: Английский

Therapeutic potential of exosomal lncRNAs derived from stem cells in wound healing: focusing on mesenchymal stem cells DOI Creative Commons

Ali Morabbi,

Mohammad Karimian

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 11, 2025

The self-renewal ability and multipotency of stem cells give them great potential for use in wound healing. Stem cell-derived exosomes, owing to their close biological resemblance parent cells, offer a more efficient, safer, economical approach facilitating cellular communication interactions within different environments. This makes particularly valuable the treatment both acute chronic wounds, such as lacerations, burns, diabetic ulcers. Long non-coding RNAs (lncRNAs) enclosed one leading actors these extracellular microvesicles, through interaction with miRNAs regulation various signaling pathways involved inflammation, angiogenesis, cell proliferation, migration, could heal wounds. Exosome-derived lncRNAs from facilitate matrix remodeling between macrophages fibroblasts. Moreover, alongside regulating expression inflammatory cytokines, controlling reactive oxygen species levels, enhancing autophagic activity, they also modulate immune responses support Regulating genes related by increasing blood supply accelerating delivery essential substances environment, is another effect exosomal derived These can enhance skin healing homeostasis, proliferation differentiation wound-healing process, fibroblast viability migration injury site. Ultimately, exosome-derived novel insights into molecular mechanisms underlying improved They pave way therapeutic strategies, fostering further research better future. Meanwhile, exosomes mesenchymal due exceptional regenerative properties, well have emerged innovative tools review article aims narrate roles focus on cells.

Language: Английский

Citations

0
Senescence in Adipose-Derived Stem Cells: Biological Mechanisms and Therapeutic Challenges DOI Open Access
Riccardo Foti, Gabriele Storti,

Marco Palmesano

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8390 - 8390

Published: Aug. 1, 2024

Adipose tissue-derived stem cells (ADSCs) represent a subset of the mesenchymal in every adipose compartment throughout body. ADSCs can differentiate into various cell types, including chondrocytes, osteocytes, myocytes, and adipocytes. Moreover, they exhibit notable potential to vitro from other germinal lineages, endothelial neurons. have wide range clinical applications, breast surgery chronic wounds. Furthermore, are promising population for future tissue-engineering uses. Accumulating evidence indicates decreased proliferation differentiation with an increasing age, body mass index, diabetes mellitus, metabolic syndrome, or exposure radiotherapy. Therefore, recent literature thoroughly investigates this population’s senescence mechanisms how hinder its possible therapeutic applications. This review will discuss biological physio-pathological causes behind ADSC impact cellular functionality. we examine strategies invert these processes, re-establishing full regenerative progenitor population.

Language: Английский

Citations

2
Pseudogene: Relevant or Irrelevant? DOI Creative Commons
Yang-Hsiang Lin,

Chau‐Ting Yeh,

Cheng‐Yi Chen

et al.

Biomedical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 100790 - 100790

Published: Sept. 1, 2024

Language: Английский

Citations

2
HES1 revitalizes the functionality of aged adipose-derived stem cells by inhibiting the transcription of STAT1 DOI Creative Commons
Chengcheng Li, Sen Ren, Chengqi Yan

et al.

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 5, 2024

The effectiveness of adipose-derived stem cells (ADSCs) in therapy diminishes with age. It has been reported that transcription factors (TFs) play a crucial role the aging and functionality cells. Nevertheless, there is limited understanding regarding involvement TFs mechanism ADSCs. RNA sequencing (RNA-seq) was utilized to discern differentially expressed genes ADSCs obtained from donors varying ages. exhibiting significant variations across age groups were identified subsequently validated. manipulated exhibit either enhanced expression or reduced levels HES1 STAT1 via lentivirus transfection small interfering (siRNA) techniques. impact these genetic alterations on ADSCs' proliferation, migration, cellular senescence assessed using EdU, transwell, senescence-activated β-galactosidase (SA-β-gal) staining assays. DNA sequences bound by investigated through CUT & Tag assay. Lastly, therapeutic efficacy aged overexpression evaluated skin injury model male Sprague-Dawley rats. 678 showed differential between young old (Y-ADSCs O-ADSCs), 47 being TFs. Notably, TF hairy enhancer split 1 (HES1) notably donors. Introducing resulted improved function suppression senescence, while reducing had opposite effect. Mechanistically, found interact promoter region another TF, signal transducer activator (STAT1), inhibit its transcription. Knocking down could fully reverse negative effects caused decreased ADSCs, leading reduction secretion pro-inflammatory cytokines such as TNF-α, IL-6, IL-8. Ultimately, restoring demonstrated potential promoting wound healing. acts an inhibitor progression modulation expression, suggesting promising avenue for rejuvenating senescent improving

Language: Английский

Citations

0