Characterization of Fibronectin-Adherent, Non-Fibronectin-Adherent, and Explant-Derived Human Dental Pulp Stem Cell Populations DOI Creative Commons
Heebal Kim, Shelley Williams,

John S. Colombo

et al.

Dentistry Journal, Journal Year: 2025, Volume and Issue: 13(4), P. 159 - 159

Published: April 2, 2025

Background/Objectives: Dental pulp stem cells (DPSCs) are of significant interest due to their mesenchymal lineage and relative availability from extracted teeth. This study aims examine the relationship between fibronectin-adherent, non-fibronectin-adherent, explant-derived DPSC populations in terms population doubling rate culture expression cell surface markers capacity for osteodifferentiation. Methods: Human tissue was removed healthy human teeth, enzymatically digested prior seeding onto fibronectin-coated plates, left adhere 20 min, yielding a fibronectin-adherent population. The remaining non-adherent were transferred designated ‘non-fibronectin-adherent.’ Intact placed on uncoated plastic 5 days, with migrated ‘explant-derived’. DPSCs these examined rates, CD90, CD44, CD105, CD73, RUNX2, SPP1, BGLAP after 7 days osteoinductive media. Results: had greatest over time. All demonstrated comparable percentages positive markers, though individual marker varied slightly. showed increased RUNX2 media, while treated single-cell-suspension-derived SPP1 mRNA. Conclusions: Fibronectin enrichment resulted extended compared other two populations. proportion all four same cultures may have responded more rapidly media than cells.

Language: Английский

Activation of PI3K-AKT pathway prevents steroid-induced osteonecrosis of the femoral head via inhibiting Cuproptosis DOI Creative Commons
Shihua Gao, Haoran Zhu, Haishan Chen

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 15, 2025

Language: Английский

Citations

0
Characterization of Fibronectin-Adherent, Non-Fibronectin-Adherent, and Explant-Derived Human Dental Pulp Stem Cell Populations DOI Creative Commons
Heebal Kim, Shelley Williams,

John S. Colombo

et al.

Dentistry Journal, Journal Year: 2025, Volume and Issue: 13(4), P. 159 - 159

Published: April 2, 2025

Background/Objectives: Dental pulp stem cells (DPSCs) are of significant interest due to their mesenchymal lineage and relative availability from extracted teeth. This study aims examine the relationship between fibronectin-adherent, non-fibronectin-adherent, explant-derived DPSC populations in terms population doubling rate culture expression cell surface markers capacity for osteodifferentiation. Methods: Human tissue was removed healthy human teeth, enzymatically digested prior seeding onto fibronectin-coated plates, left adhere 20 min, yielding a fibronectin-adherent population. The remaining non-adherent were transferred designated ‘non-fibronectin-adherent.’ Intact placed on uncoated plastic 5 days, with migrated ‘explant-derived’. DPSCs these examined rates, CD90, CD44, CD105, CD73, RUNX2, SPP1, BGLAP after 7 days osteoinductive media. Results: had greatest over time. All demonstrated comparable percentages positive markers, though individual marker varied slightly. showed increased RUNX2 media, while treated single-cell-suspension-derived SPP1 mRNA. Conclusions: Fibronectin enrichment resulted extended compared other two populations. proportion all four same cultures may have responded more rapidly media than cells.

Language: Английский

Citations

0